Noninvasive In Vivo Monitoring of Bone Architecture Alterations in Hindlimb‐Unloaded Female Rats Using Novel Three‐Dimensional Microcomputed Tomography

We tested a novel microcomputed tomograph designed to longitudinally and noninvasively monitor bone alterations in hindlimb‐unloaded female rats at a resolution of 26 μm over a period of 3 weeks. This prototype has a potential to detect three‐dimensional trabecular microarchitectural changes induced by growth and unloading.

[1]  Laurence Vico,et al.  High-Resolution Three-Dimensional Micro-Computed Tomography Detects Bone Loss and Changes in Trabecular Architecture Early: Comparison with DEXA and Bone Histomorphometry in a Rat Model of Disuse Osteoporosis , 2002, Investigative radiology.

[2]  M. Sosa,et al.  Effect of two forms of alendronate administration upon bone mass after two years of treatment. , 2002, Journal of clinical densitometry : the official journal of the International Society for Clinical Densitometry.

[3]  P. Meunier,et al.  Alendronate increases bone strength by increasing the mean degree of mineralization of bone tissue in osteoporotic women. , 2000, Bone.

[4]  S. Holm.  A Simple Sequentially Rejective Multiple Test Procedure , 1979 .

[5]  W. J. Whitehouse.  The quantitative morphology of anisotropic trabecular bone , 1974, Journal of microscopy.

[6]  Riggs Bl.  A new option for treating osteoporosis. , 1990 .

[7]  P Rüegsegger,et al.  In vivo micro tomography. , 2001, Advances in experimental medicine and biology.

[8]  E. Morey-Holton,et al.  Skeletal response to simulated weightlessness: a comparison of suspension techniques. , 1987, Aviation, space, and environmental medicine.

[9]  P. Rüegsegger,et al.  A new method for the model‐independent assessment of thickness in three‐dimensional images , 1997 .

[10]  M. Ito,et al.  Differences of three-dimensional trabecular microstructure in osteopenic rat models caused by ovariectomy and neurectomy. , 2002, Bone.

[11]  H. Gundersen,et al.  Quantification of connectivity in cancellous bone, with special emphasis on 3-D reconstructions. , 1993, Bone.

[12]  S. Majumdar,et al.  Magnetic resonance imaging of trabecular bone structure in the distal radius: Relationship with X-ray tomographic microscopy and biomechanics , 2005, Osteoporosis International.

[13]  D. Mitton,et al.  High-Resolution Computed Tomography for Architectural Characterization of Human Lumbar Cancellous Bone: Relationships with Histomorphometry and Biomechanics , 1999, Osteoporosis International.

[14]  Ming Ding,et al.  Age‐related variations in the microstructure of human tibial cancellous bone , 2002, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[15]  T. Yamamuro,et al.  Microstructural changes of osteopenic trabeculae in the rat. , 1991, Bone.

[16]  A. Parfitt.  Implications of architecture for the pathogenesis and prevention of vertebral fracture. , 1992, Bone.

[17]  P. Rüegsegger,et al.  Morphometric analysis of human bone biopsies: a quantitative structural comparison of histological sections and micro-computed tomography. , 1998, Bone.

[18]  William E. Lorensen,et al.  Marching cubes: A high resolution 3D surface construction algorithm , 1987, SIGGRAPH.

[19]  P. Rüegsegger,et al.  Direct Three‐Dimensional Morphometric Analysis of Human Cancellous Bone: Microstructural Data from Spine, Femur, Iliac Crest, and Calcaneus , 1999, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[20]  TOR Hildebrand,et al.  Quantification of Bone Microarchitecture with the Structure Model Index. , 1997, Computer methods in biomechanics and biomedical engineering.

[21]  M. Kleerekoper,et al.  Relationships between surface, volume, and thickness of iliac trabecular bone in aging and in osteoporosis. Implications for the microanatomic and cellular mechanisms of bone loss. , 1983, The Journal of clinical investigation.

[22]  S. Majumdar,et al.  The Temporal Changes of Trabecular Architecture in Ovariectomized Rats Assessed by MicroCT , 2001, Osteoporosis International.

[23]  P. Rüegsegger,et al.  Calibration of trabecular bone structure measurements of in vivo three-dimensional peripheral quantitative computed tomography with 28-microm-resolution microcomputed tomography. , 1999, Bone.

[24]  L. Vico,et al.  Bone tissue response to four-month antiorthostatic bedrest: A bone histomorphometric study , 1992, Calcified Tissue International.

[25]  R. Mann,et al.  Characterization of microstructural anisotropy in orthotropic materials using a second rank tensor , 1984 .

[26]  Noboru Kikuchi,et al.  The Mechanical and Remodeling Properties of Trabecular Bone , 1990 .

[27]  G. Rodan,et al.  Osteopenia in the immobilized rat hind limb is associated with increased bone resorption and decreased bone formation. , 1989, Bone.