AN RP-HPLC METHOD DEVELOPED FOR DETERMINATION OF BIFONAZOLE IN PHARMACEUTICAL FORMULATION

A Validated RP-HPLC method has been developed for estimation of Bifonazole in bulk and pharmaceutical cream. The method was developed using C18 column (250×4.6 mm; 5µm) with a mobile phase consisting of methanol and 0.1 M sodium acetate in ratio of 70:30 at P H 3, flow rate of 1 ml/min with retention time found 6.54 min. Detection was carried out at 252 nm with UV detector. The developed method was evaluated for various system suitability parameters and validated for linearity, accuracy, precision, LOD, LOQ as per ICH guidelines. The proposed method can be used for the estimation Bifonazole in their pharmaceutical dosage forms and no interference of excipients found in developed method.