Geographical variation in prevalence of hepatitis B virus DNA in HBsAg negative patients.

AIMS--To study the geographical variation of the prevalence of hepatitis B virus (HBV) DNA in hepatitis B surface antigen (HBsAg) negative subjects. METHODS--A nested polymerase chain reaction (PCR) assay was used to amplify the core region of HBV. The assay was able to detect 10 molecules of a full length HBV plasmid. RESULTS--When applied to HBsAg negative paraffin wax embedded liver samples from Italy, Hong Kong, and the United Kingdom, a geographical variation in the prevalence of HBV-DNA positivity was noted. Two of 18 (11%) of Italian samples and 2/29 (6.9%) of Hong Kong samples were positive for HBV-DNA while none of the 70 cases from the United Kingdom was positive by nested PCR. Contamination by plasmid DNA was excluded using a novel method based on heteroduplex formation. One HBV-DNA positive case had idiopathic chronic active hepatitis, but the diagnoses in the other three HBV-DNA positive cases did not suggest any aetiological connection between HBV-DNA positivity and liver pathology. CONCLUSIONS--HBV-DNA could be detected in the liver tissues of a proportion of HBsAg negative subjects. The prevalence of such cases is related to the endemic rate of a geographical region. The use of HBV PCR on paraffin wax embedded tissues will be valuable for future studies on the molecular epidemiology of HBV.

[1]  K. Fleming,et al.  Heteroduplex formation as a means to exclude contamination in virus detection using PCR. , 1991, Nucleic Acids Research.

[2]  S. An,et al.  Removal of inhibitor(s) of the polymerase chain reaction from formalin fixed, paraffin wax embedded tissues. , 1991, Journal of clinical pathology.

[3]  Public Health Service inter-agency guidelines for screening donors of blood, plasma, organs, tissues, and semen for evidence of hepatitis B and hepatitis C. , 1991, MMWR. Recommendations and reports : Morbidity and mortality weekly report. Recommendations and reports.

[4]  O. Yokosuka,et al.  Detection and direct sequencing of hepatitis B virus genome by DNA amplification method. , 1991, Gastroenterology.

[5]  J. Wands,et al.  Characterization and biological properties of a hepatitis B virus isolated from a patient without hepatitis B virus serologic markers , 1990, Hepatology.

[6]  C. Bréchot.  Polymerase chain reaction. A new tool for the study of viral infections in hepatology. , 1990, Journal of hepatology.

[7]  P. Lampertico,et al.  Detection of hepatitis B virus DNA in formalin-fixed, paraffin-embedded liver tissue by the polymerase chain reaction. , 1990, The American journal of pathology.

[8]  R. Miller,et al.  Rapid and sensitive method for the detection of serum hepatitis B virus DNA using the polymerase chain reaction technique , 1989, Journal of clinical microbiology.

[9]  K. Fleming,et al.  In vitro amplification of hepatitis B virus sequences from liver tumour DNA and from paraffin wax embedded tissues using the polymerase chain reaction. , 1989, Journal of clinical pathology.

[10]  S. Kwok,et al.  Avoiding false positives with PCR , 1989, Nature.

[11]  H. Thomas,et al.  Antenatal screening for hepatitis B is medically and economically effective in the prevention of vertical transmission: three years experience in a London hospital. , 1989, The Quarterly journal of medicine.

[12]  R. Purcell,et al.  Detection of serum hepatitis B virus DNA in patients with chronic hepatitis using the polymerase chain reaction assay. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[13]  C. Bréchot,et al.  TRANSMISSION OF HEPATITIS B FROM HEPATITIS-B-SERONEGATIVE SUBJECTS , 1988, The Lancet.

[14]  K. A. Fleming,et al.  FALSE-POSITIVE RESULTS AND THE POLYMERASE CHAIN REACTION , 1988, The Lancet.

[15]  P. Pasquini,et al.  HBsAg carriers among pregnant women in Italy: results from the screening during a vaccination campaign against hepatitis B. , 1988, Public health.

[16]  K. Mullis,et al.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. , 1988, Science.

[17]  B. Hansson,et al.  Lack of hepatitis B virus DNA sequences in sera from patients with acute and chronic liver diseases diagnosed as non-A, non-B-hepatitis. , 2008, Liver.

[18]  O. Yokosuka,et al.  Hepatitis B virus RNA transcripts and DNA in chronic liver disease. , 1986, The New England journal of medicine.

[19]  K. Mullis,et al.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. , 1985, Science.

[20]  J. Wands,et al.  Hepatitis B virus DNA in patients with chronic liver disease and negative tests for hepatitis B surface antigen. , 1985, The New England journal of medicine.

[21]  H. Blum,et al.  Hepatitis B Viral Nucleotide Sequences in Non‐A, Non‐B or Hepatitis B Virus‐Related Chronic Liver Disease , 1984, Hepatology.

[22]  PREVENTION OF PRIMARY LIVER CANCER: Report on a Meeting of a W.H.O. Scientific Group , 1983, The Lancet.

[23]  A. Zuckerman,et al.  Failure to detect nucleic acid homology between some non‐A, Non‐B viruses and hepatitis B virus DNA , 1983, Journal of medical virology.

[24]  N. Gough,et al.  Expression of the hepatitis B virus surface, core and E antigen genes by stable rat and mouse cell lines. , 1982, Journal of molecular biology.