Ralstonia pickettii-induced ataxia in immunodeficient mice.

We report here the characterization of an asymmetric ataxia syndrome (head tilt and circling, with death in the most severe cases) demonstrated by profoundly immunodeficient mice housed at the Institut Curie SPF facility. The immune system of the affected mice had been genetically modified so that they were deficient in both B and T cells. Extensive bacteriologic, parasitic, serologic, and histopathologic analysis of the affected animals and their healthy controls led us to identify Ralstonia pickettii as the causative agent of the ataxic syndrome. The outbreak was managed through a test-and-cull process. Even though they also carried Ralstonia pickettii, immunocompetent mice that were kept in the same facility, did not show any of the signs that were expressed by their immunodeficient counterparts. This case highlights the difficulty of maintaining immunocompetent and immunodeficient mice in the same microbiologic unit and the importance of enlarging the spectrum of health monitoring to opportunistic agents when investigating clinical cases in populations of immunocompromised rodents.

[1]  K. Pritchett-Corning,et al.  Contemporary prevalence of infectious agents in laboratory mice and rats , 2009, Laboratory animals.

[2]  J. Sundberg,et al.  Klebsiella oxytoca: opportunistic infections in laboratory rodents , 2008, Laboratory animals.

[3]  R. Stout Pathology of Laboratory Rodents and Rabbits, 3rd edition, D.H. Pearcy, S.W. Barthold. Blackwell Publishing (2007), 325 pages , 2008 .

[4]  D. Trune,et al.  Gram-negative pathogen Klebsiella oxytoca is associated with spontaneous chronic otitis media in Toll-like receptor 4-deficient C3H/HeJ mice , 2008, Acta oto-laryngologica.

[5]  J. B. Kempton,et al.  C3H/HeJ Mouse Model for Spontaneous Chronic Otitis Media , 2006, The Laryngoscope.

[6]  J. T. van der Logt,et al.  Prevalence of naturally occurring viral infections, Mycoplasma pulmonis and Clostridium piliforme in laboratory rodents in Western Europe screened from 2000 to 2003 , 2006, Laboratory animals.

[7]  M. P. Ryan,et al.  Ralstonia pickettii: a persistent gram-negative nosocomial infectious organism. , 2006, The Journal of hospital infection.

[8]  M. Biebl,et al.  Ralstonia pickettii-innocent bystander or a potential threat? , 2006, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[9]  E. Marques,et al.  Ralstonia pickettii and Burkholderia cepacia complex bloodstream infections related to infusion of contaminated water for injection. , 2005, The Journal of hospital infection.

[10]  Ronald N. Jones,et al.  Antimicrobial susceptibility of uncommonly isolated non-enteric Gram-negative bacilli. , 2005, International journal of antimicrobial agents.

[11]  S. Feldman,et al.  Outbreak of otitis media caused by Burkholderia gladioli infection in immunocompromised mice. , 2004, Comparative medicine.

[12]  P. Vandamme,et al.  Infection by Ralstonia Species in Cystic Fibrosis Patients: Identification of R. pickettii and R. mannitolilytica by Polymerase Chain Reaction , 2002, Emerging infectious diseases.

[13]  P. Rehbinder,et al.  Recommendations for the health monitoring of rodent and rabbit colonies in breeding and experimental units , 2002, Laboratory animals.

[14]  P. Beringer,et al.  Unusual respiratory bacterial flora in cystic fibrosis: microbiologic and clinical features , 2000, Current opinion in pulmonary medicine.

[15]  W. Trick,et al.  A multistate nosocomial outbreak of Ralstonia pickettii colonization associated with an intrinsically contaminated respiratory care solution. , 1999, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[16]  L. Pirofski,et al.  Mouse adenovirus type 1 causes a fatal hemorrhagic encephalomyelitis in adult C57BL/6 but not BALB/c mice , 1995, Journal of virology.

[17]  F. Rieux-Laucat,et al.  Defective human interleukin 2 receptor gamma chain in an atypical X chromosome-linked severe combined immunodeficiency with peripheral T cells. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[18]  M. Kotiw,et al.  Typing of strains from a single-source outbreak of Pseudomonas pickettii , 1993, Journal of clinical microbiology.

[19]  G. Eamens Bacterial and mycoplasmal flora of the middle ear of laboratory rats with otitis media. , 1984, Laboratory animal science.