Structural basis of X chromosome DNA recognition by the MSL2 CXC domain during Drosophila dosage compensation

The male-specific lethal dosage compensation complex (MSL-DCC) selectively assembles on the X chromosome in Drosophila and activates gene transcription by twofold through histone acetylation. An MSL recognition element (MRE) sequence motif nucleates the initial MSL association. Here, Zheng et al. identified the CXC domain of MSL2 specifically recognizing the MRE motif and determined its crystal structure bound to specific and nonspecific DNAs. Specific DNA-binding mutants of MSL2 are impaired in MRE binding and X chromosome localization in vivo.

[1]  T. Cech,et al.  A dimeric state for PRC2 , 2014, Nucleic acids research.

[2]  Zhaohui S. Qin,et al.  H2B ubiquitylation promotes RNA Pol II processivity via PAF1 and pTEFb. , 2014, Molecular cell.

[3]  M. Kuroda,et al.  Are we there yet? Initial targeting of the Male-Specific Lethal and Polycomb group chromatin complexes in Drosophila , 2014, Open Biology.

[4]  C. Ellison,et al.  Dosage Compensation via Transposable Element Mediated Rewiring of a Regulatory Network , 2013, Science.

[5]  P. Becker,et al.  ATP-dependent roX RNA remodeling by the helicase maleless enables specific association of MSL proteins. , 2013, Molecular cell.

[6]  Michael Y Tolstorukov,et al.  The CLAMP protein links the MSL complex to the X chromosome during Drosophila dosage compensation. , 2013, Genes & development.

[7]  P. Park,et al.  Conservation and de novo acquisition of dosage compensation on newly evolved sex chromosomes in Drosophila. , 2013, Genes & development.

[8]  William Stafford Noble,et al.  Mammalian X upregulation is associated with enhanced transcription initiation, RNA half-life, and MOF-mediated H4K16 acetylation. , 2013, Developmental cell.

[9]  G. Gilfillan,et al.  Different chromatin interfaces of the Drosophila dosage compensation complex revealed by high-shear ChIP-seq , 2013, Genome research.

[10]  S. Cusack,et al.  Msl1-mediated dimerization of the dosage compensation complex is essential for male X-chromosome regulation in Drosophila. , 2012, Molecular cell.

[11]  R. Villa,et al.  MSL2 combines sensor and effector functions in homeostatic control of the Drosophila dosage compensation machinery. , 2012, Molecular cell.

[12]  C. Disteche.  Dosage compensation of the sex chromosomes. , 2012, Annual review of genetics.

[13]  K. Ye,et al.  Solution Structure of MSL2 CXC Domain Reveals an Unusual Zn3Cys9 Cluster and Similarity to Pre-SET Domains of Histone Lysine Methyltransferases , 2012, PloS one.

[14]  P. Park,et al.  Identification of Chromatin-Associated Regulators of MSL Complex Targeting in Drosophila Dosage Compensation , 2012, PLoS genetics.

[15]  P. Park,et al.  Sequence-Specific Targeting of Dosage Compensation in Drosophila Favors an Active Chromatin Context , 2012, PLoS genetics.

[16]  B. Garcia,et al.  The RING finger protein MSL2 in the MOF complex is an E3 ubiquitin ligase for H2B K34 and is involved in crosstalk with H3 K4 and K79 methylation. , 2011, Molecular cell.

[17]  T. Straub,et al.  Transcription modulation chromosome-wide: universal features and principles of dosage compensation in worms and flies. , 2011, Current opinion in genetics & development.

[18]  S. Cusack,et al.  Structural basis for MOF and MSL3 recruitment into the dosage compensation complex by MSL1 , 2011, Nature Structural &Molecular Biology.

[19]  Seung‐Won Park,et al.  MSL cis-spreading from roX gene up-regulates the neighboring genes. , 2010, Biochemical and biophysical research communications.

[20]  T. Straub,et al.  The DNA binding CXC domain of MSL2 is required for faithful targeting the Dosage Compensation Complex to the X chromosome , 2010, Nucleic acids research.

[21]  P. Park,et al.  Long-range spreading of dosage compensation in Drosophila captures transcribed autosomal genes inserted on X. , 2009, Genes & development.

[22]  S. Gaubatz,et al.  LIN54 is an essential core subunit of the DREAM/LINC complex that binds to the cdc2 promoter in a sequence‐specific manner , 2009, The FEBS journal.

[23]  R. Mann,et al.  The role of DNA shape in protein-DNA recognition , 2009, Nature.

[24]  M. Kuroda,et al.  Drosophila dosage compensation: a complex voyage to the X chromosome , 2009, Development.

[25]  G. Gilfillan,et al.  The Chromosomal High-Affinity Binding Sites for the Drosophila Dosage Compensation Complex , 2008, PLoS genetics.

[26]  Peter J. Park,et al.  A Sequence Motif within Chromatin Entry Sites Directs MSL Establishment on the Drosophila X Chromosome , 2008, Cell.

[27]  M. Cristina Cardoso,et al.  A Versatile Nanotrap for Biochemical and Functional Studies with Fluorescent Fusion Proteins*S , 2008, Molecular & Cellular Proteomics.

[28]  M. J. Scott,et al.  Incorporation of the Noncoding roX RNAs Alters the Chromatin-Binding Specificity of the Drosophila MSL1/MSL2 Complex , 2007, Molecular and Cellular Biology.

[29]  B. van Steensel,et al.  Chromosome-wide gene-specific targeting of the Drosophila dosage compensation complex. , 2006, Genes & development.

[30]  Malgorzata Schelder,et al.  Nuclear pore components are involved in the transcriptional regulation of dosage compensation in Drosophila. , 2006, Molecular cell.

[31]  William Arbuthnot Sir Lane,et al.  A Human Protein Complex Homologous to the Drosophila MSL Complex Is Responsible for the Majority of Histone H4 Acetylation at Lysine 16 , 2006, Molecular and Cellular Biology.

[32]  William Arbuthnot Sir Lane,et al.  A Human Protein Complex Homologous to the Drosophila MSL Complex Is Responsible for the Majority of Histone H4 Acetylation at Lysine 16 , 2005, Molecular and Cellular Biology.

[33]  E. Kremmer,et al.  Stable chromosomal association of MSL2 defines a dosage-compensated nuclear compartment , 2005, Chromosoma.

[34]  P. Lichter,et al.  hMOF Histone Acetyltransferase Is Required for Histone H4 Lysine 16 Acetylation in Mammalian Cells , 2005, Molecular and Cellular Biology.

[35]  R. Kaptein,et al.  Structure and Flexibility Adaptation in Nonspecific and Specific Protein-DNA Complexes , 2004, Science.

[36]  T. Straub,et al.  Functional integration of the histone acetyltransferase MOF into the dosage compensation complex , 2004, The EMBO journal.

[37]  M. Kuroda,et al.  Local spreading of MSL complexes from roX genes on the Drosophila X chromosome. , 2003, Genes & development.

[38]  I. Marín.  Evolution of Chromatin-Remodeling Complexes: Comparative Genomics Reveals the Ancient Origin of “Novel” Compensasome Genes , 2003, Journal of Molecular Evolution.

[39]  R. Kelley,et al.  Extent of Chromatin Spreading Determined by roX RNA Recruitment of MSL Proteins , 2002, Science.

[40]  R. Kelley,et al.  Association and spreading of the Drosophila dosage compensation complex from a discrete roX1 chromatin entry site , 2001, The EMBO journal.

[41]  K. Larsen,et al.  CPP1, a DNA-binding protein involved in the expression of a soybean leghemoglobin c3 gene. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[42]  R. Kelley,et al.  Ordered assembly of roX RNAs into MSL complexes on the dosage-compensated X chromosome in Drosophila , 2000, Current Biology.

[43]  M. Kuroda,et al.  Complex formation by the Drosophila MSL proteins: role of the MSL2 RING finger in protein complex assembly , 1998, The EMBO journal.

[44]  R. Kelley,et al.  Drosophila male-specific lethal-2 protein: structure/function analysis and dependence on MSL-1 for chromosome association. , 1997, Genetics.

[45]  A. Lane,et al.  Structural aspects of protein-DNA recognition. , 1991, The Biochemical journal.

[46]  N. Pavletich,et al.  Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A , 1991, Science.

[47]  Eric Blanc,et al.  Automated structure solution with autoSHARP. , 2007, Methods in molecular biology.

[48]  Bruce A Johnson,et al.  Using NMRView to visualize and analyze the NMR spectra of macromolecules. , 2004, Methods in molecular biology.

[49]  Lee Fielding,et al.  NMR methods for the determination of protein-ligand dissociation constants. , 2003, Current topics in medicinal chemistry.

[50]  C. Pabo,et al.  DNA recognition by Cys2His2 zinc finger proteins. , 2000, Annual review of biophysics and biomolecular structure.

[51]  K. Wilson,et al.  Efficient anisotropic refinement of macromolecular structures using FFT. , 1999, Acta crystallographica. Section D, Biological crystallography.

[52]  J. Lucchesi,et al.  Targeting of MOF, a putative histone acetyl transferase, to the X chromosome of Drosophila melanogaster. , 1998, Developmental genetics.

[53]  Z. Otwinowski,et al.  Processing of X-ray diffraction data collected in oscillation mode. , 1997, Methods in enzymology.