Industrial and biotechnological applications of laccases: a review.

Laccases have received much attention from researchers in last decades due to their ability to oxidise both phenolic and non-phenolic lignin related compounds as well as highly recalcitrant environmental pollutants, which makes them very useful for their application to several biotechnological processes. Such applications include the detoxification of industrial effluents, mostly from the paper and pulp, textile and petrochemical industries, use as a tool for medical diagnostics and as a bioremediation agent to clean up herbicides, pesticides and certain explosives in soil. Laccases are also used as cleaning agents for certain water purification systems, as catalysts for the manufacture of anti-cancer drugs and even as ingredients in cosmetics. In addition, their capacity to remove xenobiotic substances and produce polymeric products makes them a useful tool for bioremediation purposes. This paper reviews the applications of laccases within different industrial fields as well as their potential extension to the nanobiotechnology area.

[1]  S. R. Couto,et al.  Utilisation of grape seeds for laccase production in solid-state fermentors , 2006 .

[2]  M. A. Sanromán,et al.  Stainless steel sponge: a novel carrier for the immobilisation of the white-rot fungus Trametes hirsuta for decolourization of textile dyes. , 2004, Bioresource technology.

[3]  L. Gorton,et al.  CHARACTERIZATION OF GRAPHITE ELECTRODES MODIFIED WITH LACCASE FROM TRAMETES VERSICOLOR AND THEIR USE FOR BIOELECTROCHEMICAL MONITORING OF PHENOLIC COMPOUNDS IN FLOW INJECTION ANALYSIS , 2003 .

[4]  I. Herpoël,et al.  Pycnoporus cinnabarinus laccases: an interesting tool for food or non-food applications. , 2003, Communications in agricultural and applied biological sciences.

[5]  V. Micard,et al.  Oxidative gelation of sugar-beet pectins : use of laccases and hydration properties of the cross-linked pectins , 1999 .

[6]  V. I. Berezin,et al.  Peroxidase and laccase as catalysts for removal of the phenylhydrazide protecting group under mild conditions , 1993, Biotechnology and bioengineering.

[7]  Giovanni Sannia,et al.  Remazol Brilliant Blue R decolourisation by the fungus Pleurotus ostreatus and its oxidative enzymatic system , 2005 .

[8]  A. Mucci,et al.  Biotransformation of resveratrol: synthesis of trans-dehydrodimers catalyzed by laccases from Myceliophtora thermophyla and from Trametes pubescens , 2004 .

[9]  Ibrahim M. Banat,et al.  Microbial decolorization of textile-dye-containing effluents A review , 1996 .

[10]  Shiro Kobayashi,et al.  Enzyme-catalyzed polymerization to functional polymers , 2002 .

[11]  C. Johannes,et al.  Natural Mediators in the Oxidation of Polycyclic Aromatic Hydrocarbons by Laccase Mediator Systems , 2000, Applied and Environmental Microbiology.

[12]  Federico Federici,et al.  Panus tigrinus efficiently removes phenols, color and organic load from olive-mill wastewater. , 2004, Research in microbiology.

[13]  D. Leech,et al.  Reagentless mediated laccase electrode for the detection of enzyme modulators. , 1997, Analytical chemistry.

[14]  H. Möhwald,et al.  Polyelectrolyte multilayer capsule permeability control , 2002 .

[15]  C. Galli,et al.  Free radical versus electron-transfer routes of oxidation of hydrocarbons by laccase/mediator systems. Catalytic or stoichiometric procedures , 2003 .

[16]  A. Kandelbauer,et al.  Indigo degradation with purified laccases from Trametes hirsuta and Sclerotium rolfsii. , 2001, Journal of biotechnology.

[17]  Azmi Telefoncu,et al.  Laccase : Production by Trametes versicolor and application to denim washing , 2005 .

[18]  V. Chaplain,et al.  Interference of Soil Contaminants with Laccase Activity During the Transformation of Complex Mixtures of Polycyclic Aromatic Hydrocarbons in Liquid Media , 2001 .

[19]  R. Casadio,et al.  Isothermal and non-isothermal bioreactors in the detoxification of waste waters polluted by aromatic compounds by means of immobilised laccase from Rhus vernicifera , 2004 .

[20]  G. Soares,et al.  Use of laccase together with redox mediators to decolourize Remazol Brilliant Blue R. , 2001, Journal of biotechnology.

[21]  S. Mansfield,et al.  Degradation of trilinolein by laccase enzymes. , 2002, Archives of biochemistry and biophysics.

[22]  C. Felby,et al.  Enhanced Auto Adhesion of Wood Fibers Using Phenol Oxidases , 1997 .

[23]  T. Eggen Application of fungal substrate from commercial mushroom production — Pleuorotus ostreatus — for bioremediation of creosote contaminated soil , 1999 .

[24]  G. Mckay,et al.  The Removal of Acid Dye from Effluent Using Natural Adsorbents. I. Peat. , 1976 .

[25]  N. Durán,et al.  Decolorization of reactive dyes by immobilized laccase , 2003 .

[26]  A. Messina,et al.  Oxidation of ferulic acid by laccase: identification of the products and inhibitory effects of some dipeptides. , 2001, Talanta.

[27]  F. Scheller,et al.  New enzyme sensors for morphine and codeine based on morphine dehydrogenase and laccase , 1999 .

[28]  E. A. Erkurt,et al.  A Drimaren Blue X3LR dye decolorizing enzyme from Funalia trogii: one step isolation and identification , 2005 .

[29]  K. Itoh,et al.  Phenolic acids affect transformations of chlorophenols by a Coriolus versicolor laccase , 2000 .

[30]  G. Soares,et al.  Decolorization of an anthraquinone-type dye using a laccase formulation. , 2001, Bioresource technology.

[31]  G. Aggelis,et al.  Phenolic removal in olive oil mill wastewater by strains of Pleurotus spp. in respect to their phenol oxidase (laccase) activity. , 2002, Bioresource technology.

[32]  D. Leech,et al.  Optimisation of a reagentless laccase electrode for the detection of the inhibitor azide. , 1998, The Analyst.

[33]  F. Schauer,et al.  Synthesis of substituted imidazoles and dimerization products using cells and laccase from Trametes versicolor , 2001 .

[34]  M. Pizzigallo,et al.  Polyphenoloxidases immobilized in organic gels: Properties and applications in the detoxification of aromatic compounds , 1995, Biotechnology and bioengineering.

[35]  Yumin Du,et al.  Flow-injection stopped-flow spectrofluorimetric kinetic determination of total ascorbic acid based on an enzyme-linked coupled reaction , 1995 .

[36]  W. Steiner,et al.  Production of laccase by a newly isolated strain of Trametes modesta. , 2002, Bioresource technology.

[37]  María Jesús Martínez,et al.  Efficient bleaching of non-wood high-quality paper pulp using laccase-mediator system , 2004 .

[38]  Kuznetsov,et al.  On applicability of laccase as label in the mediated and mediatorless electroimmunoassay: effect of distance on the direct electron transfer between laccase and electrode. , 2001, Biosensors & bioelectronics.

[39]  Feng-Chin Wu,et al.  Use of chitin and chitosan in lobster shell wastes for color removal from aqueous solutions , 1996 .

[40]  D. Barceló,et al.  Determination of sulfonated azo dyes in groundwater and industrial effluents by automated solid‐phase extraction followed by capillary electrophoresis/mass spectrometry , 1998 .

[41]  R. Bourbonnais,et al.  Electrochemical analysis of the interactions of laccase mediators with lignin model compounds. , 1998, Biochimica et biophysica acta.

[42]  H Hörtnagl,et al.  Catecholamine detection using enzymatic amplification. , 1997, Biosensors & bioelectronics.

[43]  Emilio Rosales,et al.  Exploitation of a waste from the brewing industry for laccase production by two Trametes species , 2004 .

[44]  M. Kleen,et al.  Chemistry of mediated-laccase delignification analyzed by pyrolysis-GC/MS , 2003 .

[45]  J. Buchert,et al.  Effects of laccase, xylanase and their combination on the rheological properties of wheat doughs , 2006 .

[46]  W. Leukes,et al.  Ultrafiltration of petrochemical industrial wastewater using immobilised manganese peroxidase and laccase: application in the defouling of polysulphone membranes☆ , 2002 .

[47]  B. Harte,et al.  Development of a gas-phase oxygen biosensor using a blue copper-containing oxidase. , 1996, Enzyme and microbial technology.

[48]  A. Tanyolaç,et al.  Reaction conditions for laccase catalyzed polymerization of catechol. , 2003, Bioresource technology.

[49]  P. P. Gallego,et al.  Grape seeds: the best lignocellulosic waste to produce laccase by solid state cultures of Trametes hirsuta , 2003, Biotechnology Letters.

[50]  K. Patel,et al.  Decolorization of synthetic dyes by Irpex lacteus in liquid cultures and packed-bed bioreactor , 2003 .

[51]  C. Johannes,et al.  Oxidation of Polycyclic Aromatic Hydrocarbons (PAH) by Laccase of Trametes Versicolor , 1998 .

[52]  E. Veignie,et al.  Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by Cladosporium sphaerospermum isolated from an aged PAH contaminated soil. , 2004, FEMS microbiology ecology.

[53]  Mai,et al.  Chemo-enzymatic synthesis and characterization of graft copolymers from lignin and acrylic compounds. , 2000, Enzyme and microbial technology.

[54]  James E. Sealey,et al.  Investigations into Laccase-Mediator Delignification of Kraft Pulps , 1999 .

[55]  A. Gaspar,et al.  A novel approach for the oxidative catalysis employing polyoxometalate–laccase system: application to the oxygen bleaching of kraft pulp , 2004 .

[56]  N. Durán,et al.  Potential applications of laccase in the food industry , 2002 .

[57]  Y. Takashima,et al.  Degradation of bisphenol A by purified laccase from Trametes villosa. , 2001, Biochemical and biophysical research communications.

[58]  S. Rodríguez Couto,et al.  Influence of redox mediators and metal ions on synthetic acid dye decolourization by crude laccase from Trametes hirsuta. , 2005, Chemosphere.

[59]  Ángel T. Martínez,et al.  Role of Pycnoporus coccineus laccase in the degradation of aromatic compounds in olive oil mill wastewater , 2005 .

[60]  S. Ekunwe,et al.  Oxidation of anthracene and benzo[a]pyrene by immobilized laccase from Trametes versicolor , 2004 .

[61]  Katsuya Kato,et al.  Detoxification of bisphenol A and nonylphenol by purified extracellular laccase from a fungus isolated from soil. , 2004, Journal of bioscience and bioengineering.

[62]  A. Krastanov,et al.  Biodegradation of phenols by laccase immobilised in a membrane reactor , 2000 .

[63]  A. Yaropolov,et al.  Laccase: properties, catalytic mechanism, and applicability , 1994 .

[64]  G. Soares,et al.  Studies on the biotransformation of novel disazo dyes by laccase , 2002 .

[65]  G. Guebitz,et al.  Coupling of 2,4,6-trinitrotoluene (TNT) metabolites onto humic monomers by a new laccase from Trametes modesta. , 2006, Chemosphere.

[66]  T. Fujii,et al.  Treatment of model soils contaminated with phenolic endocrine-disrupting chemicals with laccase from Trametes sp. in a rotating reactor. , 2001, Journal of bioscience and bioengineering.

[67]  A Heller,et al.  A miniature biofuel cell. , 2001, Journal of the American Chemical Society.

[68]  Helmuth Möhwald,et al.  Novel Hollow Polymer Shells by Colloid-Templated Assembly of Polyelectrolytes. , 1998, Angewandte Chemie.

[69]  T. E. Abraham,et al.  Biosensor for the determination of phenols based on cross-linked enzyme crystals (CLEC) of laccase. , 2005, Biosensors & bioelectronics.

[70]  M. Rebelo,et al.  An amperometric biosensor for polyphenolic compounds in red wine. , 2004, Biosensors & bioelectronics.

[71]  M. Lund,et al.  Enzymatic modification of kraft lignin through oxidative coupling with water-soluble phenols , 2001, Applied Microbiology and Biotechnology.

[72]  George L. Baughman,et al.  Fate of dyes in aquatic systems. I: Solubility and partitioning of some hydrophobic dyes and related compounds , 1988 .

[73]  S. R. Couto,et al.  Effect of two wastes from groundnut processing on laccase production and dye decolourisation ability , 2006 .

[74]  M. Goto,et al.  Oxidation of bisphenol A catalyzed by laccase hosted in reversed micelles in organic media , 2002 .

[75]  Michael C. Flickinger,et al.  Encyclopedia of bioprocess technology : fermentation, biocatalysis, and bioseparation , 1999 .

[76]  A. Tanyolaç,et al.  Reaction kinetics for laccase-catalyzed polymerization of 1-naphthol. , 2001, Bioresource technology.

[77]  Gautam Gupta,et al.  Laccase Biosensor on Monolayer-Modified Gold Electrode , 2003 .

[78]  Shiro Kobayashi,et al.  Enzymatic synthesis and antioxidant properties of poly(rutin). , 2003, Biomacromolecules.

[79]  M. K. Vijayeendra,et al.  Discharge behaviour of single and combination colours used in silk industry , 2001 .

[80]  M. A. Sanromán,et al.  Chestnut shell and barley bran as potential substrates for laccase production by Coriolopsis rigida under solid-state conditions , 2005 .

[81]  L. Gorton,et al.  Development of enzyme-based amperometric sensors for the determination of phenolic compounds , 1995 .

[82]  A. Kirkman,et al.  Biobleaching of pulp with dioxygen in laccase-mediator system—effect of variables on the reaction kinetics , 2001 .

[83]  A. Ragauskas,et al.  Laccase‐Mediator Biobleaching Applied to a Direct Yellow Dyed Paper , 2004 .

[84]  G. Gübitz,et al.  Application of power ultrasound for azo dye degradation. , 2004, Ultrasonics sonochemistry.

[85]  T. Tzanov,et al.  Immobilized laccase for decolourization of Reactive Black 5 dyeing effluent , 2003, Biotechnology Letters.

[86]  L. Gorton,et al.  Amperometric detection of mono- and diphenols at Cerrena unicolor laccase-modified graphite electrode: correlation between sensitivity and substrate structure. , 2005, Talanta.

[87]  L. Viikari,et al.  Enzymatic oxidation of alkenes , 2000 .

[88]  U. Wollenberger,et al.  Electroanalytical determination of peroxidases and laccases on carbon paste electrodes , 1997 .

[89]  Scott Calabrese Barton,et al.  Electroreduction of O(2) to water at 0.6 V (SHE) at pH 7 on the "wired" Pleurotus ostreatus laccase cathode. , 2002, Biosensors & bioelectronics.

[90]  F. Caruso,et al.  Enzyme encapsulation in layer-by-layer engineered polymer multilayer capsules. , 2000 .

[91]  Baker,et al.  Transformation and degradation of the disazo dye Chicago Sky Blue by a purified laccase from Pycnoporus cinnabarinus. , 2000, Enzyme and microbial technology.

[92]  S. Allen,et al.  Electrooxidation of dyestuffs in waste waters , 1995 .

[93]  C. Johannes,et al.  Oxidation of acenaphthene and acenaphthylene by laccase of Trametes versicolor in a laccase-mediator system , 1998 .

[94]  A. D’Annibale,et al.  Oxirane-immobilized Lentinula edodes laccase: stability and phenolics removal efficiency in olive mill wastewater. , 2000, Journal of biotechnology.

[95]  E. Hammer,et al.  Synthesis of 3-(3,4-dihydroxyphenyl)-propionic acid derivatives by N-coupling of amines using laccase , 2002 .

[96]  M. Arenas,et al.  Kraft Pulp Biobleaching and Mediated Oxidation of a Nonphenolic Substrate by Laccase from Streptomyces cyaneus CECT 3335 , 2003, Applied and Environmental Microbiology.

[97]  O. Fatibello‐Filho,et al.  Synergic effect studies of the bi-enzymatic system laccase-peroxidase in a voltammetric biosensor for catecholamines. , 2003, Talanta.

[98]  K. Messner,et al.  Oxidation of phenanthrene by a fungal laccase in the presence of 1-hydroxybenzotriazole and unsaturated lipids. , 1998, Biochemical and biophysical research communications.

[99]  L. Gorton,et al.  Use of laccase-modified electrode for amperometric detection of plant flavonoids , 2004 .

[100]  Y. Rhee,et al.  Oxidation of polycyclic aromatic hydrocarbons by laccase of Coriolus hirsutus , 2002, Biotechnology Letters.

[101]  Gero Decher,et al.  Fuzzy Nanoassemblies: Toward Layered Polymeric Multicomposites , 1997 .

[102]  M. Pickard,et al.  Hydroxybenzotriazole increases the range of textile dyes decolorized by immobilized laccase , 1999, Biotechnology Letters.

[103]  E. Solomon,et al.  Multicopper Oxidases and Oxygenases. , 1996, Chemical reviews.

[104]  S. R. Couto,et al.  Dye decolorization by Trametes hirsuta immobilized into alginate beads , 2005 .

[105]  I. Reid,et al.  Kraft pulp bleaching and delignification by Trametes versicolor , 1997 .

[106]  G. Tayhas R. Palmore,et al.  Electro-enzymatic reduction of dioxygen to water in the cathode compartment of a biofuel cell , 1999 .

[107]  R. Baets,et al.  Controlled patterning of biomolecules on solid surfaces , 2003 .

[108]  N. Durán,et al.  Effects of fungal laccase immobilization procedures for the development of a biosensor for phenol compounds. , 2001, Talanta.

[109]  L. Golovleva,et al.  Biotransformation of soil humic acids by blue laccase of Panus tigrinus 8/18: an in vitro study , 2004 .

[110]  Cliona O’Neill,et al.  Colour in textile effluents – sources, measurement, discharge consents and simulation: a review , 1999 .

[111]  C. Jolivalt Immobilization of laccase from Trametes versicolor on a modified PVDF microfiltration membrane: characterization of the grafted support and application in removing a phenylurea pesticide in wastewater , 2000 .

[112]  Ana S. Viana,et al.  N-Hydroxysuccinimide-terminated self-assembled monolayers on gold for biomolecules immobilisation , 2005 .

[113]  H. Call,et al.  History, overview and applications of mediated lignolytic systems, especially laccase-mediator-systems (Lignozym-process) , 1997 .

[114]  A. Johnson,et al.  Performance parameters of oxygen delignification , 1997 .

[115]  Ashutosh Kumar Singh,et al.  Microorganisms and enzymes involved in the degradation of plant fiber cell walls. , 1997, Advances in biochemical engineering/biotechnology.

[116]  S. Papanikolaou,et al.  Phenolic removal in a model olive oil mill wastewater using Pleurotus ostreatus in bioreactor cultures and biological evaluation of the process. , 2003, Water research.

[117]  Feng Xu,et al.  Comparison of Fungal Laccases and Redox Mediators in Oxidation of a Nonphenolic Lignin Model Compound , 1999, Applied and Environmental Microbiology.

[118]  L. Setti,et al.  Laccase catalyzed-oxidative coupling of 3-methyl 2-benzothiazolinone hydrazone and methoxyphenols , 1999 .

[119]  J. Bollag,et al.  Transformation of the fungicide cyprodinil by a laccase of Trametes villosa in the presence of phenolic mediators and humic acid. , 2002, Water research.

[120]  L. Muniglia,et al.  Phenolic colorants obtained by enzymatic synthesis using a fungal laccase in a hydro-organic biphasic system , 2005 .

[121]  Laccase catalyzes formation of an indamine dye between 3-methyl-2-benzothiazolinone hydrazone and 3-dimethylaminobenzoic acid , 1996 .

[122]  G. Whitesides,et al.  Formation of polymer microstructures by selective deposition of polyion multilayers using patterned self-assembled monolayers as a template , 1995 .

[123]  M. A. Sanromán,et al.  Screening of supports and inducers for laccase production by Trametes versicolor in semi-solid-state conditions , 2002 .

[124]  D. Leech,et al.  Amperometric Detection of Catecholamine Neurotransmitters Using Electrocatalytic Substrate Recycling at a Laccase Electrode , 2005 .

[125]  J. Kulys,et al.  Laccase-catalyzed oxidation of naphthol in the presence of soluble polymers , 2003 .

[126]  K. Murugesan Bioremediation of paper and pulp mill effluents. , 2003, Indian journal of experimental biology.

[127]  S. Pointing,et al.  Feasibility of bioremediation by white-rot fungi , 2001, Applied Microbiology and Biotechnology.

[128]  M. Pickard,et al.  Polycyclic Aromatic Hydrocarbon Metabolism by White Rot Fungi and Oxidation by Coriolopsis gallica UAMH 8260 Laccase , 1999, Applied and Environmental Microbiology.

[129]  F. Vianello,et al.  A high sensitivity amperometric biosensor using a monomolecular layer of laccase as biorecognition element. , 2004, Biosensors & bioelectronics.

[130]  S. R. Couto,et al.  Coconut flesh: a novel raw material for laccase production by Trametes hirsuta under solid-state conditions.: Application to Lissamine Green B decolourization , 2005 .

[131]  Martin Fischlechner,et al.  Engineering virus functionalities on colloidal polyelectrolyte lipid composites. , 2005, Angewandte Chemie.

[132]  Juozas Kulys,et al.  Amperometric biosensors based on recombinant laccases for phenols determination. , 2003, Biosensors & bioelectronics.

[133]  S. Ledakowicz,et al.  Decolorization of aqueous solution of dyes by the laccase complex from Cerrena unicolor , 2003 .

[134]  J. Adler-Nissen,et al.  ENZYMATIC GELATION OF SUGAR BEET PECTIN IN FOOD PRODUCTS , 2000 .

[135]  Ji-ti Zhou,et al.  Enhancement of laccase production by Pleurotus ostreatus and its use for the decolorization of anthraquinone dye , 2004 .

[136]  M. Suzuki,et al.  Polymerization of bisphenol A by purified laccase from Trametes villosa. , 2001, Biochemical and biophysical research communications.

[137]  M. Pickard,et al.  Enhanced activity by poly(ethylene glycol) modification of Coriolopsis gallica laccase , 2002, Journal of Industrial Microbiology and Biotechnology.

[138]  A. Ragauskas,et al.  Evaluating laccase-facilitated coupling of phenolic acids to high-yield kraft pulps , 2001 .

[139]  D. Pum,et al.  Recrystallization of bacterial S-layers on flat polyelectrolyte surfaces and hollow polyelectrolyte capsules. , 2005, Small.

[140]  C. Johannes,et al.  Radical mediated indirect oxidation of a PEG-coupled polycyclic aromatic hydrocarbon (PAH) model compound by fungal laccase. , 2000, Biochimica et biophysica acta.

[141]  Qing X. Li,et al.  Fungal laccase-catalyzed degradation of hydroxy polychlorinated biphenyls. , 2004, Chemosphere.

[142]  M. Fabbrini,et al.  An oxidation of alcohols by oxygen with the enzyme laccase and mediation by TEMPO , 2001 .

[143]  E. Fritz-Langhals,et al.  SYNTHESIS OF AROMATIC ALDEHYDES BY LACCASE-MEDIATOR ASSISTED OXIDATION , 1998 .

[144]  C. Crestini,et al.  The early oxidative biodegradation steps of residual kraft lignin models with laccase. , 1998, Bioorganic & medicinal chemistry.

[145]  T. Tzanov,et al.  Decolorization and Detoxification of Textile Dyes with a Laccase from Trametes hirsuta , 2000, Applied and Environmental Microbiology.

[146]  F. Schinner,et al.  Characterization and immobilization of the laccase from Pleurotus ostreatus and its use for the continuous elimination of phenolic pollutants. , 2000, Enzyme and microbial technology.

[147]  R. Pilloton,et al.  Thick film sensors based on laccases from different sources immobilized in polyaniline matrix , 2004 .

[148]  M. Moeder,et al.  Degradation of hydroxylated compounds using laccase and horseradish peroxidase immobilized on microporous polypropylene hollow fiber membranes , 2004 .

[149]  L. Levin,et al.  Ligninolytic enzyme production and dye decolorization by Trametes trogii: application of the Plackett–Burman experimental design to evaluate nutritional requirements , 2005 .

[150]  A. Hüttermann,et al.  Modification of lignin for the production of new compounded materials , 2001, Applied Microbiology and Biotechnology.

[151]  S. R. Couto,et al.  Production of Laccase by Trametes hirsuta Grown in an Immersion Bioreactor and its Application in the Docolorization of Dyes from a Leather Factory , 2004 .

[152]  N. Durán,et al.  Potential applications of oxidative enzymes and phenoloxidase-like compounds in wastewater and soil treatment: a review , 2000 .

[153]  S. R. Couto,et al.  Improving laccase production by employing different lignocellulosic wastes in submerged cultures of Trametes versicolor. , 2002, Bioresource technology.

[154]  G. Whitesides,et al.  Effect of Surface Wettability on the Adsorption of Proteins and Detergents , 1998 .

[155]  M. Sarrà,et al.  Mechanism of textile metal dye biotransformation by Trametes versicolor. , 2004, Water research.

[156]  T. Fujii,et al.  Treatment of nonylphenol with laccase in a rotating reactor. , 2003, Journal of bioscience and bioengineering.

[157]  A. Messerschmidt Multi-Copper Oxidases , 1997 .

[158]  J. Copa-Patiño,et al.  Studies of the production and characterization of laccase activity in the basidiomycete Coriolopsis gallica, an efficient decolorizer of alkaline effluents , 1998, Archives of Microbiology.

[159]  Feng Xu,et al.  Laccases: A Useful Group of Oxidoreductive Enzymes , 1999 .

[160]  D. Evtuguin,et al.  Degradation of biphenyl lignin model compounds by laccase of Trametes versicolor in the presence of 1-hydroxybenzotriazole and heteropolyanion [SiW11VO40]5- , 2003 .

[161]  R. Bourbonnais,et al.  Oxidation of non‐phenolic substrates , 1990, FEBS letters.

[162]  A. D’Annibale,et al.  Characterization of immobilized laccase from Lentinula edodes and its use in olive-mill wastewater treatment , 1999 .

[163]  R. Bourbonnais,et al.  Reactivities of various mediators and laccases with kraft pulp and lignin model compounds , 1997, Applied and environmental microbiology.

[164]  Blue Copper Oxidases , 1993 .

[165]  R. Casa,et al.  Reduction of the phenolic components in olive-mill wastewater by an enzymatic treatment and its impact on durum wheat (Triticum durum Desf.) germinability. , 2003, Chemosphere.

[166]  T. Reinikainen,et al.  Rheological properties of laccase-induced sugar beet pectin gels , 2003 .

[167]  Milan Mrksich,et al.  Micropatterned Surfaces for Control of Cell Shape, Position, and Function , 1998, Biotechnology progress.

[168]  H. König,et al.  Redox-mediated decolorization of synthetic dyes by fungal laccases , 2002, Applied Microbiology and Biotechnology.

[169]  S. Shleev,et al.  Laccase-catalyzed synthesis of conducting polyaniline , 2003 .

[170]  M. Rebelo,et al.  A New Laccase Biosensor For Polyphenols Determination , 2003 .

[171]  Lauro T. Kubota,et al.  Development of a laccase-based flow injection electrochemical biosensor for the determination of phenolic compounds and its application for monitoring remediation of Kraft E1 paper mill effluent , 2002 .

[172]  P. Collins,et al.  Oxidation of Anthracene and Benzo[a]pyrene by Laccases from Trametes versicolor , 1996, Applied and environmental microbiology.