Single HeLa and MCF-7 cell measurement using minimized impedance spectroscopy and microfluidic device.

This study presents an impedance measurement system for single-cell capture and measurement. The microwell structure which utilizes nDEP force is used to single-cell capture and a minimized impedance spectroscopy which includes a power supply chip, an impedance measurement chip and a USB microcontroller chip is used to single-cell impedance measurement. To improve the measurement accuracy of the proposed system, Biquadratic fitting is used in this study. The measurement accuracy and reliability of the proposed system are compared to those of a conventional precision impedance analyzer. Moreover, a stable material, latex beads, is used to study the impedance measurement using the minimized impedance spectroscopy with cell-trapping device. Finally, the proposed system is used to measure the impedance of HeLa cells and MCF-7 cells. The impedance of single HeLa cells decreased from 9.55 × 10(3) to 3.36 × 10(3) Ω and the impedance of single MCF-7 cells decreased from 3.48 × 10(3) to 1.45 × 10(3) Ω at an operate voltage of 0.5 V when the excitation frequency was increased from 11 to 101 kHz. The results demonstrate that the proposed impedance measurement system successfully distinguishes HeLa cells and MCF-7 cells.

[1]  M. Saijo,et al.  Modification of endothelial cell functions by Hantaan virus infection: prolonged hyper-permeability induced by TNF-alpha of hantaan virus-infected endothelial cell monolayers , 2004, Archives of Virology.

[2]  Richard D. Rabbitt,et al.  Electric impedance spectroscopy using microchannels with integrated metal electrodes , 1999 .

[3]  Hywel Morgan,et al.  On-chip epithelial barrier function assays using electrical impedance spectroscopy. , 2010, Lab on a chip.

[4]  Timothy B. Stockwell,et al.  The Sequence of the Human Genome , 2001, Science.

[5]  T. Geng,et al.  Microfluidic Biochip for Impedance Spectroscopy of Biological Species , 2001 .

[6]  Chuanmin Ruan,et al.  Detection of viable Salmonella typhimurium by impedance measurement of electrode capacitance and medium resistance. , 2003, Biosensors & bioelectronics.

[7]  Joachim Wegener,et al.  Bioelectrical impedance assay to monitor changes in cell shape during apoptosis. , 2004, Biosensors & bioelectronics.

[8]  J. V. Moran,et al.  Initial sequencing and analysis of the human genome. , 2001, Nature.

[9]  N. Verma,et al.  A disposable microbial based biosensor for quality control in milk. , 2003, Biosensors & bioelectronics.

[10]  B. Dunn,et al.  A MEMS based amperometric detector for E. coli bacteria using self-assembled monolayers. , 2001, Biosensors & bioelectronics.

[11]  S. Pecorelli,et al.  Cancer in Women , 1963 .

[12]  Koji Asami,et al.  Simulation for the dielectric images of single biological cells obtained using a scanning dielectric microscope , 2008 .

[13]  Jing Fang,et al.  Detection of bacterial cells by impedance spectra via fluidic electrodes in a microfluidic device. , 2010, Lab on a chip.

[14]  Ling-Sheng Jang,et al.  Microfluidic device for cell capture and impedance measurement , 2007, Biomedical microdevices.

[15]  V. Jordan,et al.  MCF-7: the first hormone-responsive breast cancer cell line. , 1997, Cancer research.

[16]  'Top Down' Approaches for the Study of Single-Cells: Micro-Engineering and Electrical Phenotype , 2006 .

[17]  R. Mansel,et al.  Biphasic effects of 17‐β‐estradiol on expression of occludin and transendothelial resistance and paracellular permeability in human vascular endothelial cells , 2003, Journal of cellular physiology.

[18]  Ling-Sheng Jang,et al.  Single-cell trapping utilizing negative dielectrophoretic quadrupole and microwell electrodes. , 2009, Biosensors & bioelectronics.

[19]  Kwang-Seok Yun,et al.  Single-cell manipulation on microfluidic chip by dielectrophoretic actuation and impedance detection , 2010 .

[20]  Damijan Miklavcic,et al.  Second-order model of membrane electric field induced by alternating external electric fields , 2000, IEEE Transactions on Biomedical Engineering.

[21]  Gwo-Bin Lee,et al.  Miniature RT–PCR system for diagnosis of RNA-based viruses , 2005, Nucleic acids research.

[22]  T. Yamamoto,et al.  MEMS-based biochip for the characterization of single red blood cell , 2005, 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology.

[23]  Liwei Lin,et al.  A single cell electrophysiological analysis device with embedded electrode , 2007 .

[24]  Richard D Rabbitt,et al.  Single cell electric impedance topography: mapping membrane capacitance. , 2009, Lab on a chip.

[25]  J. Judy,et al.  Design and fabrication of a micromachined planar patch-clamp substrate with integrated microfluidics for single-cell measurements , 2006, Journal of Microelectromechanical Systems.

[26]  Laurent Giovangrandi,et al.  Sensitivity of cell-based biosensors to environmental variables. , 2005, Biosensors & bioelectronics.