Complete Nucleotide Sequence Analysis of Outer Membrane Efflux Protein Gene of 1347bp ORF from Riemerella anatipestifer

A 1347bp complete open reading frame of the Riemerella anatipestifer outer membrane efflux protein﹙GenBank No.CP003388﹚was analyzed by bioinformatics software. The gene encoded a protein of 448 amino acids, Molecular weight was 50811.1. The formula of the outer membrane efflux protein gene is C2265H3639N609O689S12. There was no transmembrane region and signal peptide. six N-glycosylation sites, one cAMP- and cGMP-dependent protein kinase phosphorylation site, eight Protein kinase C phosphorylation sites, ten Casein kinase II phosphorylation sites, four Tyrosine kinase phosphorylation sites, five N-myristoylation sites, one Cell attachment sequence. The sequence of Riemerella anatipestifer outer membrane efflux protein shared high homology with other members of flavobacterium. These results will lay the foundation for further study.

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