Contact- and growth factor-dependent survival in a canine marrow-derived stromal cell line.

Cell-cell interactions and the presence of growth factors such as stem cell factor (SCF; or c-kit ligand) or interleukin-6 (IL-6) are involved in the proliferation and differentiation of the canine marrow-derived stromal cell line DO64. In the presence of SCF, stromal cells are induced to differentiate, but not to proliferate. In contrast, in the presence of IL-6, stromal cells are induced to proliferate rather than to differentiate in culture. Both SCF and IL-6 are produced by the stromal cells themselves and, thus, act as autocrine factors. In addition, DO64 cells also interact physically with each other in culture when grown under optimal culture conditions (70% to 90% cell confluence and in the presence of serum), thereby supporting proliferation and maintaining viability. Under conditions of lower cell density or low serum or growth factor concentrations in culture, DO64 cells tend to aggregate and form clusters. This increase in local cell concentration is associated with preservation of viability, presumably because of the accumulation of autocrine factors. If no signal, neither intercellular nor soluble, is provided, and DO64 cells are not able to reach a critical cell density or to produce sufficient factors in an autocrine fashion, the cells cease to proliferate and eventually die.

[1]  H. Deeg,et al.  Differentiation of canine bone marrow cells with hemopoietic characteristics from an adherent stromal cell precursor. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[2]  H. Deeg,et al.  Ultrastructural localization of stem cell factor in canine marrow-derived stromal cells. , 1995, Experimental hematology.

[3]  M. Horton,et al.  Apoptosis induced by inhibition of intercellular contact , 1994, The Journal of cell biology.

[4]  A. Wells,et al.  Cell movement elicited by epidermal growth factor receptor requires kinase and autophosphorylation but is separable from mitogenesis , 1994, The Journal of cell biology.

[5]  V. Broudy,et al.  Human umbilical vein endothelial cells display high-affinity c-kit receptors and produce a soluble form of the c-kit receptor. , 1994, Blood.

[6]  M. Ogawa,et al.  Differentiation and proliferation of hematopoietic stem cells. , 1993, Blood.

[7]  J. Voyvodic,et al.  Control of lens epithelial cell survival , 1993, The Journal of cell biology.

[8]  D. Williams,et al.  Support of human hematopoiesis in long-term bone marrow cultures by murine stromal cells selectively expressing the membrane-bound and secreted forms of the human homolog of the steel gene product, stem cell factor. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[9]  P. Quesenberry,et al.  Biologic significance of constitutive and subliminal growth factor production by bone marrow stroma. , 1992, Blood.

[10]  M. Raff,et al.  Social controls on cell survival and cell death , 1992, Nature.

[11]  B. Leclair,et al.  Expression of stem cell factor and c-kit mRNA in cultured endothelial cells, monocytes and cloned human bone marrow stromal cells (CFU-RF). , 1992, Experimental hematology.

[12]  L. Terstappen,et al.  Formation of haematopoietic microenvironment and haematopoietic stem cells from single human bone marrow stem cells , 1992, Nature.

[13]  K. Zsebo,et al.  Isolation of c-kit receptor-expressing cells from bone marrow, peripheral blood, and fetal liver: functional properties and composite antigenic profile. , 1991, Blood.

[14]  M. Moore.  Review: Stratton Lecture 1990. Clinical implications of positive and negative hematopoietic stem cell regulators. , 1991, Blood.

[15]  C. Eaves,et al.  Mechanisms that regulate the cell cycle status of very primitive hematopoietic cells in long-term human marrow cultures. II. Analysis of positive and negative regulators produced by stromal cells within the adherent layer. , 1991, Blood.

[16]  K. Zsebo,et al.  Identification, purification, and biological characterization of hematopoietic stem cell factor from buffalo rat liver-conditioned medium , 1990, Cell.

[17]  C. March,et al.  Identification of a ligand for the c-kit proto-oncogene , 1990, Cell.

[18]  D. Zipori.  Regulation of hemopoiesis by cytokines that restrict options for growth and differentiation. , 1990, Cancer cells.

[19]  K. Dorshkind,et al.  Regulation of hemopoiesis by bone marrow stromal cells and their products. , 1990, Annual review of immunology.

[20]  R. Storb,et al.  Long-term culture of canine bone marrow cells. , 1989, Experimental hematology.

[21]  J. Nemunaitis,et al.  Simian virus 40-transformed adherent cells from human long-term marrow cultures: cloned cell lines produce cells with stromal and hematopoietic characteristics. , 1987, Blood.

[22]  P. Simmons,et al.  Host origin of marrow stromal cells following allogeneic bone marrow transplantation , 1987, Nature.

[23]  F. Brodsky,et al.  Class II molecules of the major histocompatibility complex considered as differentiation markers. , 1986, Human immunology.

[24]  N. Drize,et al.  Origin of hemopoietic stromal progenitor cells in chimeras. , 1985, Experimental hematology.

[25]  A. Gown,et al.  Evidence for a stem cell common to hematopoiesis and its in vitro microenvironment: studies of patients with clonal hematopoietic neoplasia. , 1984, Leukemia research.

[26]  M. Tavassoli,et al.  Hemopoietic stromal microenvironment , 1983, American journal of hematology.

[27]  O. Witte,et al.  Long-term culture of B lymphocytes and their precursors from murine bone marrow. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[28]  L. Lajtha,et al.  Conditions controlling the proliferation of haemopoietic stem cells in vitro , 1977, Journal of cellular physiology.