Impedance immunosensor based on receptor protein adsorbed directly on porous gold film

Abstract In this paper, a new immunosensor has been proposed based on the receptor protein adsorbed directly on a porous gold film. The film was produced electrochemically on a glassy carbon electrode in 0.08 M hydrogen tetrachloroaurate solution containing 0.004 M lead acetate with applied potential of −0.5 V (versus Ag/AgCl) for 50 s. The assay was carried out based on a sandwich procedure. The impedance signals amplified by precipitation of an insoluble product on the electrode showed good linearity with the content of IgG in the range of 0.011–11 ng mL −1 with a detection limit of 0.009 ng mL −1 . The gold film was characterized by scanning electron microscopy and the results showed porous gold nanoclusters were electrochemically deposited on the glassy carbon electrode.

[1]  G. Shen,et al.  Bacteria-modified Amperometric Immunosensor for a Brucella melitensis Antibody Assay , 2002, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[2]  B. Kamp,et al.  Development of an electrochemical immunosensor for direct detection of interferon-γ at the attomolar level , 2001 .

[3]  Zeev Rosenzweig,et al.  Development of an aggregation-based immunoassay for anti-protein A using gold nanoparticles. , 2002, Analytical chemistry.

[4]  Nicole Jaffrezic-Renault,et al.  Gold electrode functionalized by electropolymerization of a cyano N-substituted pyrrole: application to an impedimetric immunosensor , 2001 .

[5]  I. Willner,et al.  Liposomes labeled with biotin and horseradish peroxidase: a probe for the enhanced amplification of antigen--antibody or oligonucleotide--DNA sensing processes by the precipitation of an insoluble product on electrodes. , 2001, Analytical chemistry.

[6]  I. Willner,et al.  Electrochemical and quartz crystal microbalance detection of the cholera toxin employing horseradish peroxidase and GM1-functionalized liposomes. , 2001, Analytical chemistry.

[7]  Lin He,et al.  Colloidal Au-Enhanced Surface Plasmon Resonance for Ultrasensitive Detection of DNA Hybridization , 2000 .

[8]  I. Willner,et al.  Chronopotentiometry and Faradaic impedance spectroscopy as methods for signal transduction in immunosensors , 2001 .

[9]  R. Abuknesha,et al.  Biochemical aspects of biosensors. , 1994, Biosensors & bioelectronics.

[10]  M. Porter,et al.  Femtomolar detection of prostate-specific antigen: an immunoassay based on surface-enhanced Raman scattering and immunogold labels. , 2003, Analytical chemistry.

[11]  Jean-Michel Friedt,et al.  Realization and characterization of porous gold for increased protein coverage on acoustic sensors. , 2004, Analytical chemistry.

[12]  B. Limoges,et al.  An electrochemical metalloimmunoassay based on a colloidal gold label. , 2000, Analytical chemistry.

[13]  P. He,et al.  Colloid Au-enhanced DNA immobilization for the electrochemical detection of sequence-specific DNA , 2001 .

[14]  Yanbin Li,et al.  Interdigitated Array microelectrode-based electrochemical impedance immunosensor for detection of Escherichia coli O157:H7. , 2004, Analytical chemistry.

[15]  L. Authier,et al.  Gold nanoparticle-based quantitative electrochemical detection of amplified human cytomegalovirus DNA using disposable microband electrodes. , 2001, Analytical chemistry.

[16]  M. Porter,et al.  Immunoassay readout method using extrinsic Raman labels adsorbed on immunogold colloids. , 1999, Analytical chemistry.

[17]  M Aizawa,et al.  Assembling of engineered IgG-binding protein on gold surface for highly oriented antibody immobilization. , 2000, Journal of biotechnology.

[18]  Y. Chai,et al.  Study on the immobilization of anti-IgG on Au-colloid modified gold electrode via potentiometric immunosensor, cyclic voltammetry, and electrochemical impedance techniques. , 2005, Colloids and surfaces. B, Biointerfaces.

[19]  Kefeng Zeng,et al.  Magnetoelastic immunosensors: amplified mass immunosorbent assay for detection of Escherichia coli O157:H7. , 2003, Analytical chemistry.

[20]  C. Shannon,et al.  Heterogeneous immunosensing using antigen and antibody monolayers on gold surfaces with electrochemical and scanning probe detection. , 2000, Analytical chemistry.

[21]  T. Beebe,et al.  Specific Interactions between Biotin and Avidin Studied by Atomic Force Microscopy Using the Poisson Statistical Analysis Method , 1999 .

[22]  Y. Chai,et al.  Study on electrochemical behavior of a diphtheria immunosensor based on silica/silver/gold nanoparticles and polyvinyl butyral as matrices , 2005 .