Potential of antimicrobial active packaging ‘containing natamycin, nisin, pomegranate and grape seed extract in chitosan coating’ to extend shelf life of fresh strawberry

Abstract In this study, chitosan (CH) coatings as carrier of nisin (NS), natamycin (NT), pomegranate (PE) and grape seed extract (GE) were used to maintain quality and extend shelf life of fresh strawberry. The samples were analyzed in terms of gas composition inside packaging, pH, total soluble solid content, water activity, color, texture, FT-NIR and microbial counts for up to 40 days at 4 C. The experiments showed that all coating materials preserved the quality of fresh strawberry compared to untreated ones. Among the treatments, incorporation of the natamycin in the chitosan coating (CH + NT) reduced the O 2 consumption of the fruit and showed better effects on delaying changes of pH, total soluble solid content, water activity and microbial counts. The incorporation of different antimicrobial agents into chitosan matrix did not reveal any significant effect on lightness ( L *) and redness ( a *) of strawberry. Among the treatments, CH + PE and CH + NT showed significantly higher antimicrobial effect against the mesophilic bacteria and yeast and mold. As a general result, antimicrobial active packaging based on the combination of chitosan coating with antimicrobial agents increased the shelf life of fresh strawberry compared to uncoated fruit.

[1]  I. Sousa,et al.  Control of pathogenic and spoilage microorganisms from cheese surface by whey protein films containing malic acid, nisin and natamycin , 2010 .

[2]  W. F. Harrigan Laboratory Methods in Food Microbiology , 1998 .

[3]  B. Alsanius,et al.  Improving Strawberry Fruit Storability by Edible Coating as a Carrier of Thymol or Calcium Chloride , 2010 .

[4]  A. Vega‐Gálvez,et al.  Osmotic dehydration under high hydrostatic pressure: Effects on antioxidant activity, total phenolics compounds, vitamin C and colour of strawberry (Fragaria vesca) , 2013 .

[5]  M. G. Johnson,et al.  Evaluation of antibacterial activity of whey protein isolate coating incorporated with nisin, grape seed extract, malic acid, and EDTA on a Turkey frankfurter system. , 2008, Journal of food science.

[6]  J. Rhoades,et al.  Antimicrobial Actions of Degraded and Native Chitosan against Spoilage Organisms in Laboratory Media and Foods , 2000, Applied and Environmental Microbiology.

[7]  A. Deschamps,et al.  Antimicrobial edible packaging based on cellulosic ethers, fatty acids, and nisin incorporation to inhibit Listeria innocua and Staphylococcus aureus. , 2001, Journal of food protection.

[8]  C. Genevois,et al.  Effect of iron and ascorbic acid addition on dry infusion process and final color of pumpkin tissue , 2014 .

[9]  L. Franssen 12 – Edible coatings containing natural antimicrobials for processed foods , 2003 .

[10]  D. Kang,et al.  Fate of biofilm cells of Cronobacter sakazakii under modified atmosphere conditions , 2014 .

[11]  Yuyue Qin,et al.  Characterization of Antioxidant Chitosan Film Incorporated with Pomegranate Peel Extract , 2013 .

[12]  António A. Vicente,et al.  Evaluation of a chitosan-based edible film as carrier of natamycin to improve the storability of Saloio cheese , 2010 .

[13]  Tadapaneni Venkata Ramana Rao,et al.  Improvement of quality and shelf-life of strawberries with edible coatings enriched with chitosan , 2013 .

[14]  Michael G. Johnson,et al.  Control of Listeria monocytogenes on the Surface of Refrigerated, Ready-to-eat Chicken Coated with Edible Zein Film Coatings Containing Nisin and/or Calcium Propionate , 2002 .

[15]  G. Tsai,et al.  Antibacterial activity of shrimp chitosan against Escherichia coli. , 1999, Journal of food protection.

[16]  Amparo Chiralt,et al.  Effect of chitosan–lemon essential oil coatings on storage-keeping quality of strawberry , 2012 .

[17]  J. Tomasik,et al.  Natamycin In Ripening Cheeses , 2002 .

[18]  M. Poletto,et al.  Microbial inactivation kinetics during high-pressure carbon dioxide treatment: nonlinear model for the combined effect of temperature and pressure in apple juice. , 2008, Journal of food science.

[19]  M. Tapia,et al.  Use of alginate- and gellan-based coatings for improving barrier, texture and nutritional properties of fresh-cut papaya , 2008 .

[20]  M. S. Aday,et al.  The shelf life extension of fresh strawberries using an oxygen absorber in the biobased package , 2013 .

[21]  Eleonora Winkelhausen,et al.  Impact of chitosan-beeswax edible coatings on the quality of fresh strawberries (Fragaria ananassa cv Camarosa) under commercial storage conditions , 2013 .

[22]  Fengsong Cong,et al.  Use of surface coatings with natamycin to improve the storability of Hami melon at ambient temperature , 2007 .

[23]  Peter M.A. Toivonen,et al.  Biochemical bases of appearance and texture changes in fresh-cut fruit and vegetables , 2008 .

[24]  Ernestina Casiraghi,et al.  Evaluation of shelf-life of fresh-cut pineapple using FT-NIR and FT-IR spectroscopy , 2009 .

[25]  Michael G. Johnson,et al.  Inhibition of Listeria monocytogenes by Nisin Combined with Grape Seed Extract or Green Tea Extract in Soy Protein Film Coated on Turkey Frankfurters , 2006 .

[26]  Amparo Chiralt,et al.  Quality of cold-stored strawberries as affected by chitosan–oleic acid edible coatings , 2006 .

[27]  Vilas M. Salokhe,et al.  Enhancing antimicrobial activity of chitosan films by incorporating garlic oil, potassium sorbate and nisin , 2005 .

[28]  I. Savvaidis,et al.  Efficacy of nisin and/or natamycin to improve the shelf-life of Galotyri cheese. , 2013, Food microbiology.

[29]  F. Malcata,et al.  Evaluation of antimicrobial edible coatings from a whey protein isolate base to improve the shelf life of cheese. , 2012, Journal of dairy science.

[30]  Scott W. Leonard,et al.  Edible coatings to improve storability and enhance nutritional value of fresh and frozen strawberries (Fragaria × ananassa) and raspberries (Rubus ideaus) , 2004 .

[31]  Adel A. Kader,et al.  Controlled atmosphere-induced changes in pH and organic acid metabolism may affect color of stored strawberry fruit , 1999 .

[32]  Frank Devlieghere,et al.  Chitosan: antimicrobial activity, interactions with food components and applicability as a coating on fruit and vegetables , 2004 .

[33]  Huaning Yu,et al.  Characterization and antimicrobial properties of water chestnut starch-chitosan edible films. , 2013, International journal of biological macromolecules.

[34]  Adriano Brandelli,et al.  Antimicrobial activity of chitosan films containing nisin, peptide P34, and natamycin , 2012 .

[35]  L. Palou,et al.  Antimicrobial Edible Films and Coatings for Fresh and Minimally Processed Fruits and Vegetables: A Review , 2011, Critical reviews in food science and nutrition.

[36]  F. Shahidi,et al.  Food applications of chitin and chitosans , 1999 .

[37]  M. S. Aday,et al.  Understanding the effects of various edible coatings on the storability of fresh cherry , 2010 .

[38]  Jasmine Y Serpen Comparison of Sugar Content in Bottled 100% Fruit Juice versus Extracted Juice of Fresh Fruit , 2012 .

[39]  R. Toledo,et al.  Health aspects of functional grape seed constituents , 2004 .

[40]  R. C. Wiley,et al.  Minimally Processed Refrigerated Fruits & Vegetables , 2012 .

[41]  S. Burapadaja,et al.  Antimicrobial activity of tannins from Terminalia citrina. , 1995, Planta medica.

[42]  Cengiz Caner,et al.  Effect of oxygen and carbon dioxide absorbers on strawberry quality , 2011 .

[43]  Stefania Quintavalla,et al.  Antimicrobial food packaging in meat industry. , 2002, Meat science.

[44]  M. Hendrickx,et al.  Enzyme infusion prior to thermal/high pressure processing of strawberries: Mechanistic insight into firmness evolution , 2010 .

[45]  W. Du,et al.  Increasing strawberry shelf-life with carvacrol and methyl cinnamate antimicrobial vapors released from edible films , 2014 .

[46]  Michael G. Johnson,et al.  Physical and Chemical Properties of Edible Films Containing Nisin and Their Action Against Listeria Monocytogenes , 2001 .

[47]  Michael G. Johnson,et al.  Physical and antimicrobial properties of grape seed extract, nisin, and EDTA incorporated soy protein edible films , 2008 .

[48]  E. Ryser,et al.  Antimicrobial edible films and coatings. , 2004, Journal of food protection.

[49]  M. Martínez-Ferrer,et al.  Modified Atmosphere Packaging of Minimally Processed Mango and Pineapple Fruits , 2002 .

[50]  M. Moradi,et al.  Characterization of antioxidant chitosan film incorporated with Zataria multiflora Boiss essential oil and grape seed extract , 2012 .

[51]  M. Romero,et al.  Relationships Between Sensory Flavor Evaluation and Volatile and Nonvolatile Compounds in Commercial Wheat Bread Type Baguette , 2006 .

[52]  Mir Naiman Ali,et al.  Studies on Antibacterial and Antifungal Activity of Pomegranate (Punica granatum L.) , 2010 .

[53]  Cengiz Caner,et al.  An innovative technique for extending shelf life of strawberry: Ultrasound , 2013 .

[54]  K. Song,et al.  Preparation of a barley bran protein–gelatin composite film containing grapefruit seed extract and its application in salmon packaging , 2012 .

[55]  S. Bañón,et al.  Ascorbate, green tea and grape seed extracts increase the shelf life of low sulphite beef patties. , 2007, Meat science.

[56]  Arun Sharma,et al.  Antioxidant potential of mint (Mentha spicata L.) in radiation-processed lamb meat , 2007 .

[57]  Cengiz Caner,et al.  Use of microperforated films and oxygen scavengers to maintain storage stability of fresh strawberries , 2012 .

[58]  J. Delves-Broughton Nisin and its application as a food preservative. , 1990 .

[59]  Lifang Feng,et al.  Physicochemical responses and microbial characteristics of shiitake mushroom (Lentinus edodes) to gum arabic coating enriched with natamycin during storage. , 2013, Food chemistry.