Bioelectrocatalytic application of titania nanotube array for molecule detection.

A bioelectrocatalysis system based on titania nanotube electrode has been developed for the quantitative detection application. Highly ordered titania nanotube array with inner diameter of 60 nm and total length of 540 nm was formed by anodizing titanium foils. The functionalization modification was achieved by embedding glucose oxidases inside tubule channels and electropolymerizing pyrrole for interfacial immobilization. Morphology and microstructure characterization, electrochemical properties and bioelectrocatalytic reactivities of this composite were fully investigated. The direct detection of hydrogen peroxide by electrocatalytic reduction reaction was fulfilled on pure titania nanotube array with a detection limit up to 2.0 x 10(-4)mM. A biosensor based on the glucose oxidase-titania/titanium electrode was constructed for amperometric detection and quantitative determination of glucose in a phosphate buffer solution (pH 6.8) under a potentiostatic condition (-0.4V versus SCE). The resulting glucose biosensor showed an excellent performance with a response time below 5.6s and a detection limit of 2.0 x 10(-3)mM. The corresponding detection sensitivity was 45.5 microA mM(-1)cm(-2). A good operational reliability was also achieved with relative standard deviations below 3.0%. This novel biosensor exhibited quite high response sensitivity and low detection limit for potential applications.

[1]  N. Jaffrezic‐Renault,et al.  Development of enzyme biosensor based on pH-sensitive field-effect transistors for detection of phenolic compounds. , 2002, Bioelectrochemistry.

[2]  Michael Grätzel,et al.  A glucose biosensor based on enzyme entrapment within polypyrrole films electrodeposited on mesoporous titanium dioxide , 1999 .

[3]  Si-hoon Lee,et al.  Fabrication of TiO2 Tubules by Template Synthesis and Hydrolysis with Water Vapor , 2004 .

[4]  M. K. Sezgintürk,et al.  A biosensor based on urate oxidase-peroxidase coupled enzyme system for uric acid determination in urine. , 2003, Talanta.

[5]  Zhengdong Sun,et al.  Immobilization of uricase on ZnO nanorods for a reagentless uric acid biosensor , 2004 .

[6]  P. Bianco,et al.  Application of the electrochemical concepts and techniques to amperometric biosensor devices , 2006 .

[7]  G. Luo,et al.  Amperometric Detection of Glucose with Glucose Oxidase Absorbed on Porous Nanocrystalline TiO2 Film , 2001 .

[8]  Huifang Xu,et al.  Large oriented arrays and continuous films of TiO(2)-based nanotubes. , 2003, Journal of the American Chemical Society.

[9]  Meital Reches,et al.  Peptide nanotube-modified electrodes for enzyme-biosensor applications. , 2005, Analytical chemistry.

[10]  B. Kasemo,et al.  Vesicle adsorption on SiO2 and TiO2: Dependence on vesicle size , 2002 .

[11]  J. Hubbell,et al.  Poly(l-lysine)-g-Poly(ethylene glycol) Layers on Metal Oxide Surfaces: Attachment Mechanism and Effects of Polymer Architecture on Resistance to Protein Adsorption† , 2000 .

[12]  Song Zhang,et al.  Ordered Mesoporous Niobium Oxide Film: A Novel Matrix for Assembling Functional Proteins for Bioelectrochemical Applications , 2003 .

[13]  M. Abidian,et al.  Conducting‐Polymer Nanotubes for Controlled Drug Release , 2006, Advanced materials.

[14]  Sungho Jin,et al.  Growth of nano-scale hydroxyapatite using chemically treated titanium oxide nanotubes. , 2005, Biomaterials.

[15]  Guo-Li Shen,et al.  Amperometric glucose biosensor based on a surface treated nanoporous ZrO2/Chitosan composite film as immobilization matrix , 2004 .

[16]  James R. Durrant,et al.  Immobilisation and bioelectrochemistry of proteins on nanoporous TiO2 and ZnO films , 2001 .

[17]  D. Schomburg,et al.  The 3D structure of glucose oxidase from Aspergillus niger. Implications for the use of GOD as a biosensor enzyme. , 1993, Biosensors & bioelectronics.

[18]  E Wilkins,et al.  Glucose monitoring: state of the art and future possibilities. , 1996, Medical engineering & physics.

[19]  William F. DeGrado.  Computational biology: Biosensor design , 2003, Nature.