Comparative Study on Polysaccharide from Sipunculus nudus by Different Extraction Process

Objective To study enzyme treatment,alkali treatment and a combination of the two processes for the extraction of Sipunculus nudus polysaccharide and compare these three methods.Methods The content of polysaccharide was determined by colorimetry using anthrone-sulfuric acid method,and the yield of polysaccharides was obtained by gravimetric method.Results The results indicated that the yield of polysaccharides was 0.60%(enzyme method),1.47%(alkali method) and 0.20%(combined method),and the content of polysaccharide was 21.83%、87.30% and 36.40% respectively.Conclusion The extraction process of alkali method is feasible and is more efficient than the other two methods.