Single-cell analysis of gene expression by fluorescence microscopy.

Gene regulation by two-component systems has traditionally been studied using assays that involve averages over large numbers of cells. Single-cell measurements of transcription offer a complementary approach that provides the distribution of gene expression among the population. This chapter focuses on methods for using fluorescence microscopy and fluorescent proteins to study gene expression in single cells.

[1]  Sierd Bron,et al.  Stripping Bacillus: ComK auto‐stimulation is responsible for the bistable response in competence development , 2005, Molecular microbiology.

[2]  François Taddei,et al.  Aging and Death in an Organism That Reproduces by Morphologically Symmetric Division , 2005, PLoS Biology.

[3]  E M Judd,et al.  Visualization of the movement of single histidine kinase molecules in live Caulobacter cells. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[4]  D. Dubnau,et al.  Bistability in the Bacillus subtilis K‐state (competence) system requires a positive feedback loop , 2005, Molecular microbiology.

[5]  Oscar P. Kuipers,et al.  Phenotypic variation in bacteria: the role of feedback regulation , 2006, Nature Reviews Microbiology.

[6]  Oscar P Kuipers,et al.  Phosphatases modulate the bistable sporulation gene expression pattern in Bacillus subtilis , 2005, Molecular microbiology.

[7]  Nathan C Shaner,et al.  A guide to choosing fluorescent proteins , 2005, Nature Methods.

[8]  Dylan T Burnette,et al.  Cytokinesis Monitoring during Development Rapid Pole-to-Pole Shuttling of a Signaling Protein by Localized Kinase and Phosphatase in Caulobacter , 2004, Cell.

[9]  E. Groisman,et al.  Making informed decisions: regulatory interactions between two-component systems. , 2003, Trends in microbiology.

[10]  R. Tsien,et al.  green fluorescent protein , 2020, Catalysis from A to Z.

[11]  H M Davey,et al.  Flow cytometric analysis of microorganisms. , 2000, Methods.

[12]  P. Maloney,et al.  Distribution of suboptimally induces -D-galactosidase in Escherichia coli. The enzyme content of individual cells. , 1973, Journal of molecular biology.

[13]  Konstantin A Lukyanov,et al.  Fluorescent proteins as a toolkit for in vivo imaging. , 2005, Trends in biotechnology.

[14]  Estíbaliz Larrainzar,et al.  Applications of autofluorescent proteins for in situ studies in microbial ecology. , 2005, Annual review of microbiology.

[15]  O. Kuipers,et al.  Visualization of Differential Gene Expression by Improved Cyan Fluorescent Protein and Yellow Fluorescent Protein Production in Bacillus subtilis , 2004, Applied and Environmental Microbiology.

[16]  P. Swain,et al.  Stochastic Gene Expression in a Single Cell , 2002, Science.

[17]  Gürol M. Süel,et al.  An excitable gene regulatory circuit induces transient cellular differentiation , 2006, Nature.

[18]  Ertugrul M. Ozbudak,et al.  Regulation of noise in the expression of a single gene , 2002, Nature Genetics.

[19]  W. Hillen,et al.  Quantitative analysis of gene expression with an improved green fluorescent protein. p6. , 2000, European journal of biochemistry.

[20]  L. Poulsen,et al.  New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria , 1998, Applied and Environmental Microbiology.

[21]  M. Goulian,et al.  Imaging OmpR localization in Escherichia coli , 2006, Molecular microbiology.

[22]  Mark Goulian,et al.  Continuous Control in Bacterial Regulatory Circuits , 2004, Journal of bacteriology.

[23]  Jeffrey H. Miller.  A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Rela , 1992 .

[24]  H M Shapiro,et al.  Microbial analysis at the single-cell level: tasks and techniques. , 2000, Journal of microbiological methods.

[25]  S. Leibler,et al.  An ultrasensitive bacterial motor revealed by monitoring signaling proteins in single cells. , 2000, Science.

[26]  X. Xie,et al.  Probing Gene Expression in Live Cells, One Protein Molecule at a Time , 2006, Science.

[27]  A. Szalay,et al.  Imaging of light emission from the expression of luciferases in living cells and organisms: a review. , 2002, Luminescence : the journal of biological and chemical luminescence.

[28]  G. Phillips,et al.  Green fluorescent protein--a bright idea for the study of bacterial protein localization. , 2001, FEMS microbiology letters.

[29]  N. Friedman,et al.  Stochastic protein expression in individual cells at the single molecule level , 2006, Nature.

[30]  Jay C D Hinton,et al.  Green fluorescent protein as a marker for conditional gene expression in bacterial cells. , 2002, Methods in enzymology.

[31]  H. Berg,et al.  Single-cell FRET imaging of phosphatase activity in the Escherichia coli chemotaxis system. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[32]  C. Southward,et al.  The dynamic microbe: green fluorescent protein brings bacteria to light , 2002, Molecular microbiology.

[33]  S. C. Winans,et al.  Unwounded plants elicit Agrobacterium vir gene induction and T‐DNA transfer: transformed plant cells produce opines yet are tumour free , 2005, Molecular microbiology.

[34]  J. Schmid,et al.  Evolutions in Science Triggered by Green Fluorescent Protein (GFP) , 2005, Chembiochem : a European journal of chemical biology.

[35]  J. Ferrell.  Self-perpetuating states in signal transduction: positive feedback, double-negative feedback and bistability. , 2002, Current opinion in cell biology.

[36]  M. Goulian,et al.  A simple system for converting lacZ to gfp reporter fusions in diverse bacteria. , 2006, Gene.