Comparison of the low level laser therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence *

The low level laser therapy (LLLT) has been used in Dentistry to improve wound healing. In order to analyse the effect of LLLT on the in vitro proliferation of gingival fibroblasts we developed a primary culture of human gingival fibroblasts.

[1]  Chukuka S. Enwemeka,et al.  Morphometrics of Collagen Fibril Populations in He:Ne Laser Photostimulated Tendons , 1990 .

[2]  R. Patrick Abergel,et al.  Increased Wound-Healing Rate In Pig Skin Treated By Helium-Neon Laser. , 1988, Photonics West - Lasers and Applications in Science and Engineering.

[3]  M. Dyson,et al.  Stimulatory effect of 660 nm low level laser energy on hypertrophic scar‐derived fibroblasts: possible mechanisms for increase in cell counts , 1998, Lasers in surgery and medicine.

[4]  Henrik Loevschall,et al.  Effect of low level diode laser irradiation of human oral mucosa fibroblasts in vitro , 1994, Lasers in surgery and medicine.

[5]  J C Franquin,et al.  Helium-neon laser treatment transforms fibroblasts into myofibroblasts. , 1990, The American journal of pathology.

[6]  • Epidermis,et al.  WOUND healing. , 1959, The Medical journal of Australia.

[7]  Á. Mester,et al.  The biostimulative effect of low level laser therapy of long-standing crural ulcers using helium neon laser, helium neon plus infrared lasers, and noncoherent light: Preliminary report of a randomized double blind comparative study , 1989 .

[8]  M. Domingues,et al.  A novel cell line that retains the morphological characteristics of the cells and matrix of odontogenic myxoma. , 2000, Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology.

[9]  J P Gage,et al.  A preliminary study of the effects of laser radiation on collagen metabolism in cell culture. , 1996, Australian dental journal.

[10]  J. Basford,et al.  Does low‐energy helium‐neon laser irradiation alter “in vitro” replication of human fibroblasts? , 1988, Lasers in surgery and medicine.

[11]  R J Lanzafame,et al.  THE EFFECT OF LASER IRRADIATION ON THE RELEASE OF bFGF FROM 3T3 FIBROBLASTS , 1994, Photochemistry and photobiology.

[12]  J. Uitto,et al.  Cellular effects of the pulsed tunable dye laser at 577 nanometers on human endothelial cells, fibroblasts, and erythrocytes: An in vitro study , 1988, Lasers in surgery and medicine.

[13]  R Lubart,et al.  Effects of visible and near-infrared lasers on cell cultures. , 1992, Journal of photochemistry and photobiology. B, Biology.

[14]  Semion Rochkind,et al.  Systemic effects of low‐power laser irradiation on the peripheral and central nervous system, cutaneous wounds, and burns , 1989, Lasers in surgery and medicine.

[15]  M. Dyson,et al.  EFFECT OF LIGHT ON CALCIUM UPTAKE BY MACROPHAGES , 1990 .

[16]  G. Schmalz Use of cell cultures for toxicity testing of dental materials--advantages and limitations. , 1994, Journal of dentistry.

[17]  J. Uitto,et al.  Assay of collagenase activity by a rapid, sensitive, and specific method. , 1982, Collagen and related research.

[18]  A. Emons,et al.  Boekbespreking: Molecular biology of the cell, B. Alberts, D. Bray, J. Lewis, M. Raff, K. Robers, D.J. Watson. Garland Publ., New York. 1989. , 1990 .

[19]  W. J. Langford Statistical Methods , 1959, Nature.

[20]  T. S. Lam Laser stimulation of collagen synthesis in human skin fibroblast cultures , 1986 .

[21]  J. C. Gómez-Villamandos,et al.  HeNe laser therapy by fibroendoscopy in the mucosa of the equine upper airway , 1995, Lasers in Surgery and Medicine.

[22]  Karu Ti,et al.  Long-term and short-term responses of human lymphocytes to He-Ne laser radiation , 1991 .

[23]  M. Dyson,et al.  THE EFFECT OF POLARIZED LIGHT ON THE RELEASE OF GROWTH FACTORS FROM THE U-937 MACROPHAGE-LIKE CELL LINE , 1992 .

[24]  A. Watban,et al.  STIMULATIVE AND INHIBITORY EFFECTS OF LOW INCIDENT LEVELS OF ARGON LASER ENERGY ON WOUND HEALING , 1995 .

[25]  M. Dyson,et al.  Macrophage responsiveness to light therapy , 1989, Lasers in surgery and medicine.

[26]  Hans H. F. I. van Breugel,et al.  Power density and exposure time of He‐Ne laser irradiation are more important than total energy dose in photo‐biomodulation of human fibroblasts in vitro , 1992, Lasers in surgery and medicine.

[27]  M. Soudry,et al.  Modifications of growth dynamics and ultrastructure after helium-neon laser treatment of human gingival fibroblasts. , 1989, Quintessence international.

[28]  M. Moreno-Millán,et al.  Helium-Neon (He-Ne) Laser Irradiation Increases the Incidence of Unreduced Bovine Oocytes During the First Meiotic Division In Vitro , 1998, Lasers in Medical Science.

[29]  R. Jaeger,et al.  Effect of a ceramic and a non-ceramic hydroxyapatite on cell growth and procollagen synthesis of cultured human gingival fibroblasts. , 2000, Journal of periodontology.

[30]  Nili Grossman,et al.  BIOSTIMULATION OF PHOTOSENSITIZED FIBROBLASTS BY LOW INCIDENT LEVELS OF VISIBLE LIGHT ENERGY , 1995 .

[31]  J. Davies,et al.  Molecular Biology of the Cell , 1983, Bristol Medico-Chirurgical Journal.

[32]  T. Karu DEREPRESSION OF THE GENOME AFTER IRRADIATION OF HUMAN LYMPHOCYTES WITH HE-NE LASER , 1992 .

[33]  M. Trelles,et al.  THE ACTION OF LOW REACTIVE LEVEL LASER THERAPY (LLLT) ON MAST CELLS , 1989 .

[34]  A. Mester,et al.  Mester’s Method of Laser Biostimulation , 1986 .

[35]  C. Kevin,et al.  A DOUBLE BLIND CROSSOVER TRAIALOF LOW LEVEL LASER THERAPY IN THE TREATMENT OF POST HERPETIC NEURALGIA , 1989 .

[36]  U. Covani,et al.  Periodontal tissue regeneration in beagle dogs after laser therapy , 1997, Lasers in surgery and medicine.

[37]  M. Dyson,et al.  MACROPHAGE RESPONSIVENESS TO LIGHT THERAPY WITH VARYING POWER AND ENERGY DENSITIES , 1991 .

[38]  D. Hirst,et al.  Effect of low intensity monochromatic light therapy (890 nm) on a radiation‐impaired, wound‐healing model in murine skin , 1998, Lasers in surgery and medicine.

[39]  Mary Dyson,et al.  THE EFFECTS OF LOW LEVEL LASER THERAPY ON THE PROLIFERATION OF KERATINOCYTES , 1993 .

[40]  J C Franquin,et al.  [Modification of growth dynamics and ultrastructure of human gingival fibroblasts after treatment with an helium-neon-laser]. , 1989, Die Quintessenz.

[41]  K. Skogerboe,et al.  Laser photobioactivation mechanisms: In vitro studies using ascorbic acid uptake and hydroxyproline formation as biochemical markers of irradiation response , 1990, Lasers in surgery and medicine.

[42]  M. Dyson,et al.  THE DIRECT EFFECT OF 860 nm LIGHT ON CELL PROLIFERATION AND ON SUCCINIC DEHYDROGENASE ACTIVITY OF HUMAN FIBROBLASTS INVITRO , 1995 .

[43]  A. Kruse,et al.  Cytokine production after helium-neon laser irradiation in cultures of human peripheral blood mononuclear cells. , 1992, Journal of photochemistry and photobiology. B, Biology.

[44]  G Godlewski,et al.  Morphologic changes in collagen fibers after 830 nm diode laser welding , 1997, Lasers in surgery and medicine.

[45]  Nili Grossman,et al.  LIGHT EFFECT ON FIBROBLAST PROLIFERATON , 1993 .