A statistical model for calibration and computation of detection and quantification limits for low copy number environmental DNA samples

[1]  I. Salter,et al.  Environmental DNA concentrations are correlated with regional biomass of Atlantic cod in oceanic waters , 2019, Communications Biology.

[2]  V. Beneš,et al.  The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. , 2009, Clinical chemistry.

[3]  B. Sjögreen,et al.  Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR) , 2017, Biomolecular detection and quantification.

[4]  Eske Willerslev,et al.  Environmental DNA - An emerging tool in conservation for monitoring past and present biodiversity , 2015 .

[5]  C. Goldberg,et al.  Moving environmental DNA methods from concept to practice for monitoring aquatic macroorganisms , 2015 .

[6]  C. Richter,et al.  Reporting the limits of detection and quantification for environmental DNA assays , 2019, Environmental DNA.

[7]  Franklin A. Graybill,et al.  Theory and Application of the Linear Model , 1976 .

[8]  R. Brys,et al.  Influence of accuracy, repeatability and detection probability in the reliability of species-specific eDNA based approaches , 2019, Scientific Reports.

[9]  L. Waits,et al.  Critical considerations for the application of environmental DNA methods to detect aquatic species , 2016 .

[10]  D. Armbruster,et al.  Limit of blank, limit of detection and limit of quantitation. , 2008, The Clinical biochemist. Reviews.

[11]  F. Lyko,et al.  Early detection of an emerging invasive species: eDNA monitoring of a parthenogenetic crayfish in freshwater systems , 2019, Management of Biological Invasions.

[12]  L. Bernatchez,et al.  Pathway to Increase Standards and Competency of eDNA Surveys (PISCeS)—Advancing collaboration and standardization efforts in the field of eDNA , 2020 .

[13]  W. L. Chadderton,et al.  “Sight‐unseen” detection of rare aquatic species using environmental DNA , 2011 .

[14]  Robert S. Arkle,et al.  Estimating occupancy and abundance of stream amphibians using environmental DNA from filtered water samples , 2013 .

[15]  D. Fraser,et al.  Meta‐analysis supports further refinement of eDNA for monitoring aquatic species‐specific abundance in nature , 2019, Environmental DNA.

[16]  C. Helbing,et al.  Expansion of the known distribution of the coastal tailed frog, Ascaphus truei, in British Columbia, Canada, using robust eDNA detection methods , 2019, PloS one.

[17]  H. Doi,et al.  Environmental DNA analysis for estimating the abundance and biomass of stream fish , 2017 .

[18]  Franco Magno,et al.  A statistical overview on univariate calibration, inverse regression, and detection limits: Application to gas chromatography/mass spectrometry technique. , 2007, Mass spectrometry reviews.

[19]  H. Embke,et al.  Application of eDNA as a tool for assessing fish population abundance , 2020, Environmental DNA.

[20]  T. Rodgers,et al.  At the forefront: evidence of the applicability of using environmental DNA to quantify the abundance of fish populations in natural lentic waters with additional sampling considerations , 2017 .

[21]  Matteo Ricchi,et al.  A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything , 2017, Front. Microbiol..

[22]  Ramesh Ramakrishnan,et al.  Mathematical Analysis of Copy Number Variation in a DNA Sample Using Digital PCR on a Nanofluidic Device , 2008, PloS one.

[23]  S. Julious.  Two‐sided confidence intervals for the single proportion: comparison of seven methods by Robert G. Newcombe, Statistics in Medicine 1998; 17:857–872 , 2005, Statistics in medicine.

[24]  H. Doi,et al.  Use of Droplet Digital PCR for Estimation of Fish Abundance and Biomass in Environmental DNA Surveys , 2015, PloS one.

[25]  L. Bernatchez,et al.  Quantifying relative fish abundance with eDNA: a promising tool for fisheries management , 2016 .

[26]  Helen C. Rees,et al.  The application of eDNA for monitoring of the Great Crested Newt in the UK , 2014, Ecology and evolution.