A Study on BMPR-IB Genes of Bayanbulak Sheep

The average twin lambing rate of Bayanbulak sheep is 2% to 3%. However, a flock of sheep with a close genetic relationship and an average of 2 to 3 lambs per birth has been found recently. To determine the major genes controlling the prolificacy of the flock in the present study, the flock was designated A while 100 normal Bayanbulak sheep were randomly selected to comprise the control flock B. Ligase detection reaction method was applied to detect and analyze the 10 mutational loci of the 3 candidate prolificacy genes including bone morphogenetic protein type I receptors, bone morphogenetic protein 15, and growth differentiation factor 9. The 10 mutational loci are as follows: FecB locus of the BMPR-IB gene; FecXI, FecXB, FecXL, FecXH, FecXG, and FecXR of the BMP15 gene; and G1, G8, and FecTT of the GDF9 gene. Two mutations including BMPR-IB/FecB and GDF9/G1 were found in Bayanbulak sheep. Independence test results of the two flocks demonstrate that the FecB locus has a significant effect on the lambing number of Bayanbulak sheep. However, the mutation frequency of the G1 locus in GDF9 is very low. Independence test results demonstrate that the GDF9 locus does not have a significant impact on the lambing performance of Bayanbulak sheep. Among the 10 detected loci, BMPR-IB/FecB is the major gene that influences the high lambing rate of Bayanbulak sheep.

[1]  L. Jia,et al.  Polymorphisms of coding region of BMPR-IB gene and their relationship with litter size in sheep , 2011, Molecular Biology Reports.

[2]  S. De,et al.  Polymorphism of BMPR1B, BMP15 and GDF9 fecundity genes in prolific Garole sheep , 2010, Tropical Animal Health and Production.

[3]  R. Pong-Wong,et al.  Homozygosity for a single base-pair mutation in the oocyte-specific GDF9 gene results in sterility in Thoka sheep. , 2009, Reproduction.

[4]  R. Kaushik,et al.  Polymorphism of fecundity genes (BMPR1B, BMP15 and GDF9) in the Indian prolific Black Bengal goat , 2009 .

[5]  A. Laviña,et al.  A 17 bp deletion in the Bone Morphogenetic Protein 15 (BMP15) gene is associated to increased prolificacy in the Rasa Aragonesa sheep breed. , 2009, Animal reproduction science.

[6]  J. Calvo,et al.  A deletion in the bone morphogenetic protein 15 gene causes sterility and increased prolificacy in Rasa Aragonesa sheep. , 2008, Animal genetics.

[7]  M. Chu,et al.  Mutations in BMPR-IB and BMP-15 genes are associated with litter size in Small Tailed Han sheep (Ovis aries). , 2007, Journal of animal science.

[8]  P. Monget,et al.  A novel mutation in the bone morphogenetic protein 15 gene causing defective protein secretion is associated with both increased ovulation rate and sterility in Lacaune sheep. , 2007, Endocrinology.

[9]  P. Monget,et al.  Reproductive Biology and Endocrinology Regulation of Ovulation Rate in Mammals: Contribution of Sheep Genetic Models , 2022 .

[10]  N. Cockett,et al.  Investigation of the Booroola (FecB) and Inverdale (FecX(I)) mutations in 21 prolific breeds and strains of sheep sampled in 13 countries. , 2006, Animal reproduction science.

[11]  S. Shimasaki,et al.  Molecular biology and physiological role of the oocyte factor, BMP-15 , 2005, Molecular and Cellular Endocrinology.

[12]  M. Mullen,et al.  Mutations in the Genes for Oocyte-Derived Growth Factors GDF9 and BMP15 Are Associated with Both Increased Ovulation Rate and Sterility in Cambridge and Belclare Sheep (Ovis aries)1 , 2004, Biology of reproduction.

[13]  L. Du,et al.  [Studies of BMPR-IB and BMP15 as candidate genes for fecundity in little tailed han sheep]. , 2003, Yi chuan xue bao = Acta genetica Sinica.

[14]  Du Li.  Studies on Microsatellite and RAPD Markers in Little-tailed Han Sheep , 2003 .

[15]  J. Juengel,et al.  Growth Differentiation Factor 9 and Bone Morphogenetic Protein 15 Are Essential for Ovarian Follicular Development in Sheep1 , 2002, Biology of reproduction.

[16]  M. Sancristobal,et al.  Segregation of a major gene influencing ovulation in progeny of Lacaune meat sheep , 2002, Genetics Selection Evolution.

[17]  Subandriyo,et al.  DNA Tests in Prolific Sheep from Eight Countries Provide New Evidence on Origin of the Booroola (FecB) Mutation1 , 2002, Biology of reproduction.

[18]  P. Monget,et al.  Mutation in bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booroola Mérino ewes , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[19]  Shin Yamamoto,et al.  Bone Morphogenetic Protein-15 Inhibits Follicle-stimulating Hormone (FSH) Action by Suppressing FSH Receptor Expression* , 2001, The Journal of Biological Chemistry.

[20]  G. Montgomery,et al.  Highly Prolific Booroola Sheep Have a Mutation in the Intracellular Kinase Domain of Bone Morphogenetic Protein IB Receptor (ALK-6) That Is Expressed in Both Oocytes and Granulosa Cells1 , 2001, Biology of reproduction.

[21]  Shin Yamamoto,et al.  Bone Morphogenetic Protein-15 , 2000, The Journal of Biological Chemistry.

[22]  G. Montgomery,et al.  Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage-sensitive manner , 2000, Nature Genetics.

[23]  Francis Barany,et al.  Improving the fidelity of Thermus thermophilus DNA ligase , 1996 .

[24]  F. Barany,et al.  Improving the fidelity of Thermus thermophilus DNA ligase. , 1996, Nucleic acids research.