Pore size and properties of spherical Ca-alginate biocatalysts

SummaryA method for the production of spherical alginate gels from few μm up to several mm diameter is described. Alginate beads with diameters from 0.5 to 3 mm are used to immobilize microorganisms as biocatalysts. Micro size alginate gels are used as column packing material for inverse steric exclusion chromatography to determine their pore sizes from dextran standards of known molecular weight. The results indicate that alginate gels have pores which are large enough to release catalytic acting enzymes out of the matrix.

[1]  K. Vorlop,et al.  Immobilized Cells: Catalyst Preparation and Reaction Performance , 1983 .

[2]  S. Fukushima,et al.  Pilot Operation for Continuous Alcohol Fermentation of Molasses in an Immobilized Bioreactor , 1982 .

[3]  K. Vorlop,et al.  New Developments in the Field of Cell Immobilization — Formation of Biocatalysts by Ionotropic Gelation , 1983 .

[4]  H. Sahm,et al.  Immobilization of the methanogenic bacterium Methanosarcina barkeri , 1981 .

[5]  R. Messing Immobilized enzymes for industrial reactors , 1975 .

[6]  J. Klein,et al.  Immobilisation of microbial cells in polymeric matrices , 1975, European Journal of Applied Microbiology and Biotechnology.

[7]  R. Messing Simultaneously immobilized glucose oxidase and catalase in controlled-pore titania. , 1974, Biotechnology and bioengineering.

[8]  K. O. Pedersen Exclusion chromatography. , 1962, Archives of biochemistry and biophysics.

[9]  C. Bucke,et al.  The immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels , 1977, Biotechnology and bioengineering.

[10]  P. Cheetham Physical studies on the mechanical stability of columns of calcium alginate gel pellets containing entrapped microbial cells , 1979 .

[11]  J. Klein,et al.  New Developments in the Preparation and Characterization of Polymerbound Biocatalysts , 1982 .

[12]  K. Mosbach,et al.  Immobilized plant cells , 1982, Applied biochemistry and biotechnology.

[13]  John W. Dolan,et al.  Introduction to modern liquid chromatography , 1974 .