Production of poly(hydroxyalkanoic acid).

Poly(hydroxyalkanoic acid) [PHA] is accumulated by numerous microorganisms as an energy reserve material under unbalanced growth conditions in the presence of excess carbon source. In spite of being a good candidate for biodegradable thermoplastics, their high price compared with conventional plastics currently in use has limited their availability in a wide range of applications. With the aim of reducing the high production cost of PHA, much effort is currently being devoted to improve productivity by employing various microorganisms and by developing efficient culture techniques. Several processes recently developed and employed for the production of PHA by various bacteria are described.

[1]  R. M. Lafferty,et al.  Cloning and expression of the levanase gene in Alcaligenes eutrophus H16 enables the strain to hydrolyze sucrose , 1989 .

[2]  J. Kingma,et al.  Characterization of intracellular inclusions formed by Pseudomonas oleovorans during growth on octane , 1983, Journal of bacteriology.

[3]  A. Steinbüchel,et al.  Molecular analysis of the Alcaligenes eutrophus poly(3-hydroxybutyrate) biosynthetic operon: identification of the N terminus of poly(3-hydroxybutyrate) synthase and identification of the promoter , 1991, Journal of bacteriology.

[4]  G. W. Haywood,et al.  Accumulation of a Polyhydroxyalkanoate Containing Primarily 3-Hydroxydecanoate from Simple Carbohydrate Substrates by Pseudomonas sp. Strain NCIMB 40135 , 1990, Applied and environmental microbiology.

[5]  G. Huisman,et al.  Synthesis of poly-3-hydroxyalkanoates is a common feature of fluorescent pseudomonads , 1989, Applied and environmental microbiology.

[6]  S. Nakamura,et al.  Production of Biodegradable Copolyesters by Alcaligenes eutrophus , 1990 .

[7]  P. de Waard,et al.  13C nuclear magnetic resonance studies of Pseudomonas putida fatty acid metabolic routes involved in poly(3-hydroxyalkanoate) synthesis , 1994, Journal of bacteriology.

[8]  A. Sinskey,et al.  Poly-beta-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC). , 1989, The Journal of biological chemistry.

[9]  Takeshi Kobayashi,et al.  Enhanced β‐galactosidase production by high cell‐density culture of recombinant Bacillus subtilis with glucose concentration control , 1992, Biotechnology and bioengineering.

[10]  G. Gottschalk,et al.  Formation and Utilization of Poly-β-Hydroxybutyric Acid by Knallgas Bacteria (Hydrogenomonas) , 1961, Nature.

[11]  H. Chang,et al.  Production of Bacillus thuringiensis spores in total cell retention culture and two‐stage continuous culture using an internal ceramic filter system , 1993, Biotechnology and bioengineering.

[12]  F. Rodríguez-Valera,et al.  Effects of Culture Conditions on Poly(β-Hydroxybutyric Acid) Production by Haloferax mediterranei , 1990, Applied and environmental microbiology.

[13]  Y. Inoue,et al.  Structure and physical properties of bacterially synthesized polyesters , 1992 .

[14]  S. Lee,et al.  Comparison of recombinant Escherichia coli strains for synthesis and accumulation of poly‐(3‐hydroxybutyric acid) and morphological changes , 1994, Biotechnology and bioengineering.

[15]  H. Chang,et al.  Construction of plasmids, estimation of plasmid stability, and use of stable plasmids for the production of poly(3-hydroxybutyric acid) by recombinant Escherichia coli. , 1994, Journal of biotechnology.

[16]  J. Ramsay,et al.  Continuous Production of Long-Side-Chain Poly-β-Hydroxyalkanoates by Pseudomonas oleovorans , 1991, Applied and environmental microbiology.

[17]  S. Slater,et al.  Production of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) in a recombinant Escherichia coli strain , 1992, Applied and environmental microbiology.

[18]  T. Yamane.  Yield of poly‐D(‐)‐3‐hydroxybutyrate from various carbon sources: A theoretical study , 1993, Biotechnology and bioengineering.

[19]  T. Yamane,et al.  Synthesis of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) from Methanol and n-Amyl Alcohol by the Methylotrophic Bacteria Paracoccus denitrificans and Methylobacterium extorquens , 1992, Applied and environmental microbiology.

[20]  A. Ishizaki,et al.  Production of poly-β-hydroxybutyric acid from carbon dioxide by Alcaligenes eutrophus ATCC 17697T , 1991 .

[21]  E. Dawes,et al.  The regulation of poly-β-hydroxybutyrate metabolism in Azotobacter beijerinckii , 1973 .

[22]  G. Huisman,et al.  Pseudomonas putida KT2442 cultivated on glucose accumulates poly(3-hydroxyalkanoates) consisting of saturated and unsaturated monomers , 1992, Applied and environmental microbiology.

[23]  A. Shatzman.  Gene expression using gram-negative bacteria. , 1990, Current opinion in biotechnology.

[24]  H. Blanch,et al.  Recombinant Protein Expression in High Cell Density Fed-Batch Cultures of Escherichia Coli , 1992, Bio/Technology.

[25]  B. Witholt,et al.  Continuous production of poly(3-hydroxyalkanoates) by Pseudomonas oleovorans in a high-cell-density, two-liquid-phase chemostat , 1993 .

[26]  J. Lutkenhaus.  Regulation of cell division in E. coli. , 1990, Trends in genetics : TIG.

[27]  T. Yamane,et al.  n-Amyl alcohol as a substrate for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by bacteria , 1992 .

[28]  R. Gross,et al.  Plastics from bacteria and for bacteria: poly(beta-hydroxyalkanoates) as natural, biocompatible, and biodegradable polyesters. , 1990, Advances in biochemical engineering/biotechnology.

[29]  H. Chang,et al.  Production of poly(3-hydroxybutyric-co-3-hydroxyvaleric acid) by fed-batch culture of Alcaligenes eutrophus with substrate control using on-line glucose analyzer , 1994 .

[30]  Steven M. Schlasner,et al.  An automatic, on-line glucose analyzer for feed-back control of fed-batch growth of Escherichia coli , 1987 .

[31]  Stacy Fidler,et al.  Polyhydroxyalkanoate production in recombinant Escherichia coli. , 1992 .

[32]  K. Gerdes,et al.  The parB (hok/sok) Locus of Plasmid R1: A General Purpose Plasmid Stabilization System , 1988, Bio/Technology.

[33]  G. Huisman,et al.  Formation of Polyesters by Pseudomonas oleovorans: Effect of Substrates on Formation and Composition of Poly-(R)-3-Hydroxyalkanoates and Poly-(R)-3-Hydroxyalkenoates , 1988, Applied and environmental microbiology.

[34]  A. Steinbüchel,et al.  Cloning of the Alcaligenes eutrophus genes for synthesis of poly-beta-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coli , 1988, Journal of bacteriology.

[35]  R. Farris,et al.  The mechanical properties of a thermoplastic elastomer produced by the bacterium Pseudomonas oleovorans , 1992 .

[36]  P A Holmes,et al.  Applications of PHB - a microbially produced biodegradable thermoplastic , 1985 .

[37]  J. Ramsay,et al.  Effect of nitrogen limitation on long-side-chain poly-beta-hydroxyalkanoate synthesis by Pseudomonas resinovorans , 1992, Applied and environmental microbiology.

[38]  H. Chang,et al.  Membrane bioreactors: present and prospects. , 1991, Advances in biochemical engineering/biotechnology.

[39]  A. Anderson,et al.  Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. , 1990, Microbiological reviews.

[40]  T. Yamane.  Cultivation engineering of microbial bioplastics production , 1992 .

[41]  A. Ishizaki,et al.  Batch culture of Alcaligenes eutrophus ATCC 17697T using recycled gas closed circuit culture system , 1990 .

[42]  U. Pieper,et al.  Molecular basis for biosynthesis and accumulation of polyhydroxyalkanoic acids in bacteria. , 1992, FEMS microbiology reviews.

[43]  Cloning of poly(3-hydroxybutyric acid) synthase genes of Rhodobacter sphaeroides and Rhodospirillum rubrum and heterologous expression in Alcaligenes eutrophus. , 1992 .

[44]  R. Repaske,et al.  Dense autotrophic cultures of Alcaligenes eutrophus , 1976, Applied and environmental microbiology.

[45]  Tsuneo Yamane,et al.  Fed-batch techniques in microbial processes , 1984 .

[46]  B. Witholt,et al.  High cell density cultivation of Pseudomonas oleovorans: Growth and production of poly (3‐hydroxyalkanoates) in two‐liquid phase batch and fed‐batch systems , 1993, Biotechnology and bioengineering.

[47]  A. Steinbüchel,et al.  Formation of polyesters consisting of medium-chain-length 3-hydroxyalkanoic acids from gluconate by Pseudomonas aeruginosa and other fluorescent pseudomonads , 1990, Applied and environmental microbiology.

[48]  G. A. Ritchie,et al.  The non-involvement of cyl-carrir protein in poly-beta-hydroxybutyric acid biosynthesis in Azotobacter beijerinckii. , 1969, Biochemical Journal.

[49]  H. Chang,et al.  Effect of complex nitrogen source on the synthesis and accumulation of poly(3-hydroxybutyric acid) by recombinantEscherichia coli in flask and fed-batch cultures , 1994 .

[50]  D. W. Ribbons,et al.  Occurrence of Poly-β-Hydroxybutyrate in the Azotobacteriaceae , 1968 .

[51]  B. Janes,et al.  Molecular Characterization of the Poly-ß-Hydroxybutyrate Biosynthetic Pathway of Alcaligenes Eutrophus H16 , 1990 .

[52]  H. Zhang,et al.  Production of polyhydroxyalkanoates in sucrose-utilizing recombinant Escherichia coli and Klebsiella strains , 1994, Applied and environmental microbiology.

[53]  G W Luli,et al.  Comparison of growth, acetate production, and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations , 1990, Applied and environmental microbiology.

[54]  Edwin A. Dawes,et al.  Novel Microbial Polymers: An Introductory Overview , 1990 .

[55]  D. Riesenberg,et al.  High-cell-density cultivation of Escherichia coli. , 1991, Current opinion in biotechnology.

[56]  R. Gross,et al.  Pseudomonas oleovorans as a Source of Poly(β-Hydroxyalkanoates) for Potential Applications as Biodegradable Polyesters , 1988, Applied and environmental microbiology.

[57]  U. Hänggi.  Pilot scale production of PHB with Alcaligenes latus , 1990 .

[58]  H. Schwab,et al.  Stability of r-microbes: stabilization of plasmid vectors by the partitioning function of broad-host-range plasmid RP4. , 1993, Journal of biotechnology.

[59]  C. Chavarie,et al.  Biosynthesis of Poly-β-Hydroxyalkanoates from Pentoses by Pseudomonas pseudoflava , 1990, Applied and environmental microbiology.

[60]  J. Kingma,et al.  Physiology and polyester formation of Pseudomonas oleovorans in continuous two-liquid-phase cultures. , 1991 .

[61]  H. Chang,et al.  Production of Poly(β‐Hydroxybutyric Acid) by Recombinant Escherichia colia , 1994 .

[62]  H. Chang,et al.  Production of poly(3‐hydroxybutyric acid) by fed‐batch culture of Alcaligenes eutrophus with glucose concentration control , 1994, Biotechnology and bioengineering.

[63]  E. Dawes.  Novel biodegradable microbial polymers , 1990 .

[64]  W. Page,et al.  Growth of Azotobacter vinelandii UWD in Fish Peptone Medium and Simplified Extraction of Poly-β-Hydroxybutyrate , 1993, Applied and environmental microbiology.

[65]  Y. Doi,et al.  Biosynthesis of poly(3-hydroxy-alkanoates) in Pseudomonas aeruginosa AO-232 from 13C-labelled acetate and propionate. , 1993, International Journal of Biological Macromolecules.

[66]  W. Page,et al.  Hyperproduction of Poly-β-Hydroxybutyrate during Exponential Growth of Azotobacter vinelandii UWD , 1989, Applied and environmental microbiology.

[67]  Y. Doi,et al.  Cyclic nature of poly(3-hydroxyalkanoate) metabolism in Alcaligenes eutrophus , 1990 .

[68]  D. Byrom,et al.  Polymer synthesis by microorganisms: technology and economics , 1987 .

[69]  W. Page,et al.  Formation of poly(hydroxybutyrate-co-hydroxyvalerate) by Azotobacter vinelandii UWD , 1992, Applied and environmental microbiology.

[70]  F. Neidhardt,et al.  Physiology of the bacterial cell : a molecular approach , 1990 .

[71]  G. Huisman,et al.  Formation of polyester blends by a recombinant strain of Pseudomonas oleovorans: Different poly(3-hydroxyalkanoates) are stored in separate granules , 1993 .

[72]  C. Chavarie,et al.  Production of poly-(beta-hydroxybutyric-co-beta-hydroxyvaleric) acids , 1990, Applied and environmental microbiology.

[73]  Takahiro Suzuki,et al.  Control of carbon source supply and dissolved oxygen by use of carbon dioxide concentration of exhaust gas in fed-batch culture , 1986 .

[74]  A. Sinskey,et al.  Poly-beta-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Characterization of the genes encoding beta-ketothiolase and acetoacetyl-CoA reductase. , 1989, The Journal of biological chemistry.

[75]  Chang-ho Park,et al.  Production of Poly(hydroxybutyrate) Homopolymer and Copolymer from Ethanol and Propanol in a Fed‐Batch Culture , 1993 .