Molecular characterization of bovine Cryptosporidium using Cryptosporidium oocyst wall protein (COWP) gene

Cryptosporidium is a globally distributed protozoan parasite and one of the most common causes of infection and diarrhea in humans and cattle. The aim of the present study was to determine the species of Cryptosporidium among cattle with diarrhea by a nested PCR-RFLP technique at Cryptosporidium oocyst wall protein ( COWP ). Fecal samples from 158 calves aged 1-20 weeks were collected from 10 dairy farms in Qazvin province, Iran. Initial identification of Cryptosporidium was carried out by Zeihl-Neelsen acid-fast staining method of stool samples. DNA was extracted from 26 (16.45 %) positive microscopically samples and Cryptosporidium genotypes were determined. Cryptosporidium parvum were identified in 80.8% of the positive samples and, Cryptosporidium andersoni in 19.2%. In conclusion the use of COWP primers could be sensitive enough to conduct a routine detection study. The nested PCR method using the COWP gene sequence can be an alternative diagnostic method to identify infected with Cryptosporidium and its genetic diversity.

[1]  F. Tahvildar-biderouni,et al.  Detection of Cryptosporidium infection by modified ziehl-neelsen and PCR methods in children with diarrheal samples in pediatric hospitals in Tehran , 2014, Gastroenterology and hepatology from bed to bench.

[2]  Lihua Xiao,et al.  The importance of subtype analysis of Cryptosporidium spp. in epidemiological investigations of human cryptosporidiosis in Iran and other Mideast countries , 2012, Gastroenterology and hepatology from bed to bench.

[3]  M. Zali,et al.  Subtype analysis of Cryptosporidium parvum and Cryptosporidium hominis isolates from humans and cattle in Iran. , 2011, Veterinary parasitology.

[4]  M. Zali,et al.  DNA FINGERPRINTING OF BOVINE CRYPTOSPORIDIUM ISOLATES IN QAZVIN PROVINCE, IRAN , 2011 .

[5]  B. Kazemi,et al.  Genotyping of Cryptosporidium spp. in clinical samples: PCR-RFLP analysis of the TRAP-C2 gene , 2011, Gastroenterology and hepatology from bed to bench.

[6]  B. Kazemi,et al.  Prevalence and molecular characterization of bovine Cryptosporidium in Qazvin province, Iran. , 2009, Veterinary parasitology.

[7]  S. Cacciò,et al.  Distribution of Cryptosporidium parvum subtypes in calves in Germany. , 2008, Veterinary parasitology.

[8]  J. Sadraei,et al.  Molecular characterization of Cryptosporidium isolates from human and bovine using 18s rRNA gene in Shahriar county of Tehran, Iran , 2008, Parasitology Research.

[9]  D. Nguyen,et al.  Prevalence and first genetic identification of Cryptosporidium spp. in cattle in central Viet Nam. , 2007, Veterinary parasitology.

[10]  R. Salehi,et al.  The identification of Cryptosporidium species in Isfahan, Iran by PCR-RFLP analysis of the 18S rRNA gene , 2007, Molecular Biology.

[11]  M. Azami.  Prevalence of Cryptosporidium Infection in Cattle in Isfahan, Iran , 2007, The Journal of eukaryotic microbiology.

[12]  K. Mohammad,et al.  Molecular Characterization of Cryptosporidium Isolates from Humans and Animals in Iran , 2006, Applied and Environmental Microbiology.

[13]  J. Vítovec,et al.  Age-related and housing-dependence of Cryptosporidium infection of calves from dairy and beef herds in South Bohemia, Czech Republic. , 2006, Veterinary parasitology.

[14]  S. Martin,et al.  Genotype and subtype analyses of Cryptosporidium isolates from dairy calves and humans in Ontario , 2006, Parasitology Research.

[15]  R. Fayer,et al.  Prevalence of species and genotypes of Cryptosporidium found in 1-2-year-old dairy cattle in the eastern United States. , 2006, Veterinary parasitology.

[16]  R. Fayer,et al.  CRYPTOSPORIDIUM BOVIS N. SP. (APICOMPLEXA: CRYPTOSPORIDIIDAE) IN CATTLE (BOS TAURUS) , 2005, The Journal of parasitology.

[17]  R. Fayer,et al.  Prevalence and age-related variation of Cryptosporidium species and genotypes in dairy calves. , 2004, Veterinary parasitology.

[18]  Una Ryan,et al.  Cryptosporidium Taxonomy: Recent Advances and Implications for Public Health , 2004, Clinical Microbiology Reviews.

[19]  H. Mohammed,et al.  Utility of the Cryptosporidium oocyst wall protein (COWP) gene in a nested PCR approach for detection infection in cattle. , 2003, Veterinary parasitology.

[20]  F. Sevinc,et al.  The prevalence of Cryptosporidium parvum infection in the diarrhoiec and non- diarrhoeic calves , 2003 .

[21]  E. Ares-Mazás,et al.  A study of cryptosporidiosis in a cohort of neonatal calves. , 2002, Veterinary parasitology.

[22]  J. McLauchlin,et al.  Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene. , 2001, Emerging infectious diseases.

[23]  M. Nouri,et al.  Asymptomatic cryptosporidiosis in cattle and humans in Iran , 1991, Veterinary Record.