Acetate utilization by the isolated perfused guinea-pig mammary gland

Summary Acetate uptake by isolated perfused guinea-pig mammary glands was approximately l·0 mg g−1 h−1 when perfusate acetate concentrations were in the physiological range (5–11 mg/100 ml plasma). At perfusate concentrations below this range (mean 3·62 mg/100 ml plasma) the uptake was not significantly different, but in one experiment in which the mean acetate concentration was 14·5 mg/100 ml plasma the uptake was markedly elevated. Radioactivity from Na [1-14C]acetate was incorporated into CO2 and milk and tissue fat, being largely present in C16 and C18 fatty acids. The guinea-pig is atypical of non-ruminants and similar to ruminants in respect of the blood concentration and mammary utilization of acetate.

[1]  S. Davis,et al.  Amino acid and glucose uptake by the isolated perfused guinea-pig mammary gland. , 1974, Quarterly journal of experimental physiology and cognate medical sciences.

[2]  R. Bickerstaffe,et al.  The metabolism of glucose, acetate, lipids and amino acids in lactating dairy cows , 1974, The Journal of Agricultural Science.

[3]  T. Mepham,et al.  Variation in the yield and composition of milk throughout lactation in the guinea pig (Cavia porcellus). , 1973, Comparative biochemistry and physiology. A, Comparative physiology.

[4]  I. Forsyth,et al.  The effects of hormones on milk-fat synthesis in mammary explants from pseudopregnant rabbits. , 1972, The Biochemical journal.

[5]  S. Lustick,et al.  Energetics in the opossum, Didelphis marsupialis virginiana , 1972 .

[6]  D. Noakes,et al.  The metabolism of glucose, acetate, palmitate, stearate and oleate in pigs , 1970, British Journal of Nutrition.

[7]  F. Hird,et al.  Concentrations and metabolism of volatile fatty acids in the fermentative organs of two species of kangaroo and the guinea-pig , 1970, British Journal of Nutrition.

[8]  J. Sutton,et al.  Fermentation in the rumen of cows given rations containing hay and flaked maize or rolled barley in widely different proportions , 1969, The Journal of Agricultural Science.

[9]  C. West,et al.  Mammary metabolism in lactating sows: arteriovenous differences of milk precursors and the mammary metabolism of [14C]glucose and [14C]acetate , 1969, British Journal of Nutrition.

[10]  E. A. Jones.  Section A. Recent developments in the biochemistry of the mammary gland , 1969, Journal of Dairy Research.

[11]  J. Linzell.  The magnitude and mechanisms of the uptake of milk precursors by the mammary gland , 1968, Proceedings of the Nutrition Society.

[12]  A. Kuksis,et al.  Molecular weight distributions of milk fat triglycerides from seven species. , 1967, Journal of lipid research.

[13]  J. F. Moran,et al.  Evaluation of quench correction in liquid scintillation counting by internal, automatic external, and channels' ratio standardization methods. , 1966, Analytical biochemistry.

[14]  O. Mcbride,et al.  Uptake of free fatty acids and chylomicron glycerides by guinea pig mammary gland in pregnancy and lactation. , 1964, Journal of lipid research.

[15]  E. Boyland,et al.  An enzyme from rat liver catalysing conjugations with glutathione. 2. Replacement of nitro groups. , 1963, The Biochemical journal.