Characterization of a novel gram-positive, catalase-negative coccus from horses: description of Eremococcus coleocola gen. nov., sp. nov.

Two strains of an unknown Gram-positive, catalase-negative, facultatively anaerobic coccus originating from the reproductive tract of horses were characterized by phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies demonstrated that the two strains constitute a new subline within the lactic-acid group of bacteria, close to, but distinct from, Abiotrophia defectiva, Globicatella sanguinis and close relatives. The unknown bacterium was readily distinguished from other described Gram-positive, catalase-negative cocci by biochemical tests and electrophoretic analysis of whole-cell proteins. On the basis of phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as Eremococcus coleocola gen. nov., sp. nov. The type strain of Eremococcus coleocola is CCUG 38207T.

[1]  P. Lawson,et al.  Ignavigranum ruoffiae sp. nov., isolated from human clinical specimens. , 1999, International journal of systematic bacteriology.

[2]  P. Lawson,et al.  Facklamia ignava sp. nov., Isolated from Human Clinical Specimens , 1998, Journal of Clinical Microbiology.

[3]  P. Lawson,et al.  Phenotypic and phylogenetic characterization of some Globicatella-like organisms from human sources: description of Facklamia hominis gen. nov., sp. nov. , 1997, International journal of systematic bacteriology.

[4]  M. Collins,et al.  Proposal to reclassify Leuconostoc oenos as Oenococcus oeni [corrig.] gen. nov., comb. nov.. , 1995, International journal of systematic bacteriology.

[5]  P. Vandamme,et al.  Analysis of electrophoretic whole organism protein fingerprints , 1994 .

[6]  M. Collins,et al.  Phenotypic and phylogenetic characterization of some Gemella-like organisms from human infections: description of Dolosigranulum pigrum gen. nov., sp. nov. , 1993, The Journal of applied bacteriology.

[7]  M. Collins,et al.  Taxonomic studies on some leuconostoc-like organisms from fermented sausages: description of a new genus Weissella for the Leuconostoc paramesenteroides group of species. , 1993, The Journal of applied bacteriology.

[8]  M. Collins,et al.  Globicatella sanguis gen.nov., sp.nov., a new gram-positive catalase-negative bacterium from human sources. , 1992, The Journal of applied bacteriology.

[9]  M. Collins,et al.  Phylogenetic analysis of some Aerococcus-like organisms from urinary tract infections: description of Aerococcus urinae sp. nov. , 1992, Journal of general microbiology.

[10]  M. Collins,et al.  Phylogenetic analysis of Alloiococcus otitis gen. nov., sp. nov., an organism from human middle ear fluid. , 1992, International journal of systematic bacteriology.

[11]  M. Collins,et al.  The phylogeny of Aerococcus and Pediococcus as determined by 16S rRNA sequence analysis: description of Tetragenococcus gen. nov. , 1990, FEMS microbiology letters.

[12]  K. Schleifer,et al.  Transfer of Streptococcus lactis and related streptococci to the genus Lactococcus gen. nov. , 1985 .

[13]  J. Devereux,et al.  A comprehensive set of sequence analysis programs for the VAX , 1984, Nucleic Acids Res..

[14]  K. Schleifer,et al.  Transfer of Streptococcus faecalis and Streptococcus faecium to the Genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov. , 1984 .

[15]  E. I. Garvie.  Streptococcus raffinolactis Orla-Jensen and Hansen, a Group N Streptococcus Found in Raw Milk , 1978 .

[16]  K. Schleifer,et al.  Peptidoglycan Types of Bacterial Cell Walls and Their Taxonomic Implications , 1973, Bacteriological reviews.