Patterns of cellular interactions during fruiting-body formation in Myxococcus xanthus

Aggregation and mound formation during development of the myxobacterium Myxococcus xanthus were examined by scanning electron microscopy and light microscopy. Several complex patterns of multicellular associations were observed. These observations imply that complex, organized cell-cell interactions occur during the process of development. Examination of sliced aggregates revealed that, contrary to common perception, the process of sporulation commenced during mound formation rather than after the completion of mound morphogenesis. The morphogenesis of M. xanthus fruiting bodies is compared with the morphogenesis of fruiting bodies of other members of the Myxobacteriales previously described in the literature.

[1]  L. Shimkets.  Control of morphogenesis in myxobacteria. , 1987, Critical reviews in microbiology.

[2]  D. Kaiser,et al.  Induction of coordinated movement of Myxococcus xanthus cells , 1982, Journal of bacteriology.

[3]  D. Zusman,et al.  Myxococcus xanthus Mutants with Temperature-Sensitive, Stage-Specific Defects: Evidence for Independent Pathways in Development , 1979, Journal of bacteriology.

[4]  D. Zusman,et al.  Reexamination of the role of autolysis in the development of Myxococcus xanthus , 1988, Journal of bacteriology.

[5]  D. Zusman,et al.  "Frizzy" aggregation genes of the gliding bacterium Myxococcus xanthus show sequence similarities to the chemotaxis genes of enteric bacteria. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[6]  S. Inouye,et al.  Tandem repeat of the genes for protein S, a development-specific protein of Myxococcus xanthus. , 1983, The Journal of biological chemistry.

[7]  R. Gill,et al.  Control of developmental gene expression by cell-to-cell interactions in Myxococcus xanthus , 1986, Journal of bacteriology.

[8]  D. Zusman,et al.  Isolation of bacteriophage MX4, a generalized transducing phage for Myxococcus xanthus. , 1978, Journal of molecular biology.

[9]  J. Pangborn,et al.  Scanning electron microscopy of fruiting body formation by myxobacteria , 1975, Journal of bacteriology.

[10]  M. Dworkin.  NUTRITIONAL REQUIREMENTS FOR VEGETATIVE GROWTH OF MYXOCOCCUS XANTHUS , 1962, Journal of bacteriology.

[11]  D. Zusman,et al.  Nucleotide sequence of the myxobacterial hemagglutinin gene contains four homologous domains. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[12]  M. Dworkin,et al.  Developmentally induced autolysis during fruiting body formation by Myxococcus xanthus , 1977, Journal of bacteriology.

[13]  D. Kaiser,et al.  Synergism between morphogenetic mutants of Myxococcus xanthus. , 1978, Developmental biology.

[14]  D. White,et al.  Morphogenesis of Stigmatella aurantiaca fruiting bodies , 1985, Journal of bacteriology.

[15]  J. Bonner,et al.  Acrasin, Acrasinase, and the sensitivity to acrasin in Dictyostelium discoideum. , 1969, Developmental biology.

[16]  A. Kuspa,et al.  A global analysis of developmentally regulated genes in Myxococcus xanthus. , 1986, Developmental biology.

[17]  M. Dworkin,et al.  Morphogenesis and developmental interactions in myxobacteria. , 1975, Science.

[18]  D. Zusman,et al.  Myxobacterial hemagglutinin: a development-specific lectin of Myxococcus xanthus. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[19]  S. Inouye,et al.  Biosynthesis and self-assembly of protein S, a development-specific protein of Myxococcus xanthus. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[20]  D. Zusman,et al.  "Frizzy" mutants: a new class of aggregation-defective developmental mutants of Myxococcus xanthus , 1982, Journal of bacteriology.

[21]  J. R. Lampky.  Ultrastructure of Polyangium cellulosum , 1976, Journal of bacteriology.

[22]  D. Kaiser,et al.  Fruiting body morphogenesis in submerged cultures of Myxococcus xanthus , 1982, Journal of bacteriology.