Enhanced performance of hexavalent chromium reducing cathodes in the presence of Shewanella oneidensis MR-1 and lactate.

Biocathodes for the reduction of the highly toxic hexavalent chromium (Cr(VI)) were investigated using Shewanella oneidensis MR-1 (MR-1) as a biocatalyst and performance was assessed in terms of current production and Cr(VI) reduction. Potentiostatically controlled experiments (-500 mV vs Ag/AgCl) showed that a mediatorless MR-1 biocathode started up under aerated conditions in the presence of lactate, received 5.5 and 1.7 times more electrons for Cr(VI) reduction over a 4 h operating period than controls without lactate and with lactate but without MR-1, respectively. Cr(VI) reduction was also enhanced, with a decrease in concentration over the 4 h operating period of 9 mg/L Cr(VI), compared to only 1 and 3 mg/L, respectively, in the controls. Riboflavin, an electron shuttle mediator naturally produced by MR-1, was also found to have a positive impact in potentiostatically controlled cathodes. Additionally, a microbial fuel cell (MFC) with MR-1 and lactate present in both anode and cathode produced a maximum current density of 32.5 mA/m(2) (1000 Ω external load) after receiving a 10 mg/L Cr(VI) addition in the cathode, and cathodic efficiency increased steadily over an 8 day operation period with successive Cr(VI) additions. In conclusion, effective and continuous Cr(VI) reduction with associated current production were achieved when MR-1 and lactate were both present in the biocathodes.

[1]  Yuehe Lin,et al.  Extracellular Reduction of Hexavalent Chromium by Cytochromes MtrC and OmcA of Shewanella oneidensis MR-1 , 2011, Applied and Environmental Microbiology.

[2]  A. Kaksonen,et al.  Ano-cathodophilic biofilm catalyzes both anodic carbon oxidation and cathodic denitrification. , 2012, Environmental science & technology.

[3]  David M Kramer,et al.  Formation of soluble organo-chromium(III) complexes after chromate reduction in the presence of cellular organics. , 2005, Environmental science & technology.

[4]  Liping Huang,et al.  Evaluation of carbon-based materials in tubular biocathode microbial fuel cells in terms of hexavalent chromium reduction and electricity generation , 2011 .

[5]  D. R. Bond,et al.  The Mtr Respiratory Pathway Is Essential for Reducing Flavins and Electrodes in Shewanella oneidensis , 2009, Journal of bacteriology.

[6]  K. Nealson,et al.  Current production by bacterial communities in microbial fuel cells enriched from wastewater sludge with different electron donors. , 2011, Environmental science & technology.

[7]  Largus T Angenent,et al.  Aerated Shewanella oneidensis in continuously fed bioelectrochemical systems for power and hydrogen production , 2010, Biotechnology and bioengineering.

[8]  Stefano Freguia,et al.  Microbial fuel cells: methodology and technology. , 2006, Environmental science & technology.

[9]  J. Gralnick,et al.  Ecology and biotechnology of the genus Shewanella. , 2007, Annual review of microbiology.

[10]  J. N. Petersen,et al.  Modeling chromate reduction in Shewanella oneidensis MR‐1: Development of a novel dual‐enzyme kinetic model , 2003, Biotechnology and bioengineering.

[11]  W. Verstraete,et al.  Microbial fuel cells: novel biotechnology for energy generation. , 2005, Trends in biotechnology.

[12]  Yahia Z. Hamada,et al.  Interaction of Malate and Lactate with Chromium(III) and Iron(III) in Aqueous Solutions , 2005 .

[13]  Guohua Chen,et al.  Effect of set potential on hexavalent chromium reduction and electricity generation from biocathode microbial fuel cells. , 2011, Environmental science & technology.

[14]  Zhiguo Yuan,et al.  Sequential anode-cathode configuration improves cathodic oxygen reduction and effluent quality of microbial fuel cells. , 2008, Water research.

[15]  R. Worden,et al.  Soluble electron shuttles can mediate energy taxis toward insoluble electron acceptors. , 2012, Environmental science & technology.

[16]  Justin C. Biffinger,et al.  Oxygen exposure promotes fuel diversity for Shewanella oneidensis microbial fuel cells. , 2008, Biosensors & bioelectronics.

[17]  T. D. Yuzvinsky,et al.  Electrical transport along bacterial nanowires from Shewanella oneidensis MR-1 , 2010, Proceedings of the National Academy of Sciences.

[18]  W. Verstraete,et al.  Continuous microbial fuel cells convert carbohydrates to electricity. , 2005, Water science and technology : a journal of the International Association on Water Pollution Research.

[19]  Gang Wang,et al.  Cathodic reduction of hexavalent chromium [Cr(VI)] coupled with electricity generation in microbial fuel cells , 2008, Biotechnology Letters.

[20]  Xingwang Zhang,et al.  Electricity production during the treatment of real electroplating wastewater containing Cr6+ using microbial fuel cell , 2008 .

[21]  D. Lovley,et al.  Electricity generation by direct oxidation of glucose in mediatorless microbial fuel cells , 2003, Nature Biotechnology.

[22]  Liping Huang,et al.  Enhancement of hexavalent chromium reduction and electricity production from a biocathode microbial fuel cell , 2010, Bioprocess and biosystems engineering.

[23]  S. Freguia,et al.  Cathodic oxygen reduction catalyzed by bacteria in microbial fuel cells , 2008, The ISME Journal.

[24]  K. Nealson,et al.  Evaluation of microbial fuel cell Shewanella biocathodes for treatment of chromate contamination , 2012 .

[25]  R. McCreery,et al.  Inhibition of Corrosion-Related Reduction Processes via Chromium Monolayer Formation , 2002 .

[26]  Kenji Kano,et al.  Electron transfer pathways in microbial oxygen biocathodes , 2010 .

[27]  Shi Liang,et al.  導電性ナノワイヤーをShewanella oneidensis菌MR‐1菌株その他の微生物が生成する , 2006 .

[28]  J. Lloyd,et al.  Secretion of Flavins by Shewanella Species and Their Role in Extracellular Electron Transfer , 2007, Applied and Environmental Microbiology.

[29]  K. Hashimoto,et al.  Flavins secreted by bacterial cells of Shewanella catalyze cathodic oxygen reduction. , 2012, ChemSusChem.

[30]  Sang-Eun Oh,et al.  Power generation using different cation, anion, and ultrafiltration membranes in microbial fuel cells. , 2007, Environmental science & technology.

[31]  S. Pavlostathis,et al.  Biological chromium(VI) reduction in the cathode of a microbial fuel cell. , 2009, Environmental science & technology.

[32]  Wesley C. Sanders,et al.  The utility of Shewanella japonica for microbial fuel cells. , 2011, Bioresource technology.

[33]  G. Ozolins,et al.  WHO guidelines for drinking-water quality. , 1984, WHO chronicle.

[34]  Tonni Agustiono Kurniawan,et al.  PHYSICO-CHEMICAL TREATMENT TECHNIQUES FOR WASTEWATER LADEN WITH HEAVY METALS , 2006 .

[35]  D. R. Bond,et al.  Shewanella secretes flavins that mediate extracellular electron transfer , 2008, Proceedings of the National Academy of Sciences.

[36]  R. McCreery,et al.  Raman Spectroscopy of Monolayers Formed from Chromate Corrosion Inhibitor on Copper Surfaces , 2003 .