VH gene family utilization is regulated by a locus outside of the VH region
暂无分享,去创建一个
We have used the RNA colony blot method to examine VH usage in colonies derived from primary splenic B cells. We found that there are strain- specific differences in the pattern of VH usage. Using parental F1, congenic, and recombinant inbred strains we demonstrate that the genetic element that causes the observed phenotype is: (a) stably expressed; (b) not due to maternal influence; (c) not due to dominate diffusible factors; (d) not linked to cloning efficiency; and (e) outside the Igh locus.
[1] L. Hood,et al. One heavy chain variable region gene segment subfamily in the BALB c mouse contains 500–1000 or more members , 1986, Cell.
[2] S. Tonegawa,et al. Somatic generation of antibody diversity. , 1976, Nature.