Targeting the E1 replication protein to the papillomavirus origin of replication by complex formation with the E2 transactivator.

The mechanism by which transcription factors stimulate DNA replication in eukaryotes is unknown. Bovine papillomavirus DNA synthesis requires the products of the viral E1 gene and the transcriptional activator protein encoded by the E2 gene. Experimental data showed that the 68-kilodalton (kD) E1 protein formed a complex with the 48-kD E2 transcription factor. This complex bound specifically to the viral origin of replication, which contains multiple binding sites for E2. Repressor proteins encoded by the E2 open reading frame failed to complex with E1 suggesting that the 162-amino acid region of E2 that participates in transactivation contained critical determinants for interaction with E1. The physical association between a replication protein and a transcription factor suggests that transcriptional activator proteins may function in targeting replication initiator proteins to their respective origins of replication.

[1]  M. Botchan,et al.  Replication of bovine papillomavirus type 1 DNA initiates within an E2-responsive enhancer element , 1990, Journal of virology.

[2]  M. Botchan,et al.  Identification of a 68-kilodalton nuclear ATP-binding phosphoprotein encoded by bovine papillomavirus type 1 , 1990, Journal of virology.

[3]  E. Geiduschek,et al.  An RNA polymerase-binding protein that is required for communication between an enhancer and a promoter. , 1990, Science.

[4]  J. Settleman,et al.  Bovine papillomavirus E2 repressor mutant displays a high-copy-number phenotype and enhanced transforming activity , 1990, Journal of virology.

[5]  P. Howley,et al.  Phenotypic analysis of bovine papillomavirus type 1 E2 repressor mutants , 1990, Journal of virology.

[6]  P. Howley,et al.  Phosphorylation sites of the E2 transcriptional regulatory proteins of bovine papillomavirus type 1 , 1989, Journal of virology.

[7]  T. Kelly,et al.  Transcriptional activator nuclear factor I stimulates the replication of SV40 minichromosomes in vivo and in vitro , 1989, Cell.

[8]  B. Alberts,et al.  Enhancement of bacteriophage T4 late transcription by components of the T4 DNA replication apparatus. , 1989, Science.

[9]  E. A. O'neill,et al.  The proline-rich transcriptional activator of CTF/NF-I is distinct from the replication and DNA binding domain , 1989, Cell.

[10]  P. Howley,et al.  Genetic assignment of multiple E2 gene products in bovine papillomavirus-transformed cells , 1989, Journal of virology.

[11]  D. Lowy,et al.  Identification of bovine papillomavirus E1 mutants with increased transforming and transcriptional activity , 1989, Journal of virology.

[12]  M. Botchan,et al.  Specific recognition nucleotides and their DNA context determine the affinity of E2 protein for 17 binding sites in the BPV-1 genome. , 1989, Genes & development.

[13]  M. Botchan,et al.  Bovine papillomavirus type 1 encodes two forms of a transcriptional repressor: structural and functional analysis of new viral cDNAs , 1989, Journal of virology.

[14]  M. Challberg,et al.  Animal virus DNA replication. , 1989, Annual review of biochemistry.

[15]  D. Inlow,et al.  Large-Scale Insect Cell-Culture for Recombinant Protein Production , 1988, Bio/Technology.

[16]  P. Howley,et al.  Bovine papillomavirus type 1 E1 replication-defective mutants are altered in their transcriptional regulation , 1988, Journal of virology.

[17]  T. Baker,et al.  Transcriptional activation of initiation of replication from the E. coli chromosomal origin: An RNA-DNA hybrid near oriC , 1988, Cell.

[18]  J. Hurwitz,et al.  Localized melting and structural changes in the SV40 origin of replication induced by T‐antigen. , 1988, The EMBO journal.

[19]  E. A. O'neill,et al.  Transcription factor OTF-1 is functionally identical to the DNA replication factor NF-III. , 1988, Science.

[20]  D. Lowy,et al.  Bovine papilloma virus-transformed cells contain multiple E2 proteins. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[21]  M. Botchan,et al.  The product of the bovine papillomavirus type 1 modulator gene (M) is a phosphoprotein , 1988, Journal of virology.

[22]  A. Kornberg,et al.  Duplex opening by dnaA protein at novel sequences in initiation of replication at the origin of the E. coli chromosome , 1988, Cell.

[23]  M. DePamphilis.  Transcriptional elements as components of eukaryotic origins of DNA replication , 1988, Cell.

[24]  F. Blattner,et al.  Initiation protein induced helix destabilization at the λ origin: A prepriming step in DNA replication , 1988, Cell.

[25]  M. Summers,et al.  Trends in the Development of Baculovirus Expression Vectors , 1988, Bio/Technology.

[26]  K. Nasmyth,et al.  A yeast silencer contains sequences that can promote autonomous plasmid replication and transcriptional activation , 1987, Cell.

[27]  P. Howley,et al.  Mutational analysis of the 3' open reading frames and the splice junction at nucleotide 3225 of bovine papillomavirus type 1 , 1987, Journal of virology.

[28]  A. Kornberg,et al.  ATP activates dnaA protein in initiating replication of plasmids bearing the origin of the E. coli chromosome , 1987, Cell.

[29]  Stanley N Cohen,et al.  Bovine papilloma virus plasmids replicate randomly in mouse fibroblasts throughout S phase of the cell cycle , 1987, Cell.

[30]  P. Howley,et al.  Bovine papillomavirus transcriptional regulation: localization of the E2-responsive elements of the long control region , 1987, Journal of Virology.

[31]  F. Dean,et al.  Simian virus 40 (SV40) DNA replication: SV40 large T antigen unwinds DNA containing the SV40 origin of replication. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[32]  D. Lowy,et al.  Bovine papillomavirus E2 trans-activating gene product binds to specific sites in papillomavirus DNA , 1987, Nature.

[33]  D. Reisman,et al.  trans activation of an Epstein-Barr viral transcriptional enhancer by the Epstein-Barr viral nuclear antigen 1 , 1986, Molecular and cellular biology.

[34]  H. Echols,et al.  Multiple DNA-protein interactions governing high-precision DNA transactions. , 1986, Science.

[35]  Douglas W. Smith,et al.  The bacterial origin of replication, oriC , 1986, Cell.

[36]  D. E. Groff,et al.  Genetic analysis of the 3' early region transformation and replication functions of bovine papillomavirus type 1. , 1986, Virology.

[37]  D. DiMaio.  Nonsense mutation in open reading frame E2 of bovine papillomavirus DNA , 1986, Journal of virology.

[38]  P. Tegtmeyer,et al.  Functional interactions of the simian virus 40 core origin of replication with flanking regulatory sequences , 1986, Journal of virology.

[39]  P. Howley,et al.  Transactivation of a bovine papilloma virus transcriptional regulatory element by the E2 gene product , 1985, Cell.

[40]  D. Lane,et al.  An immunoaffinity purification procedure for SV40 large T antigen. , 1985, Virology.

[41]  M. Botchan,et al.  Genetic analysis of bovine papillomavirus type 1 trans-acting replication factors , 1985, Journal of virology.

[42]  W. Schaffner,et al.  Polyoma virus DNA replication requires an enhancer , 1984, Nature.

[43]  P. Howley,et al.  Localization and analysis of bovine papillomavirus type 1 transforming functions , 1984, Journal of virology.

[44]  I. Seif,et al.  A common function for polyoma virus large-T and papillomavirus E1 proteins? , 1984, Nature.

[45]  B. M. Alberts.  The DNA enzymology of protein machines. , 1984, Cold Spring Harbor symposia on quantitative biology.

[46]  M. Greaves,et al.  A one-step purification of membrane proteins using a high efficiency immunomatrix. , 1982, The Journal of biological chemistry.

[47]  R. Harland,et al.  Regulated replication of DNA microinjected into eggs of Xenopus laevis , 1980, Cell.