Electrohypersensitivity: State-of-the-art of a Functional Impairment the Biological Effects of Weak Electromagnetic Fields

Recently, a new category of persons, claiming to suffer from exposure to electromagnetic fields, has been described in the literature. In Sweden, electrohypersensitivity (EHS) is an officially fully recognized functional impairment (i.e., it is not regarded as a disease). Survey studies show that somewhere between 230,000–290,000 Swedish men and women report a variety of symptoms when being in contact with electromagnetic field (EMF) sources. The aim of our studies has been to investigate possible alterations, in the cellular and neuronal systems of these person' skin. As controls, age- and sex-matched persons, without any subjective or clinical symptoms or dermatological history, served. Immunohistochemistry using antisera to the previously characterized marker substances of interest has been utilized. In summary, it is evident from our preliminary data that various alterations are present in the electrohypersensitive person' skin. In view of recent epidemiological studies, pointing to a correlation between long-term exposure from power-frequent magnetic fields or microwaves and cancer, our data ought to be taken seriously and further analyzed.

[1]  M. Hilliges,et al.  Intraepidermal nerves in human skin: PGP 9.5 immunohistochemistry with special reference to the nerve density in skin from different body regions. , 1999, Journal of the peripheral nervous system.

[2]  M. A. Barracco,et al.  Lectins, as non-self recognition factors in crustaceans , 2000 .

[3]  M. Cajaraville,et al.  Oxygen radicals production and actin filament disruption in bivalve haemocytes treated with benzo(a)pyrene. , 2002, Marine environmental research.

[4]  K. Croen,et al.  Evidence for antiviral effect of nitric oxide. Inhibition of herpes simplex virus type 1 replication. , 1993, The Journal of clinical investigation.

[5]  W. R. Adey,et al.  Sensitivity of calcium binding in cerebral tissue to weak environmental electric fields oscillating at low frequency. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[6]  The opiate receptors. , 1978 .

[7]  J. Pearce,et al.  THE 1999 LONG ISLAND SOUND LOBSTER MORTALITY EVENT: FINDINGS OF THE COMPREHENSIVE RESEARCH INITIATIVE , 2009 .

[8]  N. Grosman,et al.  Influence of pyrethroids and piperonyl butoxide on the Ca(2+)-ATPase activity of rat brain synaptosomes and leukocyte membranes. , 2005, International immunopharmacology.

[9]  F. Murad Nitric oxide signaling: would you believe that a simple free radical could be a second messenger, autacoid, paracrine substance, neurotransmitter, and hormone? , 1998, Recent progress in hormone research.

[10]  A. Gorren,et al.  The reaction of nitric oxide with copper proteins and the photodissociation of copper-NO complexes. , 1987, Biochimica et biophysica acta.

[11]  M. F. Goy,et al.  Expression of nitric oxide synthase and nitric oxide‐sensitive guanylate cyclase in the crustacean cardiac ganglion , 2002, The Journal of comparative neurology.

[12]  Olle Johansson,et al.  Personal digital assistant (PDA) cell phone units produce elevated extremely‐low frequency electromagnetic field emissions , 2007 .

[13]  R. Valente,et al.  SEASONAL VARIATIONS IN SEDIMENT AND BOTTOM WATER CHEMISTRY OF WESTERN LONG ISLAND SOUND: IMPLICATIONS FOR LOBSTER MORTALITY , 2009 .

[14]  G. Cavallo,et al.  Effect of morphine on resistance to infection. , 1983, The Journal of infectious diseases.

[15]  R. Goodman,et al.  Stimulation of the stress response by low-frequency electromagnetic fields: possibility of direct interaction with DNA , 2000 .

[16]  Olle Johansson,et al.  The effect of extremely low-frequency electromagnetic fields on skin and thyroid amine- and peptide-containing cells in rats: an immunohistochemical and morphometrical study. , 2005, Environmental research.

[17]  M. Cajaraville,et al.  Comparative effects of cadmium, copper, paraquat and benzo[a]pyrene on the actin cytoskeleton and production of reactive oxygen species (ROS) in mussel haemocytes. , 2003, Toxicology in vitro : an international journal published in association with BIBRA.

[18]  E. Marder,et al.  Nitric Oxide Inhibits the Rate and Strength of Cardiac Contractions in the Lobster Homarus americanus by Acting on the Cardiac Ganglion , 2004, The Journal of Neuroscience.

[19]  G. Stefano,et al.  Endogenous morphine levels increase in molluscan neural and immune tissues after physical trauma , 1999, Brain Research.

[20]  K. Utsumi,et al.  Oxygen-dependent regulation of mitochondrial energy metabolism by nitric oxide. , 1995, Archives of biochemistry and biophysics.

[21]  T. Bilfinger,et al.  Estradiol coupling to human monocyte nitric oxide release is dependent on intracellular calcium transients: evidence for an estrogen surface receptor. , 1999, Journal of immunology.

[22]  D. Henshaw,et al.  Increased exposure to pollutant aerosols under high voltage power lines. , 1999, International journal of radiation biology.

[23]  M. F. Goy,et al.  Identification of Nitric Oxide‐Sensitive and ‐Insensitive Forms of Cytoplasmic Guanylate Cyclase , 1997, Journal of neurochemistry.

[24]  K. Jokela,et al.  ASSESSMENT OF THE MAGNETIC FIELD EXPOSURE DUE TO THE BATTERY CURRENT OF DIGITAL MOBILE PHONES , 2004, Health physics.

[25]  C. Szabó Alterations in nitric oxide production in various forms of circulatory shock. , 1995, New Horizons.

[26]  T. Bilfinger,et al.  Human granulocytes contain an opiate alkaloid-selective receptor mediating inhibition of cytokine-induced activation and chemotaxis. , 1995, Journal of immunology.

[27]  T. Bilfinger,et al.  Antagonism of LPS and IFN-gamma induction of iNOS in human saphenous vein endothelium by morphine and anandamide by nitric oxide inhibition of adenylate cyclase. , 1998, Journal of cardiovascular pharmacology.

[28]  Olle Johansson,et al.  Malignant melanoma of the skin - not a sunshine story! , 2004, Medical science monitor : international medical journal of experimental and clinical research.

[29]  Martin Blank,et al.  Regulating genes with electromagnetic response elements , 2001, Journal of cellular biochemistry.

[30]  C. Szabó Physiological and pathophysiological roles of nitric oxide in the central nervous system , 1996, Brain Research Bulletin.

[31]  Lennart Hardell,et al.  Cellular and Cordless Telephone Use and the Association with Brain Tumors in Different Age Groups , 2004, Archives of environmental health.

[32]  L. Ballarin,et al.  Tributyltin induces cytoskeletal alterations in the colonial ascidian Botryllus schlosseri phagocytes via interaction with calmodulin. , 2000, Aquatic toxicology.

[33]  M. Hilliges,et al.  A screening of skin changes, with special emphasis on neurochemical marker antibody evaluation, in patients claiming to suffer from “screen dermatitis” as compared to normal healthy controls , 1996, Experimental dermatology.

[34]  L. Cerenius,et al.  Role of the prophenoloxidase-activating system in invertebrate immunity. , 1998, Current opinion in immunology.

[35]  C. Nathan,et al.  Regulation of biosynthesis of nitric oxide. , 1994, The Journal of biological chemistry.

[36]  V. Smith,et al.  Comparison of antibacterial activity in the hemocytes of different crustacean species. , 1995, Comparative biochemistry and physiology. Part A, Physiology.

[37]  W. Donaldson,et al.  Impairment of calcium homeostasis by hexachlorobenzene (HCB) exposure in Fischer 344 rats. , 1988, Journal of toxicology and environmental health.

[38]  T. Bilfinger,et al.  Effect of prolonged exposure to morphine on responsiveness of human and invertebrate immunocytes to stimulatory molecules , 1995, Journal of Neuroimmunology.

[39]  V. Kanji,et al.  Pesticide induced changes of nitric oxide synthase in rat brain in vitro. , 1999, Drug and chemical toxicology.

[40]  I Rovira,et al.  Nitric oxide , 2021, Reactions Weekly.

[41]  Ö. Hallberg,et al.  FM Broadcasting Exposure Time and Malignant Melanoma Incidence , 2005 .

[42]  C. Breton,et al.  Estradiol-stimulated nitric oxide release in human granulocytes is dependent on intracellular calcium transients: evidence of a cell surface estrogen receptor , 2000 .

[43]  Ö. Hallberg,et al.  Mobile Handset Output Power and Health , 2004 .

[44]  Skin changes in patients claiming to suffer from “screen dermatitis”: a two‐case open‐field provocation study , 1994, Experimental dermatology.

[45]  O. Johansson,et al.  A theoretical model based upon mast cells and histamine to explain the recently proclaimed sensitivity to electric and/or magnetic fields in humans. , 2000, Medical hypotheses.

[46]  L. Chernomordik,et al.  Voltage-induced nonconductive pre-pores and metastable single pores in unmodified planar lipid bilayer. , 2001, Biophysical journal.

[47]  T. Bilfinger,et al.  Basal nitric oxide limits immune, nervous and cardiovascular excitation: human endothelia express a mu opiate receptor , 2000, Progress in Neurobiology.

[48]  G. Fricchione,et al.  Opioid and opiate immunoregulatory processes. , 1996, Critical reviews in immunology.

[49]  W. R. Adey,et al.  EFFECTS OF MODULATED VHF FIELDS ON THE CENTRAL NERVOUS SYSTEM * , 1975, Annals of the New York Academy of Sciences.

[50]  Ö. Hallberg,et al.  ALZHEIMER MORTALITY - WHY DOES IT INCREASE SO FAST IN SPARSELY POPULATED AREAS? , 2005 .

[51]  G. Stefano,et al.  Endogenous morphine and related opiates, a new class of chemical messengers. , 1994, Advances in neuroimmunology.

[52]  Dimitris Michailakis,et al.  Standard Rules on the Equalization of Opportunities for Persons with Disabilities , 1995, Human Rights and Disabled Persons.

[53]  S. Hosztafi,et al.  Endogenous morphine. , 1995, Pharmacological research.

[54]  G. Fricchione,et al.  Morphine-induced conformational changes in human monocytes, granulocytes, and endothelial cells and in invertebrate immunocytes and microglia are mediated by nitric oxide. , 1996, Journal of immunology.

[55]  M. Weisenseel,et al.  Weak AC-electric fields promote root growth and ER abundance of root cap cells 1 The memory of Dr. H , 1998 .

[56]  N. Wertheimer,et al.  Electrical wiring configurations and childhood cancer. , 1979, American journal of epidemiology.

[57]  T. Eisenstein,et al.  Morphine treatment in vitro or in vivo decreases phagocytic functions of murine macrophages. , 1993, Life sciences.

[58]  S. Imaoka,et al.  Hepatic cytochrome P450 is directly inactivated by nitric oxide, not by inflammatory cytokines, in the early phase of endotoxemia. , 1999, Journal of hepatology.

[59]  S. Shak,et al.  Spreading of human neutrophils is immediately preceded by a large increase in cytoplasmic free calcium. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[60]  K. H. Mild,et al.  Use of cellular telephones and the risk for brain tumours: A case-control study. , 1999, International journal of oncology.

[61]  Dimitris J. Panagopoulos,et al.  Cell death induced by GSM 900-MHz and DCS 1800-MHz mobile telephony radiation. , 2007, Mutation research.

[62]  K. Hahm,et al.  Suppression of inducible nitric oxide synthase and tumor necrosis factor-alpha expression by 4-nonylphenol in macrophages. , 2002, Biochemical and biophysical research communications.

[63]  G. Stefano Autoimmunovascular regulation: morphine and ancondamide and ancondamide stimulated nitric oxide release , 1998, Journal of Neuroimmunology.

[64]  O. Johansson,et al.  Histological characteristics of cutaneous and thyroid mast cell populations in male rats exposed to power-frequency electromagnetic fields , 2005, International journal of radiation biology.

[65]  I. Cooke,et al.  The distribution of NADPH diaphorase activity and immunoreactivity to nitric oxide synthase in the nervous system of the pulmonate mollusc Helix aspersa , 1994, Cell and Tissue Research.

[66]  E. Matthews Calcium and membrane permeability. , 1986, British medical bulletin.

[67]  A. Goldsworthy Effects of Electrical and Electromagnetic Fields on Plants and Related Topics , 2006 .

[68]  Kjell Hansson Mild,et al.  Case-Control Study on Cellular and Cordless Telephones and the Risk for Acoustic Neuroma or Meningioma in Patients Diagnosed 2000–2003 , 2005, Neuroepidemiology.

[69]  P. Keyser,et al.  Crustacean haemocytes and haematopoiesis , 2000 .

[70]  L. S. Kinney,et al.  Effects of ELF fields on calcium-ion efflux from brain tissue in vitro. , 1982, Radiation research.

[71]  T. Menges,et al.  Morphine Inhibits NF-&kgr;B Nuclear Binding in Human Neutrophils and Monocytes by a Nitric Oxide–dependent Mechanism , 2000, Anesthesiology.

[72]  G. Brown,et al.  Reversible binding and inhibition of catalase by nitric oxide. , 1995, European journal of biochemistry.

[73]  Olle Johansson,et al.  Personal digital assistant (PDA) cell phone units produce elevated extremely‐low frequency electromagnetic field emissions , 2007, Bioelectromagnetics.

[74]  H. Berg,et al.  Proliferation response of yeast saccharomyces cerevisiae on electromagnetic field parameters , 1997 .

[75]  Timothy J Shafer,et al.  Effects of pyrethroids on voltage-sensitive calcium channels: a critical evaluation of strengths, weaknesses, data needs, and relationship to assessment of cumulative neurotoxicity. , 2004, Toxicology and applied pharmacology.

[76]  P. F. Baker,et al.  The effects of nitroprusside and putative agonists on guanylate cyclase activity in squid giant axons. , 1988, Biochimica et biophysica acta.

[77]  B. Ha Stabilization and destabilization of cell membranes by multivalent ions. , 2001, Physical review. E, Statistical, nonlinear, and soft matter physics.

[78]  So young Lee,et al.  Early events in crustacean innate immunity. , 2002, Fish & shellfish immunology.

[79]  Örjan Hallberg,et al.  Melanoma Incidence and Frequency Modulation (FM) Broadcasting , 2002, Archives of environmental health.

[80]  J. Pérez-Castrillón,et al.  Opioids depress in vitro human monocyte chemotaxis. , 1992, Immunopharmacology.

[81]  Örjan Hallberg,et al.  Cancer trends during the 20th century , 2002 .

[82]  K. Söderhäll,et al.  The proPO and clotting system in crustaceans , 2000 .

[83]  C. Szabó,et al.  Evaluation of the relative contribution of nitric oxide and peroxynitrite to the suppression of mitochondrial respiration in immunostimulated macrophages using a manganese mesoporphyrin superoxide dismutase mimetic and peroxynitrite scavenger , 1996, FEBS letters.

[84]  Franz Adlkofer,et al.  Non-thermal DNA breakage by mobile-phone radiation (1800 MHz) in human fibroblasts and in transformed GFSH-R17 rat granulosa cells in vitro. , 2005, Mutation research.

[85]  D. Henshaw,et al.  Corona ions from powerlines and increased exposure to pollutant aerosols. , 1999, International journal of radiation biology.

[86]  D. Desaiah,et al.  In vitro effects of organophosphorus compounds on calmodulin activity , 1991, Journal of applied toxicology : JAT.

[87]  C. Breton,et al.  Estradiol-stimulated nitric oxide release in human granulocytes is dependent on intracellular calcium transients: evidence of a cell surface estrogen receptor. , 2000, Blood.

[88]  M. Hilliges,et al.  Ultrastructural evidence for nerve fibers within all vital layers of the human epidermis. , 1995, The Journal of investigative dermatology.

[89]  S. Abramson,et al.  Nitric oxide stimulates the ADP-ribosylation of actin in human neutrophils. , 1993, Biochemical and biophysical research communications.

[90]  B Hocking,et al.  Mobile phone use and risk of acoustic neuroma , 2006, British Journal of Cancer.

[91]  H. Berg,et al.  Extremely Low Frequency Electromagnetic Fields: The Question of Cancer , 1991 .

[92]  S. Moncada,et al.  Nitric oxide: physiology, pathophysiology, and pharmacology. , 1991, Pharmacological reviews.

[93]  B. McLeod,et al.  Effects of CR-tuned 60 Hz magnetic fields on sprouting and early growth of Raphanus sativus , 1993 .

[94]  A. van Dorsselaer,et al.  Penaeidins, a New Family of Antimicrobial Peptides Isolated from the Shrimp Penaeus vannamei (Decapoda)* , 1997, The Journal of Biological Chemistry.

[95]  G. Stefano,et al.  The American lobster, Homarus americanus, contains morphine that is coupled to nitric oxide release in its nervous and immune tissues: Evidence for neurotransmitter and hormonal signaling. , 2005, Neuro endocrinology letters.

[96]  G. Stefano,et al.  A neuroimmunoregulatory-like mechanism responding to stress in the marine bivalve Mytilus edulis , 1990, Brain, Behavior, and Immunity.

[97]  T. Bilfinger,et al.  Human neutrophil and macrophage chemokinesis induced by cardiopulmonary bypass: Loss of DAME and IL-1 chemotaxis , 1993, Journal of Neuroimmunology.

[98]  H. Kosterlitz,et al.  Opiate receptors , 1976, Nature.

[99]  T. Jernberg,et al.  Protein gene product 9.5-immunoreactive nerve fibres and cells in human skin , 1990, Cell and Tissue Research.

[100]  T. Bilfinger,et al.  Presence of the mu3 opiate receptor in endothelial cells. Coupling to nitric oxide production and vasodilation. , 1995, The Journal of biological chemistry.

[101]  C. Nathan,et al.  Role of transcription factor NF-kappa B/Rel in induction of nitric oxide synthase. , 1994, The Journal of biological chemistry.

[102]  J. Polak,et al.  Nitric oxide (NO) synthase immunoreactivity in the starfish Marthasterias glacialis , 1994, Cell and Tissue Research.

[103]  Olle Johansson,et al.  Cutaneous mast cells are altered in normal healthy volunteers sitting in front of ordinary TVs/PCs – results from open‐field provocation experiments , 2001, Journal of cutaneous pathology.

[104]  A. Stead,et al.  Morphometric analysis of osteosclerotic bone resulting from hexachlorobenzene exposure. , 1990, Journal of toxicology and environmental health.

[105]  M. Muraji,et al.  Primary root growth rate of Zea mays seedlings grown in an alternating magnetic field of different frequencies , 1998 .

[106]  G. Stefano,et al.  Morphine coupling to invertebrate immunocyte nitric oxide release is dependent on intracellular calcium transients. , 1999, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology.

[107]  B. Brownawell,et al.  ACUTE TOXICITY OF RESMETHRIN, MALATHION AND METHOPRENE TO LARVAL AND JUVENILE AMERICAN LOBSTERS (HOMARUS AMERICANUS) AND ANALYSIS OF PESTICIDE LEVELS IN SURFACE WATERS AFTER SCOURGE™, ANVIL™AND ALTOSID™APPLICATION , 2009 .

[108]  A. Calabrese,et al.  ii The Long Island Sound Lobster Research Initiative , 2003 .

[109]  M. Wilson,et al.  The reactions of copper proteins with nitric oxide. , 1999, Biochimica et biophysica acta.

[110]  S. Snyder,et al.  Opiate Receptors , 1988, Encyclopedia of Behavioral Medicine.

[111]  G. Stefano,et al.  Rebound from Nitric Oxide Inhibition Triggers Enhanced Monocyte Activation and Chemotaxis1 , 2000, The Journal of Immunology.

[112]  J. Truman,et al.  The NO/cGMP pathway and the development of neural networks in postembryonic lobsters. , 1998, Journal of neurobiology.

[113]  A. Lackie Invertebrate immunity , 1980, Parasitology.

[114]  S. Moncada,et al.  The L-arginine-nitric oxide pathway. , 1993, The New England journal of medicine.

[115]  P. Bulet,et al.  Penaeidins, antimicrobial peptides of shrimp: a comparison with other effectors of innate immunity , 2000 .

[116]  J. Hose,et al.  Defense functions of granulocytes in the ridgeback prawn , 1989 .

[117]  O. Johansson,et al.  Skin changes in “screen dermatitis” versus classical UV‐ and ionizing irradiation‐related damage — similarities and differences , 1997, Experimental dermatology.

[118]  T. Bilfinger,et al.  NF- κ B, nitric oxide and opiate signaling☆ , 2000 .

[119]  O. Johansson,et al.  Light and electron microscopic study of the thyroid gland in rats exposed to power-frequency electromagnetic fields , 2006, Journal of Experimental Biology.

[120]  G. Fricchione,et al.  The blueprint for stress can be found in invertebrates. , 2002, Neuro endocrinology letters.