Characterization of powdered turmeric by liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry

Five commercial powdered turmeric samples were analyzed to identify major and minor components. The developed HPLC method allows the separation of curcumin, demethoxycurcumin and bisdemethoxycurcumin, as well as three other major components and numerous minor components. The separation was accomplished on an octadecyl stationary phase using a mobile phase consisting of 50 mM ammonium acetate with 5% acetic acid and acetonitrile as the organic modifier. Thermospray mass spectra were obtained for all of the components. Particle beam EI-mass spectra were obtained for the curcuminoids, but could not be obtained for the other components due to the limitations of the particle beam interface when analyzing volatile and semi-volatile compounds. EI mass spectra for the volatile components were obtained by direct thermal desorption-gas chromatography-mass spectrometry (DTD-GC-MS).

[1]  T. Miyase,et al.  Natural antioxidants. III. Antioxidative components isolated from rhizome of Curcuma longa L. , 1985, Chemical & pharmaceutical bulletin.

[2]  Chi-Tang Ho,et al.  High Performance Liquid Chromatographic Analysis of Curcuminoids and Their Photo-oxidative Decomposition Compounds in Curcuma Longa L , 1988 .

[3]  R. Seshadri,et al.  Volatile aroma components of Curcuma amada Roxb. , 1989 .

[4]  S. Bhide,et al.  Chemopreventive effect of turmeric against stomach and skin tumors induced by chemical carcinogens in Swiss mice. , 1992, Nutrition and cancer.

[5]  Roger M. Smith,et al.  Application of supercritical fluid extraction and chromatography to the analysis of turmeric , 1993 .

[6]  G. Jilani,et al.  Isolation, purification, and characterization of insect repellents from Curcuma longa L , 1982 .

[7]  D. Kingston,et al.  Intramolecular hydrogen transfer in mass spectra. II. McLafferty rearrangement and related reactions , 1974 .

[8]  V S Govindarajan,et al.  Turmeric--chemistry, technology, and quality. , 1980, Critical reviews in food science and nutrition.

[9]  S. J. Taylor,et al.  Determination of the curcuminoid pigments in turmeric (Curcuma domestica Val) by reversed-phase high-performance liquid chromatography , 1992 .

[10]  Y. Oshima,et al.  Stereostructure of curlone, a sesquiterpenoid of Curcuma longa rhizomes , 1983 .

[11]  R. Rouseff.  High Performance Liquid Chromatographic Separation and Spectral Characterization of the Pigments in Turmeric and Annatto , 1988 .

[12]  H. Tønnesen,et al.  High-performance liquid chromatography of curcumin and related compounds , 1983 .

[13]  R. T. Rosen,et al.  Application of particle beam LC/MS for the analysis of water from publicly owned treatment works , 1991 .

[14]  Irwin H. Suffet,et al.  Determination of Alkylphenol Ethoxylates and Their Acetic Acid Derivatives in Drinking Water by Particle Beam Liquid Chromatography/Mass Spectrometry , 1992 .

[15]  H. Nursten,et al.  Comparative study of the reversed-phase high-performance liquid chromatography of black tea liquors with special reference to the thearubigins , 1991 .

[16]  P. Majlát,et al.  Calculation and application of the retention indices in programmed-temperature gas chromatography , 1974 .

[17]  Roger M. Smith,et al.  Comparison of detectors for the determination of curcumin in turmeric by high-performance liquid chromatography , 1984 .