Spawning induction in Kutum, Rutilus frisii kutum (Kamensky), with different hormones: Analysis of hormone profiles and induced spawning success

Abstract The objective of this study was to evaluate the effectiveness of different hormones on spawning success and sex steroid changes in Caspian Kutum, Rutilus frisii kutum. Groups of five fish were injected intramuscularly as follows: 2 mg kg-1 common carp pituitary extract (CPE) as the positive control, 100 IU kg-1 human chorionic gonadotropin (hCG), 2 μg kg-1 luteinizing hormone releasing hormone analog (LHRHa2), 10 μg kg-1 synthetic analogue gonadoliberins (sGnRHa), 0.2 mL kg-1 Ovaprim which is a complex of salmon GnRH analogue with a dopamine receptor antagonist (domperidone), a combination of 2 μg kg-1 LHRHa2 and 100 IU kg-1 hCG, a complex of 10 μg kg-1 sGnRHa and 100 hCG IU kg-1, and saline solution as the negative control. Blood samples were taken simultaneously with injections and after ovulation. The results showed that no fish ovulated in the negative control group. In the CPE treatment group, 4 of 5 fish ovulated. In the other experimental groups; no response was observed in the hCG group, while the females injected with sGnRHa-alone, LHRHa2-alone, and sGnRH+hCG had low spawning percentages. Of the hormones tested, the combination of sGnRHa+domperidone was the most effective spawning inducer and the greatest spawning success was obtained. Significant decreases after injection were observed in plasma concentrations of 17α estradiol, 17β-hydroxy progesterone, and progesterone in all treatments, while plasma testosterone concentrations increased significantly. This study revealed that a combined sGnRHa and dopamine antagonist can improve the effectiveness of artificial reproduction of Kutum. Since the combination of sGnRHa and domperidone is purer than CPE and has the most effective in inducing ovulation, it is preferred for inducing spawning in this species.

[1]  A. Lejk,et al.  Impact of selected hormonal agents on the effectiveness of controlled reproduction of cultivated female European grayling , 2012 .

[2]  S. Sharaf.  Effect of GnRHa, pimozide and Ovaprim on ovulation and plasma sex steroid hormones in African catfish Clarias gariepinus. , 2012, Theriogenology.

[3]  S. Oryan,et al.  Effects of LHRH-A2 and chlorpromazine (dopamine antagonists) on inducing spawning in Caspian Kutum, Rutilus frisii kutum, from the southwest of the Caspian Sea , 2012 .

[4]  A. Mamcarz,et al.  OPTIMIZATION OF ARTIFICIAL REPRODUCTION OF ASP, ASPIUS ASPIUS (L.) UNDER CONTROLLED CONDITIONS , 2011 .

[5]  A. Mamcarz,et al.  Artificial spawning of common tench Tinca tinca (Linnaeus, 1758), obtained from wild and domestic stocks , 2011, Aquaculture International.

[6]  V. Gracia-López,et al.  Comparison of the Effects of HCG and LHRHa on the Induction of Ovulation of Wild and Captive Leopard Grouper, Mycteroperca rosacea , 2010 .

[7]  A. Mamcarz,et al.  Controlled reproduction of asp, Aspius aspius (L.) using luteinizing hormone releasing hormone (LHRH) analogues with dopamine inhibitors , 2010 .

[8]  A. Arukwe,et al.  Sex steroid levels, oocyte maturation and spawning performance in Waigieu seaperch (Psammoperca waigiensis) exposed to thyroxin, human chorionic gonadotropin, luteinizing hormone releasing hormone and carp pituitary extract. , 2010, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.

[9]  B. Heidari,et al.  Changes in plasma levels of steroid hormones during oocyte development of Caspian Kutum (Rutilus frisii kutum, Kamensky, 1901). , 2010 .

[10]  H. Hashimoto,et al.  Effects of continuous administration of human chorionic gonadotropin, salmon pituitary extract, and gonadotropin-releasing hormone using osmotic pumps on induction of sexual maturation in male Japanese eel, Anguilla japonica , 2009 .

[11]  S. Cheung,et al.  Effects of GnRHa (D-Ala6, Pro9-NEt) combined with domperidone on ovulation induction in wild loach Misgurnus anguillicaudatus , 2009 .

[12]  A. Mamcarz,et al.  Artificial off-season spawning of Eurasian perch Perca fluviatilis L. , 2009 .

[13]  S. Gorgin,et al.  Study on sexual maturity and levels of gonad steroid hormones in female kutum Rutilus frisii kutum (Kamenskii, 1901) during spawning season from river Sefid-Rood of the southern Caspian sea , 2009 .

[14]  A. Mamcarz,et al.  Reproduction of chub, Leuciscus cephalus L., under controlled conditions , 2008 .

[15]  L. Hanson,et al.  Effect of Carp Pituitary Extract and Luteinizing Hormone Releasing Analog Hormone on Reproductive Indices and Spawning of 3-Year-Old Channel Catfish , 2008 .

[16]  D. Berlinsky,et al.  A comparison of human chorionic gonadotropin and luteinizing hormone releasing hormone analogue for ovulation induction in black sea bass Centropristis striata (Linnaeus, 1758) , 2007 .

[17]  F. P. Heyrati,et al.  Induced spawning of kutum, Rutilus frisii kutum (Kamenskii, 1901) using (D-Ala6, Pro9-NEt) GnRHa combined with domperidone , 2007 .

[18]  A. Behrouzi,et al.  THE INVESTIGATION OF STATUS BROOD STOCKS AND RELEASING FINGERLINGS OF RUTILUS FRISII KUTUM (KAMINSKI, 1901) IN THE SOUTH OF CASPIAN SEA (MAZANDARAN PROVINCE, 2004) , 2007 .

[19]  G. Martínez‐Rodríguez,et al.  Induction of spawning of captive-reared Senegal sole (Solea senegalensis) using different administration methods for gonadotropin-releasing hormone agonist , 2006 .

[20]  F. P. Heyrati,et al.  Spawning induction in Kutum Rutilus frisii kutum (Kamenskii, 1901) using carp pituitary extract or GnRH analogue combined with metoclopramide , 2006 .

[21]  E. Brzuska.  Artificial spawning of carp (Cyprinus carpio L.): differences between females of Polish strain 6 and Hungarian strain W treated with carp pituitary homogenate, Ovopel or Dagin , 2005 .

[22]  B. Levavi-Sivan,et al.  Enhancing spawning in the grey mullet (Mugil cephalus) by removal of dopaminergic inhibition. , 2005, General and comparative endocrinology.

[23]  G. Kraak,et al.  Dopamine inhibits gonadotropin secretion in the Chinese loach (Paramisgurnus dabryanus) , 1989, Fish Physiology and Biochemistry.

[24]  J. García-Alonso,et al.  Induction of oocyte maturation in the white croaker Micropogonias furnieri (Pisces: Sciaenidae) by human chorionic gonadotropin. , 2004, Brazilian journal of biology = Revista brasleira de biologia.

[25]  B. Levavi-Sivan,et al.  Spawning induction and hormonal levels during final oocyte maturation in the silver perch (Bidyanus bidyanus) , 2004 .

[26]  Muhammed Arabacı,et al.  Induction of ovulation in ornamental common carp (Koi, Cyprinus carpio L.) using LHRHa ([d‐Ser(tBu)6, Pro9‐NEt]‐LHRH) combined with haloperidol and carp pituitary extract , 2004 .

[27]  T. Szabó,et al.  Ovulation induction in nase (Chondrostoma nasus, Cyprinidae) using pituitary extract or GnRH analogue combined with domperidone , 2002 .

[28]  Y. Zohar,et al.  Endocrine regulation and artificial induction of oocyte maturation and spermiation in basses of the genus Morone , 2001 .

[29]  Y. Zohar,et al.  Endocrine manipulations of spawning in cultured fish: from hormones to genes , 2001 .

[30]  J. Rinchard,et al.  Sex steroids in plasma of lake whitefish Coregonus clupeaformis during spawning in Lake Erie. , 2001, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP.

[31]  N. Pankhurst,et al.  The efficacy of exogenous hormones in stimulating changes in plasma steroids and ovulation in wild black bream Acanthopagrus butcheri is improved by treatment at capture , 2000 .

[32]  T. Watanabe,et al.  Effects of acute stress on plasma cortisol, sex steroid hormone and glucose levels in male and female sockeye salmon during the breeding season , 1999 .

[33]  A. Skrzypczak,et al.  Induced spawning in perch, Perca fluviatilis L., using FSH + LH with pimozide or metoclopramide , 1998 .

[34]  N. W. Pankhurst,et al.  In vitro steroid production by isolated ovarian follicles of the striped trumpeter , 1997 .

[35]  A. Skrzypczak,et al.  Induced spawning in perch, Perca fluviatilis L., using carp pituitary extract and HCG. , 1996 .

[36]  B. Richardson,et al.  Induced Spawning of Wild American Shad Alosa sapidissima Using Sustained Administration of Gonadotropin-Releasing Hormone Analog (GnRHa) , 1995 .

[37]  B. Levavi-Sivan,et al.  Possible sites of dopaminergic inhibition of gonadotropin release from the pituitary of a teleost fish, tilapia , 1995, Molecular and Cellular Endocrinology.

[38]  Z. Yaron.  Endocrine control of gametogenesis and spawning induction in the carp , 1995 .

[39]  B. Levavi-Sivan,et al.  Spawning induction in common carp (Cyprinus carpio) using pituitary extract or GnRH superactive analogue combined with metoclopramide: analysis of hormone profile, progress of oocyte maturation and dependence on temperature , 1994 .

[40]  G. Kraak,et al.  Effects of gonadotropin-releasing hormone agonists and dopamine antagonists on gonadotropin secretion and ovulation in Chinese loach, Paramisgurnus dabryanus , 1991 .

[41]  Y. Zohar,et al.  Degradation of gonadotropin-releasing hormones in the gilthead seabream, Sparus aurata. II. Cleavage of native salmon GnRH, mammalian LHRH, and their analogs in the pituitary, kidney, and liver. , 1990, General and comparative endocrinology.

[42]  Y. Nagahama,et al.  Developmental changes in steroidogenic responses of ovarian follicles of amago salmon (Oncorhynchus rhodurus) to chum salmon gonadotropin during oogenesis. , 1988, General and comparative endocrinology.

[43]  S. Dufour,et al.  Stimulation of gonadotropin release and of ovarian development, by the administration of a gonadoliberin agonist and of dopamine antagonists, in female silver eel pretreated with estradiol. , 1988, General and comparative endocrinology.

[44]  K. Aida,et al.  Hormone changes during ovulation and effects of steroid hormones on plasma gonadotropin levels and ovulation in goldfish. , 1987, General and comparative endocrinology.

[45]  P. V. Oordt,et al.  The dopaminergic inhibition of the gonadotropin-releasing hormone-induced gonadotropin release: an in vitro study with fragments and cell suspensions from pituitaries of the African catfish, Clarias gariepinus (Burchell). , 1986, General and comparative endocrinology.

[46]  G. Hunter,et al.  7 Induced Final Maturation, Ovulation, and Spermiation in Cultured Fish , 1983 .