Diversity amongst the microglia in growing and regenerating fish CNS: Immunohistochemical characterization using FL. 1, an anti‐macrophage monoclonal antibody

We have immunohistochemically characterized the forms and distribution of microglia—the macrophages of the CNS—in fish, using a new monoclonal antibody (mAb), FL.1. This mAb specifically reacts with resident macrophages throughout the body in Oreochromine fish, including Kupffer cells, gut‐associated myeloid cells, and peritoneal macrophages, as well as with microglia, but circulating monocytes are not labelled with FL.1. The FL.1‐epitope, which is lost following treatment with reducing agents, has an extracellular location and is associated with three integral membrane glycoprotein variants.

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