Real-time and long-time in vivo imaging in the shortwave infrared window of perforator vessels for more precise evaluation of flap perfusion.

Effective real-time and long-time in vivo imaging for flap perfusion requires bright and stable imaging agents whose emissions can effectively penetrate live tissues without photobleaching. Compared to the standard imaging agent today - intraoperative indocyanine green (ICG), quantum dots (QDs) is a more attractive alternative due to its excellent optical properties including broad emission spectrum and stability against photobleaching. Recent studies have confirmed that the shortwave infrared window (SWIR) between 1000 and 2300 nm is the most sensitive spectral range for in vivo imaging due to its extremely low tissue absorption and autofluorescence. Here, we, for the first time, report a novel approach of flap perfusion assessment that provides real-time and long-time in vivo imaging using lead sulfide (PbS) QDs. Our results show that PbS QDs, as an imaging agent, can improve the stability of in vivo high-resolution images in a sustained manner, thus facilitating the precise evaluation of flap perfusion. In summary, compared to current imaging reporters, SWIR QDs have high photostability and deep tissue penetration, which makes them as promising in vivo imaging agents for more precise evaluation of flap perfusion.

[1]  A. Zunger,et al.  The Excitonic Exchange Splitting and Radiative Lifetime in PbSe Quantum Dots , 2007 .

[2]  R. Holzwarth,et al.  Mid-infrared optical frequency combs at 2.5 μm based on crystalline microresonators , 2013, Nature Communications.

[3]  Shuo Diao,et al.  Through-skull fluorescence imaging of the brain in a new near-infrared window , 2014, Nature Photonics.

[4]  N. Hildebrandt,et al.  Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors. , 2015, Chemical Society reviews.

[5]  Brad A. Kairdolf,et al.  Semiconductor quantum dots for bioimaging and biodiagnostic applications. , 2013, Annual review of analytical chemistry.

[6]  Shuo Diao,et al.  Fluorescence Imaging In Vivo at Wavelengths beyond 1500 nm. , 2015, Angewandte Chemie.

[7]  D. Pang,et al.  Ag₂Se quantum dots with tunable emission in the second near-infrared window. , 2013, ACS applied materials & interfaces.

[8]  Kevin Welsher,et al.  Deep-tissue anatomical imaging of mice using carbon nanotube fluorophores in the second near-infrared window , 2011, Proceedings of the National Academy of Sciences.

[9]  Dongmin Wu,et al.  Real-time in vivo visualization of tumor therapy by a near-infrared-II Ag2S quantum dot-based theranostic nanoplatform , 2015, Nano Research.

[10]  B. Wall,et al.  Rare-earth-doped biological composites as in vivo shortwave infrared reporters , 2013, Nature Communications.

[11]  T. Sakata,et al.  Aqueous synthesis of glutathione-coated PbS quantum dots with tunable emission for non-invasive fluorescence imaging in the second near-infrared biological window (1000-1400 nm). , 2013, Chemical communications.

[12]  J. Dimmock,et al.  Band Edge Structure of PbS, PbSe, and PbTe , 1964 .

[13]  Wai Yan Lam,et al.  Quantum dots for quantitative imaging: from single molecules to tissue , 2015, Cell and Tissue Research.

[14]  R. Fairhurst,et al.  Characterization of artificially generated PbS aerosols and their use within a respiratory bioaccessibility test. , 2010, The Analyst.

[15]  Dejian Zhou,et al.  Near-infrared fluorescent ribonuclease-A-encapsulated gold nanoclusters: preparation, characterization, cancer targeting and imaging. , 2013, Nanoscale.

[16]  Ravindran Girija Aswathy,et al.  Near-infrared quantum dots for deep tissue imaging , 2010, Analytical and bioanalytical chemistry.

[17]  Y. Wo,et al.  Recycled Synthesis of Whey-Protein-Capped Lead Sulfide Quantum Dots as the Second Near-Infrared Reporter for Bioimaging Application , 2016 .

[18]  R. Nitschke,et al.  Quantum dots versus organic dyes as fluorescent labels , 2008, Nature Methods.

[19]  F. Wise,et al.  Electronic structure and optical properties of PbS and PbSe quantum dots , 1997 .

[20]  J. G. Solé,et al.  1.3 μm emitting SrF2:Nd3+ nanoparticles for high contrast in vivo imaging in the second biological window , 2015, Nano Research.

[21]  M. C. Mancini,et al.  Bioimaging: second window for in vivo imaging. , 2009, Nature nanotechnology.

[22]  Jennifer A Hollingsworth,et al.  Pushing the band gap envelope: mid-infrared emitting colloidal PbSe quantum dots. , 2004, Journal of the American Chemical Society.

[23]  L Scott Levin,et al.  Early Experience with Fluorescent Angiography in Free-Tissue Transfer Reconstruction , 2009, Plastic and reconstructive surgery.

[24]  Miss A.O. Penney.  (b) , 1974, The New Yale Book of Quotations.

[25]  U. Rößler,et al.  Electronic Structure of PbS, PbSe, and PbTe , 1970 .

[26]  Yan Zhang,et al.  In vivo real-time visualization of mesenchymal stem cells tropism for cutaneous regeneration using NIR-II fluorescence imaging. , 2015, Biomaterials.

[27]  Dai-Wen Pang,et al.  Water-soluble Ag(2)S quantum dots for near-infrared fluorescence imaging in vivo. , 2012, Biomaterials.

[28]  Yan Zhang,et al.  In vivo real-time visualization of tissue blood flow and angiogenesis using Ag2S quantum dots in the NIR-II window. , 2014, Biomaterials.

[29]  A. Waggoner,et al.  Long-Term Retention of Fluorescent Quantum Dots In Vivo , 2008 .

[30]  M. Newman,et al.  An Investigation of the Application of Laser-Assisted Indocyanine Green Fluorescent Dye Angiography in Pedicle Transverse Rectus Abdominus Myocutaneous Breast Reconstruction , 2011, The Canadian journal of plastic surgery = Journal canadien de chirurgie plastique.

[31]  V. Bulović,et al.  1.3 μm to 1.55 μm Tunable Electroluminescence from PbSe Quantum Dots Embedded within an Organic Device , 2003 .

[32]  S. Gambhir,et al.  Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics , 2005, Science.

[33]  Yunxia Li,et al.  Highly Fluorescent Ribonuclease-A-Encapsulated Lead Sulfide Quantum Dots for Ultrasensitive Fluorescence in Vivo Imaging in the Second Near-Infrared Window , 2016, Chemistry of materials : a publication of the American Chemical Society.

[34]  C. Holm,et al.  Intraoperative evaluation of skin-flap viability using laser-induced fluorescence of indocyanine green. , 2002, British journal of plastic surgery.

[35]  P. Guyot-Sionnest,et al.  Interband and Intraband Optical Studies of PbSe Colloidal Quantum Dots , 2002 .

[36]  Dejian Zhou,et al.  Direct water-phase synthesis of lead sulfide quantum dots encapsulated by β-lactoglobulin for in vivo second near infrared window imaging with reduced toxicity. , 2016, Chemical communications.

[37]  Soojin Lim,et al.  NIR dyes for bioimaging applications. , 2010, Current opinion in chemical biology.

[38]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.