Determination of biogenic amines in Korean traditional fermented soybean paste (Doenjang).

Biogenic amines, produced by bacterial decarboxylation of amino acids, have been associated with toxicological symptoms in food products. Twenty-three samples of traditionally available Korean fermented soybean paste samples (Doenjang) were analyzed in order to determine the content of biogenic amines. Amines were extracted with 0.4M perchloric acid and derivatized with dansyl chloride. Nine biogenic amines were separated from Doenjang samples by high performance liquid chromatography using gradient elution (acetonitrile and ammonium acetate), and detected with spectrophotometric UV-vis detection at 254 nm. The pH value of all the samples was ranged from 4.8 to 6.0, and the strong amino acid decarboxylase activity was found to be in an acidic environment. The mean values of biogenic amines (tryptamine, 2-phenyl-ethylamine, putrescine, cadaverine, agmatine, histamine, tyramine, spermidine and spermine) determined in 23 Doenjang samples were found to be 18.37, 82.03, 70.84, 34.24, 47.32, 26.79, 126.66, 74.41 and 244.36 mg%, respectively. The findings of this study enhance the safety of not only Doenjang but other salted and/or fermented food products.

[1]  Steve L Taylor,et al.  Biogenic Amines in Cheese and other Fermented Foods: A Review. , 1991, Journal of food protection.

[2]  S. Oguri,et al.  Selective analysis of histamine in food by means of solid-phase extraction cleanup and chromatographic separation. , 2007, Journal of chromatography. A.

[3]  A. Tassoni,et al.  Free and conjugated polyamine content in Citrus sinensis Osbeck, cultivar Brasiliano N.L. 92, a Navel orange, at different maturation stages , 2004 .

[4]  L. J. Walker,et al.  Free and total putrescine in cerebrospinal fluid quantified by reversed-phase liquid chromatography. , 1984, Clinical Chemistry.

[5]  M. Nout.  Fermented foods and food safety. , 1994 .

[6]  Z. Loukou,et al.  Determination of biogenic amines as dansyl derivatives in alcoholic beverages by high-performance liquid chromatography with fluorimetric detection and characterization of the dansylated amines by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry. , 2003, Journal of chromatography. A.

[7]  D. Grierson,et al.  A histidine decarboxylase-like mRNA is involved in tomato fruit ripening , 1993, Plant Molecular Biology.

[8]  Jae‐Hyun Kim,et al.  Reduction of the biogenic amine contents in low salt-fermented soybean paste by gamma irradiation , 2005 .

[9]  M. Nout,et al.  EFFECT OF PROCESSING CONDITIONS ON THE FORMATION OF BIOGENIC AMINES AND ETHYL CARBAMATE IN SOYBEAN TEMPE , 1993 .

[10]  M. Boekel,et al.  Conditions allowing the formation of biogenic amines in cheese. 4. A. study of the kinetics of histamine formation in an infected Gouda cheese. , 1988 .

[11]  W. Holzapfel,et al.  Biogenic amines and their production by microorganisms in food , 1994 .

[12]  P. Koehler,et al.  Identification and Estimation of Histamine, Tryptamine, Phenethylamine and Tyramine in Soy Sauce by Thin‐Layer Chromatography of Dansyl Derivatives , 1983 .

[13]  Maurizio Quinto,et al.  An interference free amperometric biosensor for the detection of biogenic amines in food products. , 2007, Biosensors & bioelectronics.

[14]  H. Brückner,et al.  Liquid chromatographic determination of biogenic amines in fermented foods after derivatization with 3,5-dinitrobenzoyl chloride. , 2000, Journal of chromatography. A.

[15]  J. Barros-Velázquez,et al.  Changes in biogenic amines and microbiological analysis in albacore (Thunnus alalunga) muscle during frozen storage. , 1998, Journal of food protection.

[16]  P. Kalač,et al.  Levels of biogenic amines in typical vegetable products , 2002 .

[17]  P. Kalač,et al.  A review of dietary polyamines: Formation, implications for growth and health and occurrence in foods , 2005 .

[18]  A. Shalaby.  Significance of biogenic amines to food safety and human health , 1996 .

[19]  A. Shalaby.  Multidetection, semiquantitative method for determining biogenic amines in foods , 1995 .

[20]  A. Salleh,et al.  An amperometric biosensor for the rapid assessment of histamine level in tiger prawn (Penaeus monodon) spoilage , 2007 .

[21]  M. H. Silla Santos.  Biogenic amines: their importance in foods. , 1996, International journal of food microbiology.

[22]  H. Park,et al.  Irradiation effects on biogenic amines in Korean fermented soybean paste during fermentation , 2003 .

[23]  C. Lucy,et al.  Selective fluorometric detection of polyamines using micellar electrokinetic chromatography with laser-induced fluorescence detection. , 2002, Journal of chromatography. A.

[24]  C. Wittmann,et al.  Comparison of a capillary electrophoresis method with high-performance liquid chromatography for the determination of biogenic amines in various food samples. , 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[25]  R. Simard,et al.  Effect of Lactobacillus brevis, Saccharomyces uvarum and Grist Composition on Amine Formations in Beers , 1981 .

[26]  S. Bunčić,et al.  A study of factors influencing histamine production in meat , 1994 .

[27]  I. Fleet,et al.  Determination of biogenic diamines with a vaporisation derivatisation approach using solid-phase microextraction gas chromatography-mass spectrometry. , 2008, Food chemistry.

[28]  L. Conte,et al.  A survey on free biogenic amine content of fresh and preserved vegetables , 2005 .