Disseminated Emergomycosis in a Person with HIV Infection, Uganda

We describe emergomycosis in a patient in Uganda with HIV infection. We tested a formalin-fixed, paraffin-embedded skin biopsy to identify Emergomyces pasteurianus or a closely related pathogen by sequencing broad-range fungal PCR amplicons. Results suggest that emergomycosis is more widespread and genetically diverse than previously documented. PCR on tissue blocks may help clarify emergomycosis epidemiology.

[1]  S. Hartmann,et al.  Identification of Aspergillus and Mucorales in formalin-fixed, paraffin-embedded tissue samples: Comparison of specific and broad-range fungal qPCR assays , 2018, Medical mycology.

[2]  A. Stchigel,et al.  Phylogeny, ecology and taxonomy of systemic pathogens and their relatives in Ajellomycetaceae (Onygenales): Blastomyces, Emergomyces, Emmonsia, Emmonsiellopsis , 2018, Fungal Diversity.

[3]  N. Govender,et al.  Emergomyces: a New Genus of Dimorphic Fungal Pathogens Causing Disseminated Disease among Immunocompromised Persons Globally , 2018, Current Fungal Infection Reports.

[4]  J. Meis,et al.  Antifungal Susceptibility of Emerging Dimorphic Pathogens in the Family Ajellomycetaceae , 2017, Antimicrobial Agents and Chemotherapy.

[5]  D. Boutoille,et al.  Emmonsia pasteuriana : une cause rare d’infection fongique chez l’immunodéprimé , 2017 .

[6]  Christina A. Cuomo,et al.  Novel taxa of thermally dimorphic systemic pathogens in the Ajellomycetaceae (Onygenales) , 2017, Mycoses.

[7]  V. Rickerts.  Identification of fungal pathogens in Formalin-fixed, Paraffin-embedded tissue samples by molecular methods. , 2016, Fungal biology.

[8]  S. Rudramurthy,et al.  Disseminated Emmonsia pasteuriana infection in India: a case report and a review , 2016, Mycoses.

[9]  Benquan Wu,et al.  Disseminated infection caused by Emmonsia pasteuriana in a renal transplant recipient , 2015, The Journal of dermatology.

[10]  Hui Zhou,et al.  Cutaneous Disseminated Emmonsiosis Due to Emmonsia pasteuriana in a Patient With Cytomegalovirus Enteritis. , 2015, JAMA dermatology.

[11]  C. Cabellos,et al.  A second look at Emmonsia infection can make the difference , 2014, Transplant infectious disease : an official journal of the Transplantation Society.

[12]  D. Fredricks,et al.  Enhanced fungal DNA-extraction from formalin-fixed, paraffin-embedded tissue specimens by application of thermal energy. , 2012, Medical mycology.

[13]  D. Fredricks,et al.  Comparison of quantitative real time PCR with Sequencing and ribosomal RNA-FISH for the identification of fungi in Formalin fixed, paraffin-embedded tissue specimens , 2011, BMC infectious diseases.

[14]  C. Aznar,et al.  Detection of Histoplasma capsulatum DNA in human samples by real-time polymerase chain reaction. , 2010, Diagnostic microbiology and infectious disease.

[15]  D. Fredricks,et al.  Sequencing and Analysis of Fungal rRNA Operons for Development of Broad-Range Fungal PCR Assays , 2009, Applied and Environmental Microbiology.

[16]  E. Guého,et al.  Cutaneous disseminated mycosis in a patient with AIDS due to a new dimorphic fungus , 2008 .

[17]  K. Tamura,et al.  Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G+C-content biases. , 1992, Molecular biology and evolution.