Relationship between p53 gene codon-72 polymorphisms and hypertrophic scar formation following caesarean section

The aim of the present study was to determine the relationship between p53 gene codon-72 polymorphisms and hypertrophic scar formation following caesarean section (CS). Blood samples from 260 female patients were collected one week following a CS for the detection of p53 gene polymorphisms using a molecular beacon-coupled quantitative polymerase chain reaction technique. Patients had follow-ups for 12–18 months to observe the scar formation. From these observations, the relationship between the p53 codon-72 polymorphisms and hypertrophic scar formation occurrence was investigated. Among the patients with the CCC/CCC genotype, nine patients had hypertrophic scars and 46 patients showed normal healing, which is a ratio of 0.19. However, the follow-up investigations indicated that the presence of a homozygous or heterozygous C-to-G alteration at the codon-72 site in gene p53 resulted in 13 patients with hypertrophic scars and 192 patients with normal healing, which is a ratio of 0.07. Therefore, these results indicate that patients with the CCC/CCC genotype had a higher risk of developing hypertrophic scars compared with that for patients with the CCC/CGC or CGC/CGC genotypes.

[1]  A. Bayat,et al.  Current understanding of molecular and cellular mechanisms in fibroplasia and angiogenesis during acute wound healing. , 2013, Journal of dermatological science.

[2]  Yingli Lu,et al.  Placenta mesenchymal stem cell accelerates wound healing by enhancing angiogenesis in diabetic Goto-Kakizaki (GK) rats. , 2013, Biochemical and biophysical research communications.

[3]  H. Bağcı,et al.  P53 codon 72 and HER2 codon 655 polymorphisms in Turkish breast cancer patients. , 2010, DNA and cell biology.

[4]  A. Santillo,et al.  Differential apoptosis markers in human keloids and hypertrophic scars fibroblasts , 2009, Molecular and Cellular Biochemistry.

[5]  Yubin Shi,et al.  Mechanical load initiates hypertrophic scar formation through decreased cellular apoptosis , 2007, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[6]  Q. Zhuang,et al.  [Association between p53 gene codon 72 polymorphism and keloid in Chinese population]. , 2007, Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery.

[7]  F. Lu,et al.  [p53 gene codon 72 polymorphism and susceptibility to keloid]. , 2005, Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery.

[8]  Hong-jie Duan,et al.  [Investigation of p53 gene mutations in keloids using PCR-SSCP]. , 2003, Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery.

[9]  G. Saed,et al.  Analysis of p53 gene mutations in keloids using polymerase chain reaction-based single-strand conformational polymorphism and DNA sequencing. , 1998, Archives of dermatology.

[10]  E. Middelkoop,et al.  Differential response of human adipose tissue-derived mesenchymal stem cells, dermal fibroblasts, and keratinocytes to burn wound exudates: potential role of skin-specific chemokine CCL27. , 2014, Tissue engineering. Part A.

[11]  Wim Ceelen,et al.  Surgery, wound healing, and metastasis: recent insights and clinical implications. , 2014, Critical reviews in oncology/hematology.

[12]  R. Kirchmair,et al.  Topical secretoneurin gene therapy accelerates diabetic wound healing by interaction between heparan-sulfate proteoglycans and basic FGF , 2013, Angiogenesis.

[13]  A. Grobbelaar,et al.  The role of the TGF-β family in wound healing, burns and scarring: a review. , 2012, International journal of burns and trauma.

[14]  Z. Salehi,et al.  TP53 codon 72 polymorphism and breast cancer in northern Iran. , 2009, Oncology research.

[15]  C. Kay,et al.  p53 tumour suppressor gene polymorphism is associated with recurrent implantation failure. , 2006, Reproductive biomedicine online.

[16]  N. Nakazawa,et al.  [Investigation of p53 polymorphism for genetic predisposition of keloid and hypertrophic scar]. , 2005, Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery.