Buffy‐Coat‐Derived Platelet Concentrates Prepared from Half‐Strength Citrate CPD and CPD Whole‐Blood Units: Comparison between Three Additive Solutions: In vitro Studies

The in vitro effects of storage of platelets prepared from 4 or 6 pooled buffy coat (BC) units and stored in a platelet storage medium consisting of 30–40% of CPD plasma or alternatively half‐strength citrate CPD (0.5 CPD) plasma and 60–70% of different alternative platelet additive solutions (PASs) were evaluated. Measurements of mean platelet volume, pH, pO2, pCO2, bicarbonate, glucose, lactate, ATP, total adenine nucleotide content, extracellular lactate dehydrogenase or adenylate kinase activity, as markers for disintegration of platelets, and extracellular β‐thromboglobulin, as a marker for activation of platelets, were included in the in vitro studies. Previous studies indicated that a reduction of the citrate concentration from the standard 21 to 8 mmol/l is associated with a significant reduction of the consumption of glucose and production of lactate. Alternatively, similar effects can be obtained by the addition of acetate. In a preliminary paired study, the effects of different concentrations of acetate were tested. In an additional paired study, the effects of CPD plasma in combination with either saline or a PAS containing NaCl (115.5 mmol/l), citrate (10 mmol/l), and acetate (30 mmol/l), pH 7.2 (PAS‐2) were evaluated. 0.5CPD plasma in combination with either PAS‐2 or a nonacetate PAS (PAS‐1) were also tested. The storage of platelets in 0.5CPD plasma was used as a reference. The conclusions are: (1) A minimum acetate concentration of 30 mmol/l is needed to counteract the effects of citrate on the production of lactate. (2) pH and the bicarbonate buffering capacity are significantly better maintained in PAS‐2 than in PAS‐1. With PAS‐2, a significantly lower consumption of glucose and a significantly lower production of lactate are also observed. (3) When the storage period exceeds 5 days, PAS‐2 is superior to saline as plasma diluent with regard to the maintenance of pH. (4) A significantly lower consumption of glucose was noted in 0.5CPD plasma diluted with PAS‐2 as compared to undiluted 0.5CPD plasma. A significantly better maintenance of bicarbonate buffering capacity was also seen. (5) By comparing PAS‐2 in combination with either CPD or 0.5CPD, significantly lower values for pCO2 and consumption of glucose were observed with 0.5CPD. The levels of pH and pO2 are significantly higher with 0.5CPD. We conclude that PAS‐2 during 9‐day storage is associated with the most stable storage conditions with regard to the in vitro variables used in this study. The results suggest that at least 7 days storage of PCs in a medium consisting of 30% of either standard CPD or 0.5CPD plasma and 70% PAS‐2 is possible.

[1]  M. Blajchman,et al.  BACTERIAL CONTAMINATION OF CELLULAR BLOOD COMPONENTS , 1994, Vox sanguinis.

[2]  M. Lundholm,et al.  Novel automated microbial screening of platelet concentrates , 1994, APMIS : acta pathologica, microbiologica, et immunologica Scandinavica.

[3]  C. Högman,et al.  Half‐Strength Citrate CPD Combined with a New Additive Solution for Improved Storage of Red Blood Cells Suitable for Clinical Use , 1993, Vox sanguinis.

[4]  C. Högman,et al.  Half‐strength citrate CPD and new additive solutions for improved blood preservation. 2. The effect of storage at ambient temperature before component preparation and different means of supplying glucose to the red cells , 1993 .

[5]  E. Beutler.  Artificial preservatives for platelets , 1993, Transfusion.

[6]  S. Murphy,et al.  Roles of acetate and phosphate in the successful storage of platelet concentrates prepared with an acetate‐containing additive solution , 1993, Transfusion.

[7]  H. Gulliksson.  Storage of platelets in additive solutions: the effect of citrate and acetate in in vitro studies , 1993, Transfusion.

[8]  C. Högman,et al.  Platelet Concentrates in an Additive Solution Prepared from Pooled Buffy Coats , 1993, Vox sanguinis.

[9]  J. Payrat,et al.  Half‐strength citrate CPD and new additive solutions for improved blood preservation. I. Studies of six experimental solutions , 1993, Transfusion medicine.

[10]  A. Farrugia,et al.  Red Cell and Platelet Concentrates from Blood Collected into Half‐Strength Citrate Anticoagulant: Improved Maintenance of Red Cell 2,3‐Diphosphoglycerate in Half‐Citrate Red Cells , 1992, Vox sanguinis.

[11]  H. Gulliksson,et al.  Storage of platelets in additive solutions: a new method for storage using sodium chloride solution , 1992, Transfusion.

[12]  P. Rebulla,et al.  Role of acetate during platelet storage in a synthetic medium , 1992, Transfusion.

[13]  W. Heaton,et al.  Extended Storage of Platelets in an Artificial Medium with the Platelet Activation Inhibitors Prostaglandin E1 and Theophylline 1 , 1991, Vox sanguinis.

[14]  P. Withers,et al.  Alternative Fuels for Platelet Storage: A Metabolic Study , 1990, Vox sanguinis.

[15]  D. Riccardi,et al.  Evaluation of platelet concentrates prepared from buffy coats and stored in a glucose‐free crystalloid medium , 1989, Transfusion.

[16]  C. Prowse,et al.  Studies on the Procurement of Blood Coagulation Factor VIII , 1988, Vox Sanguinis.

[17]  S. Holme,et al.  Platelet Storage Lesion in Second‐Generation Containers: Correlation with Platelet ATP Levels , 1987, Vox sanguinis.

[18]  S. Holme,et al.  Improved in vivo and in vitro viability of platelet concentrates stored for seven days in a platelet additive solution , 1987, British journal of haematology.

[19]  C. Prowse,et al.  Studies on the Procurement of Blood Coagulation Factor VIII in vitro Studies on Blood Components Prepared in Half‐Strength Citrate Anticoagulant , 1987, Vox sanguinis.

[20]  H. Braine,et al.  Bacterial sepsis secondary to platelet transfusion: an adverse effect of extended storage at room temperature , 1986, Transfusion.

[21]  G. Rock,et al.  Platelet storage in a plasma‐free medium , 1985, Transfusion.

[22]  R. Aster,et al.  Extended storage of platelets in a new plastic container , 1985, Transfusion.

[23]  R. Coupland,et al.  The response of an acquired Factor V inhibitor to activated Factor IX concentrate , 1985, Transfusion.

[24]  S. Murphy,et al.  Platelet metabolism during storage of platelet concentrates at 22 degrees C. , 1984, Blood.

[25]  M. Marconi,et al.  Platelet concentrates from buffy coats: improved conditions for preparation and evaluation in routine clinical use. , 1993, Transfusion science.

[26]  C. Högman.  New trends in the preparation and storage of platelets. , 1992, Transfusion.