In vitro evaluation of anti-microbial spectrum of Acacia nilotica leaves and bark extracts against pathogens causing otitis infection

The antimicrobial activity of Acacia nilotica leaves and bark extracts were assayed against the six ear pathogens causing otitis infection namely, Staphylococcus aureus, Proteus mirabilis, Escherchia coli, Pseudomonas aeruginosa, Acinetobacter sp. and Candida albicans using agar well diffusion method. Organic leaves and bark extracts displayed antimicrobial activity against all the tested ear pathogens, while aqueous leaves extract showed activity against three tested bacterial ear pathogens. Aqueous bark extracts were unable to exhibit any antimicrobial activity. The highest antimicrobial activity of A. nilotica leaves and bark extracts were found against S. aureus with zone of inhibition of 23.6 mm and 20.6mm in acetonic extract with minimum inhibitory concentration value of 6.25mg/ml and 12.5 mg/ml. The MBC values ranged between 6.25mg/ml and 505mg/ml for the different bacterial ear pathogens while showed the common MFC value of 50 mg/ml against C. albicans. These results indicate that acetonic leaves extract of A. nilotica could be used as a source of antimicrobial agents to treat otitis infections.

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