Chemoprevention with Aqueous Extract of Butea monosperma flowers results in normalization of nuclear morphometry and inhibition of a proliferation marker in liver tumors

Butea monosperma (Lam.) (family: Fabaceae) popularly known as ‘Palas’ or ‘fire of forest’ has been used traditionally as a hepatoprotective agent. This study evaluated the hepatoprotective and antitumorigenic properties of the aqueous extract and butanol fractions of B. monosperma flowers in animal models. Dried flowers of B. monosperma were extracted with water and fractionated further using n‐butanol. The hepatoprotective activity of the aqueous extract was initially confirmed in a carbon tetrachloride‐induced liver damage model of rats. Oral administration of the aqueous extract produced a strong hepatoprotective effect similar to silymarin and normalized the serum levels of ALT, AST, bilirubin and triglyceride in rats. However, it did not affect the levels of glutathione and malondialdehyde which are oxidative stress markers in liver. Intraperitoneal administration of the aqueous extract in the X15‐myc oncomice not only maintained liver architecture and nuclear morphometry but also down‐regulated the serum VEGF levels. Immunohistochemical staining of liver sections with anti‐Ribosomal protein S27a antibody showed post‐treatment abolition of this proliferation marker from the tumor tissue. The butanol fractions, however, did not show antitumorigenic activity. Thus, the aqueous extract of B. monosperma flowers is not only hepatoprotective but also antitumorigenic by preserving the nuclear morphometry of the liver. Copyright © 2010 John Wiley & Sons, Ltd.

[1]  Vijay Kumar,et al.  Chemopreventive and anti-cancer properties of the aqueous extract of flowers of Butea monosperma. , 2010, Journal of ethnopharmacology.

[2]  R. Poon,et al.  Vascular Changes in Hepatocellular Carcinoma , 2008, Anatomical record.

[3]  A. Khade,et al.  A Comprehensive Review on Butea monosperma (Lam.) Kuntze , 2007 .

[4]  S. Fan,et al.  High Serum Vascular Endothelial Growth Factor Levels Predict Poor Prognosis after Radiofrequency Ablation of Hepatocellular Carcinoma: Importance of Tumor Biomarker in Ablative Therapies , 2007, Annals of Surgical Oncology.

[5]  Vijay L Kumar,et al.  Anticancer and cytotoxic properties of the latex of Calotropis procera in a transgenic mouse model of hepatocellular carcinoma. , 2006, World journal of gastroenterology.

[6]  M. Doble,et al.  Potential synergism of natural products in the treatment of cancer , 2006, Phytotherapy research : PTR.

[7]  Peter Carmeliet,et al.  VEGF as a Key Mediator of Angiogenesis in Cancer , 2005, Oncology.

[8]  J. Sung,et al.  Cyclooxygenase-2 pathway correlates with vascular endothelial growth factor expression and tumor angiogenesis in hepatitis B virus-associated hepatocellular carcinoma. , 2004, International journal of oncology.

[9]  Vijay Kumar,et al.  Transgenic mouse models of hepatitis B virus‐associated hepatocellular carcinoma , 2003, Reviews in medical virology.

[10]  Vijay Kumar,et al.  Hepatocellular carcinoma in a hepatitis B ‘x’ transgenic mouse model: A sequential pathological evaluation , 2003, Journal of gastroenterology and hepatology.

[11]  A. Goldhirsch,et al.  Low-dose oral methotrexate and cyclophosphamide in metastatic breast cancer: antitumor activity and correlation with vascular endothelial growth factor levels. , 2002, Annals of oncology : official journal of the European Society for Medical Oncology.

[12]  M. Yamamoto,et al.  Enhanced expression of S8, L12, L23a, L27 and L30 ribosomal protein mRNAs in human hepatocellular carcinoma. , 2001, Anticancer research.

[13]  A. Ryo,et al.  Enhanced expression of translation factor mRNAs in hepatocellular carcinoma. , 2000, Anticancer research.

[14]  P. Pushpangadan,et al.  DEVELOPMENT OF PHYTOMEDICINES FOR LIVER DISEASES , 1999 .

[15]  M. Ikeguchi,et al.  Computerized nuclear morphometry of hepatocellular carcinoma and its relation to proliferative activity , 1998, Journal of surgical oncology.

[16]  S. Ye,et al.  Significance of vascular endothelial growth factor mRNA expression in invasion and metastasis of hepatocellular carcinoma. , 1998, Journal of experimental & clinical cancer research : CR.

[17]  H. Wagner,et al.  Isoputrin and Butrin, the Antihepatotoxic Principles of Butea monosperma Flowers1 , 1986 .

[18]  M. Tohkin,et al.  Assay Method for Antihepatotoxic Activity Using Carbon Tetrachloride Induced Cytotoxicity in Primary Cultured Hepatocytes* , 1983, Planta medica.

[19]  P. O'gorman,et al.  Serum enzyme levels in alcoholism and drug dependency. , 1975, Journal of clinical pathology.

[20]  T. R. Seshadri,et al.  The glucosides of Butea monosperma , 1970 .

[21]  P. Boll,et al.  ON THE STRUCTURE OF NEOHESPERIDIN , 1964 .

[22]  G. Ellman,et al.  Tissue sulfhydryl groups. , 1959, Archives of biochemistry and biophysics.

[23]  T. R. Seshadri,et al.  The glycosidic components of the flowers ofButea frondosa , 1941 .

[24]  S. Sultana,et al.  Chemoprevention by Butea monosperma of hepatic carcinogenesis and oxidative damage in male wistar rats. , 2006, Asian Pacific journal of cancer prevention : APJCP.

[25]  H. Wagner,et al.  Isobutrin and butrin, the antihepatotoxic principles of Butea monosperma flowers. , 1986, Planta medica.

[26]  S. Aust,et al.  Microsomal lipid peroxidation. , 1978, Methods in enzymology.