An Hybrid Micro-Force Sensing Device for Mechanical Cell Characterization

This paper presents a fully automated microrobotic system based on force/vision referenced control designed for cell mechanical characterization. The design of the prototype combines scanning probe microscopy (SPM) techniques with advanced robotics approaches. As a result, accurate and non-destructive mechanical characterization based on soft contact interactions mechanics are achieved. The in vitro working conditions are supported by the experimental setup so that mechanical characterizations can be performed in biological environmental requirements as well as in cyclical operating mode during several hours. The design and calibration of the different modules which compose the experimental setup are detailed. Experimentation on the mechanical cell characterization under in vitro conditions on human adherent cervix Epithelial Hela cells are presented to demonstrate the viability and effectiveness of the proposed setup

[1]  Jochen Guck,et al.  Stretching biological cells with light , 2002 .

[2]  Manfred H. Jericho,et al.  Atomic force microscopy and theoretical considerations of surface properties and turgor pressures of bacteria , 2002 .

[3]  Seonghun Park,et al.  Microscale frictional response of bovine articular cartilage from atomic force microscopy. , 2004, Journal of biomechanics.

[4]  Olav Solgaard,et al.  High-resolution imaging of elastic properties using harmonic cantilevers , 2004 .

[5]  김병규,et al.  Mechanical Force Response of Single Living Cells using a Microrobotic System , 2004 .

[6]  Bradley J. Nelson,et al.  Microrobotic cell injection , 2001, Proceedings 2001 ICRA. IEEE International Conference on Robotics and Automation (Cat. No.01CH37164).

[7]  Ferenc Horkay,et al.  Determination of elastic moduli of thin layers of soft material using the atomic force microscope. , 2002, Biophysical journal.

[8]  Bradley J. Nelson,et al.  Mems for Cellular Force Measurements and Molecular Detection , 2004, Int. J. Inf. Acquis..

[9]  Subra Suresh,et al.  The biomechanics toolbox: experimental approaches for living cells and biomolecules , 2003 .

[10]  M Radmacher,et al.  Measuring the elastic properties of biological samples with the AFM. , 1997, IEEE engineering in medicine and biology magazine : the quarterly magazine of the Engineering in Medicine & Biology Society.

[11]  Pasi Kallio,et al.  Multi-purpose impedance-based measurement system to automate microinjection of adherent cells , 2005, 2005 International Symposium on Computational Intelligence in Robotics and Automation.