An imaging fiber-based optical tweezer array for microparticle array assembly

In this letter, we present a method to generate and regenerate arrays of microspheres by optically trapping through optical imaging fiber bundles. In this method, a laser beam is coupled into the proximal end of an optical fiber bundle, and the light energy is distributed across the face of the fiber. Each illuminated individual fiber in the array propagates light to the distal face of the bundle, where light focusing elements at the end of each fiber focus the laser light and form optical traps. These optical traps are capable of capturing and arraying microspheres in parallel. The number of optical traps is determined by the number of fibers in the optical fiber bundle and is capable of creating a dense array (∼5×104 traps/mm2) of optical tweezers.

[1]  A. Ashkin,et al.  Optical trapping and manipulation of single cells using infrared laser beams , 1987, Nature.

[2]  J. Golovchenko,et al.  Optical Matter: Crystallization and Binding in Intense Optical Fields , 1990, Science.

[3]  S. P. Fodor DNA SEQUENCING: Massively Parallel Genomics , 1997, Science.

[4]  Hiroshi Masuhara,et al.  Laser-Scanning Micromanipulation and Spatial Patterning of Fine Particles , 1991 .

[5]  Andrew J. Holloway,et al.  Options available—from start to finish—for obtaining data from DNA microarrays II , 2002, Nature Genetics.

[6]  Arthur Ashkin,et al.  Optical Levitation by Radiation Pressure , 1971 .

[7]  G. Cagney,et al.  Large-scale functional analysis using peptide or protein arrays , 2000, Nature Biotechnology.

[8]  E. Lyons,et al.  Confinement and bistability in a tapered hemispherically lensed optical fiber trap , 1995 .

[9]  A. Ashkin,et al.  Optical trapping and manipulation of viruses and bacteria. , 1987, Science.

[10]  David R. Walt,et al.  Ordered nanowell arrays , 1996 .

[11]  J Tanida,et al.  Optical manipulation of microscopic objects by means of vertical-cavity surface-emitting laser array sources. , 2001, Applied optics.

[12]  A. Ashkin Forces of a single-beam gradient laser trap on a dielectric sphere in the ray optics regime. , 1992, Methods in cell biology.

[13]  K. Svoboda,et al.  Biological applications of optical forces. , 1994, Annual review of biophysics and biomolecular structure.

[14]  D. Grier,et al.  Optical tweezer arrays and optical substrates created with diffractive optics , 1998 .