Live-cell Imaging of filamentous fungi using vital fluorescent dyes and confocal microscopy

Publisher Summary This chapter discusses microscope technologies for imaging living fungal cells at high spatial resolution and reviews the vital fluorescent dyes that are proving useful for analyzing the cell biology of filamentous fungi with the confocal laser-scanning microscope (CLSM). Definitions of important practical aspects that need to be taken into account for optimal live-cell imaging at high spatial resolution with the CLSM and indications of future directions for live-cell imaging of filamentous fungi are described in the chapter. A range of microscope technologies have been developed over the past 20 years that allow the imaging of living cells at high spatial resolution using optical sectioning. These technologies include CLSM , spinning-disk confocal microscopy, two-photon microscopy, and deconvolution microscopy. The advantages and disadvantages of these optical-sectioning methods for imaging living fungal cells are summarized in the chapter. Of these types of microscopies, CLSM has been the most popular, and this chapter focuses on results obtained using this extremely powerful imaging technique.

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