Ribonuclease H1 maps to chromosome 2 and has at least three pseudogene loci in the human genome.

We have analyzed the genomic structure of ribonuclease H1 (RNase H1) loci in the human genome. Human PAC library screening combined with database searches indicated that several loci are present. The transcribed gene is localized on chromosome 2p25. This was confirmed by RNA analysis of a monochromosomal hybrid cell line that expressed human chromosome 2. These data contradict a previous report, as well as the current Human Genome Project (HGP) annotation, which had placed the gene on chromosome 17p11.2. This location represents a pseudogene. Another highly similar pseudogene is present at a separate locus located more distal on chromosome 17p, while a third pseudogene is localized on chromosome 1q.

[1]  P. Devilee,et al.  In situ hybridization as a tool to study numerical chromosome aberrations in solid bladder tumors , 2004, Histochemistry.

[2]  T. Okazaki,et al.  Function of RNase H in DNA replication revealed by RNase H defective mutants of Escherichia coli , 2004, Molecular and General Genetics MGG.

[3]  Frank Baas,et al.  The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides. , 2002, European journal of biochemistry.

[4]  R. Crouch,et al.  RNase H1 of Saccharomyces cerevisiae: methods and nomenclature. , 2001, Methods in enzymology.

[5]  D. Ledbetter,et al.  An optimized set of human telomere clones for studying telomere integrity and architecture. , 2000, American journal of human genetics.

[6]  F. Baas,et al.  Polymorphisms in the large subunit of human RNA polymerase II as target for allele-specific inhibition. , 2000, Nucleic acids research.

[7]  S. Crooke,et al.  Molecular mechanisms of action of antisense drugs. , 1999, Biochimica et biophysica acta.

[8]  M. Denis,et al.  Gene walking by PCR amplification of short fragments from Taq DNA polymerase--modified P1 plasmid DNA and TA cloning. , 1999, BioTechniques.

[9]  M. Itaya,et al.  Identification of the genes encoding Mn2+-dependent RNase HII and Mg2+-dependent RNase HIII from Bacillus subtilis: classification of RNases H into three families. , 1999, Biochemistry.

[10]  K. Holzmann,et al.  Cloning, Subcellular Localization and Functional Expression of Human RNase HII , 1998, Biological chemistry.

[11]  R. Crouch,et al.  Cloning, expression, and mapping of ribonucleases H of human and mouse related to bacterial RNase HI. , 1998, Genomics.

[12]  P. Frank,et al.  Cloning of the cDNA encoding the large subunit of human RNase HI, a homologue of the prokaryotic RNase HII. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[13]  S. Crooke,et al.  Molecular cloning and expression of cDNA for human RNase H. , 1998, Antisense & nucleic acid drug development.

[14]  R. Bambara,et al.  Enzymes and Reactions at the Eukaryotic DNA Replication Fork* , 1997, The Journal of Biological Chemistry.

[15]  L. D. de Vries,et al.  PRENATAL AND POSTNATAL INVESTIGATION OF A CASE WITH MILLER–DIEKER SYNDROME DUE TO A FAMILIAL CRYPTIC TRANSLOCATION t(17;20) (p13.3;q13.3) DETECTED BY FLUORESCENCE IN SITU HYBRIDIZATION , 1997, Prenatal diagnosis.

[16]  J. Turchi,et al.  Enzymatic completion of mammalian lagging-strand DNA replication. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[17]  J. Toulmé,et al.  Purification and characterization of human ribonuclease HII. , 1994, Nucleic acids research.

[18]  Y. Nakamura,et al.  High-resolution cytogenetic mapping of 342 new cosmid markers including 43 RFLP markers on human chromosome 17 by fluorescence in situ hybridization. , 1993, Genomics.

[19]  P F Little,et al.  High-resolution localization of 69 potential human zinc finger protein genes: a number are clustered. , 1992, Genomics.

[20]  U. Wintersberger.  Ribonucleases H of retroviral and cellular origin. , 1990, Pharmacology & therapeutics.

[21]  H. Willard,et al.  Structure, organization, and sequence of alpha satellite DNA from human chromosome 17: evidence for evolution by unequal crossing-over and an ancestral pentamer repeat shared with the human X chromosome , 1986, Molecular and cellular biology.

[22]  J. Rech,et al.  Isolation and characterization of two types of ribonucleases H in Krebs II ascites cells. , 1979, The Journal of biological chemistry.

[23]  J. Hindley,et al.  Cloning of human satellite III DNA: different components are on different chromosomes. , 1979, Nucleic acids research.

[24]  P. Hausen,et al.  Distinct ribonuclease H activities in calf thymus. , 1975, European journal of biochemistry.

[25]  P. Hausen,et al.  Ribonuclease H. An enzyme degrading the RNA moiety of DNA-RNA hybrids. , 1970, European Journal of Biochemistry.

[26]  P. Hausen,et al.  Enzyme from Calf Thymus Degrading the RNA Moiety of DNA-RNA Hybrids: Effect on DNA-Dependent RNA Polymerase , 1969, Science.