Highly sensitive and selective electrochemical sensor based on high-quality graphene/nafion nanocomposite for voltammetric determination of nebivolol

Abstract This research is concerned with the development of a facile, highly selective and sensitive graphene-based electrochemical nanosensing material for the detection of nebivolol (NBV). It is the first report that the electrochemical behaviour and quantitative analysis of NBV were investigated in detail on high-quality graphene/nafion nanocomposite modified electrode (GRE/NFN) using voltammetric techniques. The structure of high-quality graphene (GR) was characterized by Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA) and transmission electron microscopy (TEM). A broad peak at 1025 mV and well-defined peak at 1325 mV were observed by adsorptive stripping differential pulse voltammetry (AdsDPV) on GRE/NFN in the detection of NBV. Considering that the peak appeared at 1325 mV, GRE/NFN showed that NBV had a high electrocatalytic performance towards the NBV in the linear range from 0.5 to 24.0 μM with a low detection limit of 46 nM under the optimal experimental conditions. The GRE/NFN could be a promising material with high sensitivity and selectivity for the electrochemical sensing of NBV and can also be adopted to determine NBV in pharmaceutical and clinical samples.

[1]  R. Ruoff,et al.  Chemical methods for the production of graphenes. , 2009, Nature nanotechnology.

[2]  E. Laviron,et al.  Adsorption, autoinhibition and autocatalysis in polarography and in linear potential sweep voltammetry , 1974 .

[3]  Allen J. Bard,et al.  Electrochemical Methods: Fundamentals and Applications , 1980 .

[4]  Hejun Li,et al.  Graphene nanosheets synthesis via chemical reduction of graphene oxide using sodium acetate trihydrate solution , 2014 .

[5]  T. Rao,et al.  Electrochemically synthesized partially reduced graphene oxide modified glassy carbon electrode for individual and simultaneous voltammetric determination of ascorbic acid, dopamine and uric acid , 2014 .

[6]  Alka Gupta,et al.  SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF NEBIVOLOL HYDROCHLORIDE AND AMLODIPINE BESYLATE IN TABLETS , 2009 .

[7]  T. Pal,et al.  Simultaneous determination of fixed dose combination of nebivolol and valsartan in human plasma by liquid chromatographic-tandem mass spectrometry and its application to pharmacokinetic study. , 2007, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[8]  Li Hua,et al.  Glucose sensor based on an electrochemical reduced graphene oxide-poly(L-lysine) composite film modified GC electrode. , 2012, The Analyst.

[9]  T. Pham,et al.  One-step reduction of graphene oxide with l-glutathione , 2011 .

[10]  Yongling Du,et al.  Sensitive determination of vanillin based on an arginine functionalized graphene film , 2014 .

[11]  P. He,et al.  Simultaneous electrochemical determination of ascorbic acid, dopamine and uric acid using a palladium nanoparticle/graphene/chitosan modified electrode , 2013 .

[12]  A. Önal.  Spectrophotometric and spectrofluorimetric determination of some drugs containing secondary amino group in bulk drug and dosage forms via derivatization with 7-Chloro-4-Nitrobenzofurazon , 2011 .

[13]  Shenhao Chen,et al.  Highly sensitive and selective detection of dopamine based on hollow gold nanoparticles-graphene nanocomposite modified electrode. , 2013, Colloids and surfaces. B, Biointerfaces.

[14]  Yuyan Shao,et al.  Graphene Based Electrochemical Sensors and Biosensors: A Review , 2010 .

[15]  B. Rezaei,et al.  Adsorptive stripping voltammetry determination of methyldopa on the surface of a carboxylated multiwall carbon nanotubes modified glassy carbon electrode in biological and pharmaceutical samples. , 2013, Colloids and surfaces. B, Biointerfaces.

[16]  Y. Yoon,et al.  High-Quality Reduced Graphene Oxide by a Dual-Function Chemical Reduction and Healing Process , 2013, Scientific Reports.

[17]  Yuzhong Zhang,et al.  A sensitive electrochemical DNA biosensor based on gold nanomaterial and graphene amplified signal , 2014 .

[18]  Yuehe Lin,et al.  Synthesis of graphene nanosheets via oxalic acid-induced chemical reduction of exfoliated graphite oxide , 2012 .

[19]  Junhong Zhao,et al.  A novel nonenzymatic H2O2 sensor based on cobalt hexacyanoferrate nanoparticles and graphene composite modified electrode , 2015 .

[20]  C. Parthiban,et al.  SIMULTANEOUS ESTIMATION AND VALIDATION OF NEBIVOLOL AND VALSARTAN IN TABLET DOSAGE FORM BY RP-HPLC , 2014 .

[21]  A. Srivastava,et al.  Adsorptive stripping differential pulse voltammetric determination of metoprolol at Nafion-CNT-nano-composite film sensor , 2013 .

[22]  Alessandra Bonanni,et al.  Graphene for electrochemical sensing and biosensing , 2010 .

[23]  H. Neels,et al.  Optimization and validation of a hydrophilic interaction liquid chromatography–tandem mass spectrometry method for the determination of 13 top-prescribed pharmaceuticals in influent wastewater , 2010, Analytical and bioanalytical chemistry.

[24]  Ashwini K. Srivastava,et al.  Adsorptive stripping differential pulse voltammetric determination of venlafaxine and desvenlafaxine employing Nafion–carbon nanotube composite glassy carbon electrode , 2011 .

[25]  S. Nuttall,et al.  A comparison of the β1‐selectivity of three β1‐selective β‐blockers , 2003 .

[26]  Hüseyin Çelikkan,et al.  A new generation electrochemical sensor based on Graphene nanosheets/Gold nanoparticles/Nafion nanocomposite for determination of Silodosin , 2015 .

[27]  F. Rossi,et al.  Pharmacology of nebivolol. , 1998, Pharmacological research.

[28]  V. Lanchote,et al.  Stereoselective analysis of nebivolol isomers in human plasma by high-performance liquid chromatography-tandem mass spectrometry: application in pharmacokinetics. , 2013, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[29]  Engin Er,et al.  An efficient way to reduce graphene oxide by water elimination using phosphoric acid , 2014 .

[30]  Andre K. Geim,et al.  The rise of graphene. , 2007, Nature materials.

[31]  Zhaoxia Wang,et al.  Electrochemical detection of dopamine in the presence of epinephrine, uric acid and ascorbic acid using a graphene-modified electrode , 2012 .

[32]  S. Rajput,et al.  Quantitative determination of nebivolol from human plasma using liquid chromatography-tandem mass spectrometry. , 2013, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[33]  Qin Xu,et al.  Graphene–Au nanoparticles nanocomposite film for selective electrochemical determination of dopamine , 2012 .