Simultaneously Extraction of Caffeine and Chlorogenic Acid from Coffea canephora Bean using Natural Deep Eutectic Solvent-Based Ultrasonic Assisted Extraction

Background: NADES is an alternative solvent in the extraction of metabolites from plants which has many environmental benefits, such as low toxicity, biodegradability, can dissolve polar and non-polar compounds, low costs and simple preparation. Objective: This study aims to determine the effect of natural deep eutectic solvent-based ultrasonic-assisted extraction (NADES-UAE) on enrichment of caffeine and chlorogenic acid in extract from green coffee beans (Coffea canephora). Methods: The powders were extracted using NADES-UAE method in several types of extraction condition, including the composition of NADES, water addition in NADES and extraction time. Caffeine and chlorogenic acid content were analyzed using HPLC, reverse phase system and C18 ODS-3 column. Results: The highest of caffeine and chlorogenic acid content was respectively, 7.89 mg/g and 28.62 mg/g (composition of NADES betaine: sorbitol [1:1.2] ratio and NADES-water addition [1:2] ratio for 30 min). Conclusion: This research showed that the composition of NADES, extraction time and water addition are important parameter in extracting caffeine and chlorogenic acid content in green coffee beans.

[1]  Arifur Rahman,et al.  Application of Natural Deep Eutectic Solvent-Based Ultrasonic Assisted Extraction of Total Polyphenolic and Caffeine Content from Coffe Beans (Coffea Beans L.) For Instant Food Products , 2018, Journal of Applied Pharmaceutical Science.

[2]  Liang Wenhua,et al.  Chlorogenic acid (CGA): A pharmacological review and call for further research. , 2018, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[3]  M. Leone,et al.  Simultaneous Determination of Caffeine and Chlorogenic Acids in Green Coffee by UV/Vis Spectroscopy , 2017 .

[4]  A. A. Abd El-Aty,et al.  Determination of chlorogenic acids and caffeine in homemade brewed coffee prepared under various conditions. , 2017, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[5]  J. Rodricks,et al.  Caffeine and cardiovascular health , 2017, Regulatory toxicology and pharmacology : RTP.

[6]  R. Reis,et al.  Natural deep eutectic solvents from choline chloride and betaine – Physicochemical properties , 2017 .

[7]  Erwann Durand,et al.  Application of Deep Eutectic Solvents (DES) for Phenolic Compounds Extraction: Overview, Challenges, and Opportunities. , 2017, Journal of agricultural and food chemistry.

[8]  K. Mulia,et al.  Extraction of vitexin from binahong (Anredera cordifolia (Ten.) Steenis) leaves using betaine - 1,4 butanediol natural deep eutectic solvent (NADES) , 2017 .

[9]  D. Kitts,et al.  Performance review of a fast HPLC-UV method for the quantification of chlorogenic acids in green coffee bean extracts. , 2016, Talanta.

[10]  Ping Li,et al.  Comprehensive Evaluation of Deep Eutectic Solvents in Extraction of Bioactive Natural Products , 2016 .

[11]  María de los Ángeles Fernández,et al.  Natural designer solvents for greening analytical chemistry , 2016 .

[12]  Y. Choi,et al.  Tailoring properties of natural deep eutectic solvents with water to facilitate their applications. , 2015, Food chemistry.

[13]  Rui L. Reis,et al.  Natural Deep Eutectic Solvents – Solvents for the 21st Century , 2014 .

[14]  Geert-Jan Witkamp,et al.  Natural deep eutectic solvents as new potential media for green technology. , 2013, Analytica chimica acta.

[15]  T. Shibamoto,et al.  Role of roasting conditions in the level of chlorogenic acid content in coffee beans: correlation with coffee acidity. , 2009, Journal of agricultural and food chemistry.

[16]  D. Perrone,et al.  Comprehensive analysis of major and minor chlorogenic acids and lactones in economically relevant Brazilian coffee cultivars , 2008 .

[17]  Takayuki Shibamoto,et al.  Chlorogenic acid and caffeine contents in various commercial brewed coffees , 2008 .

[18]  A. Farah,et al.  Effect of roasting on the formation of chlorogenic acid lactones in coffee. , 2005, Journal of agricultural and food chemistry.