The ACB technique: a biomagentic tool for monitoring gastrointestinal contraction directly from smooth muscle in dogs

The aim of this paper was to verify whether AC biosusceptometry (ACB) is suitable for monitoring gastrointestinal (GI) contraction directly from smooth muscle in dogs, comparing with electrical recordings simultaneously. All experiments were performed in dogs with magnetic markers implanted under the serosa of the right colon and distal stomach, and their movements were recorded by ACB. Monopolar electrodes were implanted close to the magnetic markers and their electric potentials were recorded by electromyography (EMG). The effects of neostigmine, hyoscine butylbromide and meal on gastric and colonic parameters were studied. The ACB signal from the distal stomach was very similar to EMG; in the colonic recordings, however, within the same low-frequency band, ACB and EMG signals were characterized by simultaneity or a widely changeable frequency profile with time. ACB recordings were capable of demonstrating the changes in gastric and colonic motility determined by pharmacological interventions as well as by feeding. Our results reinforce the importance of evaluating the mechanical and electrical components of motility and show a temporal association between them. ACB and EMG are complementary for studying motility, with special emphasis on the colon. ACB offers an accurate method for monitoring in vivo GI motility.

[1]  O. Baffa,et al.  Gastric motility evaluated by electrogastrography and alternating current biosusceptometry in dogs , 2008, Physiological measurement.

[2]  P. Dinning,et al.  Proximal colonic propagating pressure waves sequences and their relationship with movements of content in the proximal human colon , 2008, Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society.

[3]  K. Stafford,et al.  High-definition spatiotemporal mapping of contractile activity in the isolated proximal colon of the rabbit , 2008, Journal of Comparative Physiology B.

[4]  L. Ver Donck,et al.  Mapping slow waves and spikes in chronically instrumented conscious dogs: automated on-line electrogram analysis , 2008, Medical & Biological Engineering & Computing.

[5]  A. Sanjeevi,et al.  Gastric motility , 2007, Current opinion in gastroenterology.

[6]  O. Baffa,et al.  Scintigraphic validation of AC Biosusceptometry to study the gastric motor activity and the intragastric distribution of food in humans , 2007, Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society.

[7]  O. Baffa,et al.  A novel biomagnetic approach to study caecocolonic motility in humans , 2006, Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society.

[8]  A. Irimia,et al.  Biomagnetic characterization of spatiotemporal parameters of the gastric slow wave , 2006, Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society.

[9]  Jiande D. Z. Chen,et al.  Experimental gastric dysrhythmias and its correlation with in vivo gastric muscle contractions. , 2006, World journal of gastroenterology.

[10]  L. Ver Donck,et al.  Mapping slow waves and spikes in chronically instrumented conscious dogs: implantation techniques and recordings , 2006, Medical and Biological Engineering and Computing.

[11]  O. Baffa,et al.  AC biosusceptometry in the study of drug delivery. , 2005, Advanced drug delivery reviews.

[12]  O. Baffa,et al.  Measurement of gastric contraction activity in dogs by means of AC biosusceptometry. , 2003, Physiological measurement.

[13]  O. Baffa,et al.  Oesophageal transit time evaluated by a biomagnetic method , 1998, Physiological measurement.

[14]  K. Sanders,et al.  Control of motility patterns in the human colonic circular muscle layer by pacemaker activity , 1998, The Journal of physiology.

[15]  W. Orr,et al.  Effects of meal volume and composition on gastric myoelectrical activity. , 1998, American journal of physiology. Gastrointestinal and liver physiology.

[16]  C. Hidalgo,et al.  Cyclic ADP-ribose activates caffeine-sensitive calcium channels from sea urchin egg microsomes. , 1998, The American journal of physiology.

[17]  O. Baffa,et al.  A novel biomagnetic method to study gastric antral contractions. , 1997, Physics in medicine and biology.

[18]  O. Baffa,et al.  Evaluation of a biomagnetic technique for measurement of orocaecal transit time. , 1996, European journal of gastroenterology & hepatology.

[19]  N. Williams,et al.  Prolonged ambulatory canine colonic motility. , 1995, The American journal of physiology.

[20]  O Baffa,et al.  An AC biosusceptometer to study gastric emptying. , 1992, Medical physics.

[21]  S. Sarna Physiology and pathophysiology of colonic motor activity , 1991, Digestive Diseases and Sciences.

[22]  S. Sarna Physiology and pathophysiology of colonic motor activity , 1991, Digestive Diseases and Sciences.

[23]  S. Phillips,et al.  Measurement of tone in canine colon. , 1991, The American journal of physiology.

[24]  S. Sarna,et al.  Colonic motor response to a meal in dogs. , 1989, The American journal of physiology.

[25]  E. Daniel,et al.  Pacemaker activity recorded in interstitial cells of Cajal of the gastrointestinal tract. , 1989, The American journal of physiology.

[26]  F. Azpiroz,et al.  Cyclic motility in canine colon: Responses to feeding and perfusion , 1989, Digestive Diseases and Sciences.

[27]  K. Sanders,et al.  Interaction of two electrical pacemakers in muscularis of canine proximal colon. , 1987, The American journal of physiology.

[28]  J. Huizinga Myoelectric correlates of colonic motor complexes and contractile activity. , 1986, The American journal of physiology.

[29]  E. Daniel,et al.  Control of human colonic motor function , 1986, Digestive Diseases and Sciences.

[30]  S. Sarna Myoelectric correlates of colonic motor complexes and contractile activity. , 1986, American Journal of Physiology.

[31]  W. Chey,et al.  Study of electromechanical activity of the stomach in humans and in dogs with particular attention to tachygastria. , 1984, Gastroenterology.

[32]  T. Y. El-Sharkawy Electrical activities of the muscle layers of the canine colon. , 1983, The Journal of physiology.

[33]  L. Buéno,et al.  Colonic myoelectrical activity and propulsion in the dog , 1980, Digestive Diseases and Sciences.

[34]  E. Frei,et al.  Study of gastric emptying using a ferromagnetic tracer. , 1977, Gastroenterology.

[35]  L. Elveback,et al.  Patterns of canine gastric electrical activity. , 1969, The American journal of physiology.

[36]  J. Misiewicz,et al.  Comparison of the effect of meals and prostigmine on the proximal and distal colon in patients with and without diarrhoea. , 1966, Gut.

[37]  L. Ds,et al.  Colonic myoelectrical activity and propulsion in the dog , 2005, Digestive Diseases and Sciences.

[38]  S. Sarna Physiology and pathophysiology of colonic motor activity (1). , 1991, Digestive diseases and sciences.

[39]  S N Reddy,et al.  Frequency analysis of gut EMG. , 1987, Critical reviews in biomedical engineering.