Response of metabolic enzymes glutamic oxaloacetate transaminase and glutamate pyruvate transaminase in vivo exposed Oreochromis niloticus against organophosphates and synthetic pyrethroid

Organophosphates and synthetic pyrethroid pesticides are used in residential areas and agriculture, discharge into environment, may cause unpredictable toxicity to non-targeted aquatic organisms. The current study aims to assess the effect of these pesticides on the transaminases, glutamate oxaloacetate transaminase and glutamate pyruvate transaminase enzymes activity in brain and gills of Oreochromis niloticus. Fish were exposed for 24 and 48 h (hours) to the LC0 (non-lethal concentrations) of the malathion, chlorpyrifos and λ-cyhalothrin, respectively. For biochemical assay, a spectrophotometer was used. Glutamate oxaloacetate transaminase activity in brain and gill was significantly (p<0.05) elevated and inhibited, respectively, except in 48 h treated samples. The highest (+258%) and lowest (+8.9%) enhancement in this activity was noted in chlorpyrifos and malathion treated gill samples, respectively. The glutamate pyruvate transaminase activity was significantly (p<0.05) enhanced in all treated samples except in 24 h malathion treated brain. The highest (+340.7%) and lowest (+27%) enhancement in this level was recorded in malathion and chlorpyrifos exposed brain and gills samples. Therefore, it is suggested that both these assessments in fish different organs could be used as a useful biomarker to detect environmental pollution. Thus, the alterations in transaminases activity indicated that the tested pesticides are highly toxic to the health of aquatic organisms and should be periodically and regularly monitored in the environment to reduce pollutant stress on non-target biota, particularly fish.

[1]  M. Azra,et al.  Cholinesterase activity as a potential biomarker for neurotoxicity induced by pesticides in vivo exposed Oreochromis niloticus (Nile tilapia): assessment tool for organophosphates and synthetic pyrethroids , 2021, Environmental technology.

[2]  M. Ikram,et al.  Sub-lethal effects of organophosphates and synthetic pyrethroid insecticides on muscle tissue transaminases of Oreochromis niloticus in vivo , 2021, Toxicological Research.

[3]  G. Nabi,et al.  Comparative acute toxicity of organophosphates and synthetic pyrethroid pesticides in vivo exposed fresh water fish Oreochromis niloticus (Linnaeus, 1758) , 2020, Aquatic Ecosystem Health and Management.

[4]  R. Majumder,et al.  Acute and sublethal effects of organophosphate insecticide chlorpyrifos on freshwater fish Oreochromis niloticus , 2019, Drug and chemical toxicology.

[5]  E. Suliman,et al.  Toxicity bioassay and sub-lethal effects of diazinon on blood profile and histology of liver, gills and kidney of catfish, Clarias gariepinus. , 2019, Brazilian journal of biology = Revista brasleira de biologia.

[6]  M. Kaur,et al.  Biomarker enzyme activities and ultrastructure of liver of the grass carp Ctenopharyngodon idella (Valenciennes, 1844) exposed to the organophosphate pesticide chlorpyrifos , 2017 .

[7]  S. Verma,et al.  Bioclinical stress of Rogor pesticide in the fish Amphipnous cuchia , 2016 .

[8]  M. Koohi,et al.  Permethrin-induced oxidative damage in liver of rainbow trout (Oncorhynchus mykiss) and its attenuation by vitamin C. , 2016, Iranian journal of veterinary research.

[9]  E. Shokr.  Effect of Ammonia Stress on Blood Constitutes in Nile Tilapia , 2015 .

[10]  E. Younis,et al.  Haematological and biochemical parameters and tissue accumulations of cadmium in Oreochromis niloticus exposed to various concentrations of cadmium chloride , 2015, Saudi Journal of Biological Sciences.

[11]  A. Rauf,et al.  Sub-lethal Effects of Diazinon on Hematological Indices and Blood Biochemical Parameters in Indian Carp, Cirrhinus mrigala (Hamilton) , 2014 .

[12]  A. Ghosh,et al.  Biochemical effects of glyphosate based herbicide, Excel Mera 71 on enzyme activities of acetylcholinesterase (AChE), lipid peroxidation (LPO), catalase (CAT), glutathione-S-transferase (GST) and protein content on teleostean fishes. , 2014, Ecotoxicology and environmental safety.

[13]  Amit Kumar,et al.  λ-Cyhalothrin and cypermethrin induce stress in the freshwater muddy fish, Clarias batrachus , 2014 .

[14]  I. Khan,et al.  In vitro antioxidant activities of four medicinal plants on the basis of DPPH free radical scavenging. , 2013, Pakistan journal of pharmaceutical sciences.

[15]  M. Banaee.  Physiological Dysfunction in Fish After Insecticides Exposure , 2013 .

[16]  T. Vani,et al.  Alteration in haematological and biochemical parameters of Catla catla exposed to sub-lethal concentration of cypermethrin , 2012, Fish Physiology and Biochemistry.

[17]  A. Kumar,et al.  Comparative study on the toxicity of pyrethroids, α-cypermethrin and deltamethrin to Ceriodaphnia dubia. , 2012, Ecotoxicology and environmental safety.

[18]  M. Ramesh,et al.  Carbamazepine (CBZ) induced enzymatic stress in gill, liver and muscle of a common carp, Cyprinus carpio , 2012 .

[19]  M. Banaee.  Adverse Effect of Insecticides on Various Aspects of Fish’s Biology and Physiology , 2012 .

[20]  T. Muralisankar,et al.  Effects of Quinalphos 25 EC and Dimethoate 30 EC on Activities of AchE, Catalase, GOT and GPT in the Freshwater Prawn Macrobrachium rosenbergii , 2011 .

[21]  Amit Kumar,et al.  Assessment of acute toxicity of λ-cyhalothrin to a freshwater catfish, Clarias batrachus , 2011 .

[22]  F. Jabeen,et al.  Assessing metal, protein, and DNA profiles in Labeo rohita from the Indus River in Mianwali, Pakistan , 2011, Environmental monitoring and assessment.

[23]  T. Cutright,et al.  Determination of pesticide residues in Turkey’s table grapes: the effect of integrated pest management, organic farming, and conventional farming , 2011, Environmental monitoring and assessment.

[24]  Özgür Fırat,et al.  A comparative study on the effects of a pesticide (cypermethrin) and two metals (copper, lead) to serum biochemistry of Nile tilapia, Oreochromis niloticus , 2011, Fish Physiology and Biochemistry.

[25]  M. Costache,et al.  Malathion‐induced alteration of the antioxidant defence system in kidney, gill, and intestine of Carassius auratus gibelio , 2009, Environmental toxicology.

[26]  Oe Ogueji.  Comparative Acute Toxicity Of Chlorpyrifos-Ethyl (Organophosphate) And Lambda-Cyhalothrin (Pyrethroid) To The African Catfish ( C. gariepinus ) Using Some Biochemical Parameters , 2008 .

[27]  Bernadette C Ossendorp,et al.  Cumulative risk assessment of pesticide residues in food. , 2008, Toxicology letters.

[28]  R McKinlay,et al.  Calculating human exposure to endocrine disrupting pesticides via agricultural and non-agricultural exposure routes. , 2008, The Science of the total environment.

[29]  S. Neelagund,et al.  Impact of Cypermethrin on enzyme activities in the freshwater fish Cirrhinus mrigala (Hamilton) , 2008 .

[30]  Krishna Gopal,et al.  Biochemical alteration induced by monocrotophos in the blood plasma of fish, Channa punctatus (Bloch) , 2007 .

[31]  J. Rao.  Sublethal effects of an organophosphorus insecticide (RPR-II) on biochemical parameters of tilapia, Oreochromis mossambicus. , 2006 .

[32]  G. Begum.  In vivo biochemical changes in liver and gill of Clarias batrachus during cypermethrin exposure and following cessation of exposure , 2005 .

[33]  I. Metón,et al.  The N‐terminal sequence directs import of mitochondrial alanine aminotransferase into mitochondria , 2004, FEBS letters.

[34]  J. L. Arredondo-Figueroa,et al.  Variability of tilapias (Oreochromis spp.) introduced in Mexico: morphometric, meristic and genetic characters , 2004 .

[35]  G. Begum.  Carbofuran insecticide induced biochemical alterations in liver and muscle tissues of the fish Clarias batrachus (linn) and recovery response. , 2004, Aquatic toxicology.

[36]  Edward V. Sargent,et al.  A Human Health Risk Assessment of Pharmaceuticals in the Aquatic Environment , 2002 .

[37]  V. Mehta,et al.  Toxicity study of diethyl phthalate on freshwater fish Cirrhina mrigala. , 2002, Ecotoxicology and environmental safety.

[38]  R. Helm,et al.  Evaluation of Immune Parameters in Propanil-Exposed Farm Families , 2001, Archives of environmental contamination and toxicology.

[39]  Susan B. Jones,et al.  Physiological measures of neurotoxicity of diazinon and malathion to larval rainbow trout (Oncorhynchus mykiss) and their correlation with behavioral measures , 2000 .

[40]  J. Galindo-Reyes,et al.  Enzymatic and osmoregulative alterations in white shrimp Litopenaeus vannamei exposed to pesticides. , 2000, Chemosphere.

[41]  P. Thangavel,et al.  Glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) enzyme activities in different tissues of Sarotherodon mossambicus (Peters) exposed to a carbamate pesticide, carbaryl , 1999 .

[42]  N. S. Oluah.  Plasma Aspartate Aminotransferase Activity in the Catfish Clarias albopunctatus Exposed to Sublethal Zinc and Mercury , 1999, Bulletin of environmental contamination and toxicology.

[43]  E. Oruç,et al.  Effects of 2,4-Diamin on some parameters of protein and carbohydrate metabolisms in the serum, muscle and liver of Cyprinus carpio , 1999 .

[44]  F. Dulout,et al.  Genotoxic evaluation of the pyrethroid lambda-cyhalothrin using the micronucleus test in erythrocytes of the fish Cheirodon interruptus interruptus. , 1999, Mutation research.

[45]  R. Carr,et al.  Selective toxicity of chlorpyrifos to several species of fish during an environmental exposure: Biochemical mechanisms , 1997 .

[46]  K. Halasy,et al.  Biochemical and subcellular changes in carp exposed to the organophosphorus methidathion and the pyrethroid deltamethrin , 1995 .

[47]  G. Chuiko,et al.  Feeding behavior and brain acetylcholinesterase activity in bream (Abramis brama L.) as affected by DDVP, an organophosphorus insecticide. , 1992, Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology.

[48]  M. Panteghini.  Aspartate aminotransferase isoenzymes. , 1990, Clinical biochemistry.

[49]  S. D. Murphy,et al.  Toxicology of Pesticides , 1987, NATO ASI Series.

[50]  M. Amin.  Effects of Pesticides on Total Protein Content of Different Organs of Oreochromis niloticus (Linnaeus, 1758) , 2021 .

[51]  El Miniawy.  Sublethal effects of the herbicide thiobencarb on fecundity, histopathological and biochemical changes in the African catfish (Clarias gariepinus) , 2018 .

[52]  P. Bhavan,et al.  Lethal and Sub-lethal Toxic effects of a Pyrethroid Insecticide, λ- Cyhalothrin on activities of Acetylcholinesterase, Glutamic Oxaloacetic Transaminase, Glutamic Pyruvic Transaminase and Catalase in the Post-larvae of the Prawn Macrobrachium rosenbergii , 2015 .

[53]  R. Sowdeswari,et al.  Acute Toxicity Of Heavy Metals (Cadmium Chloride, Chromium Trioxide And Lead Nitrate) And Their Effects On The Freshwater Prawn Macrobrachium Rosenbergii , 2012 .

[54]  R. State.  Changes in Metabolic Enzymes Activities in Selected Organs and Tissue of Clarias Gariepinus Exposed to Cypermethrin , 2012 .

[55]  A. Sureda,et al.  Effects of diazinon on biochemical parameters of blood in rainbow trout (Oncorhynchus mykiss) , 2011 .

[56]  M. Zaki,et al.  Biochemical, Clinicophathlogical And Microbial Changes In Clarias Gari epinus Exposed To Pesticide Malathion And Climate Change , 2010 .

[57]  F. Mohamed.  Histopathological Studies on Tilapia zillii and Solea vulgaris from Lake Qarun, Egypt , 2009 .

[58]  S. Singh,et al.  EFFECT OF INDOFIL TOXICITY ON MCHC OF Channa punctatus (BLOCH.) , 2007 .

[59]  G. Venkataramana,et al.  Impact of malathion on the biochemical parameters of gobiid fish, Glossogobius giuris (Ham). , 2006, Journal of environmental biology.

[60]  B. Das,et al.  The immunosuppressive effect of α-permethrin on Indian major carp, rohu (Labeo rohita Ham.) , 2004 .

[61]  M. Saier,et al.  Alanine Aminotransferase , 2003 .

[62]  Sac-fry Stages,et al.  OECD GUIDELINE FOR TESTING OF CHEMICALS , 2002 .

[63]  H. Okabe.  [Aspartate aminotransferase]. , 1995, Nihon rinsho. Japanese journal of clinical medicine.

[64]  S. Bhattacharya.  Target and non-target effects of anticholinesterase pesticides in fish , 1993 .

[65]  Y. Takimoto,et al.  The fate of pesticides in aquatic ecosystems. , 1990 .

[66]  J. Bloomquist,et al.  Neurotoxic actions of pyrethroid insecticides. , 1989, Annual review of entomology.

[67]  L. Costa.  Toxicology of Pesticides: A Brief History , 1987 .