Rotation-translation device for condensed-phase spectroscopy with small sample volumes

We present and characterize an experimental device for optical spectroscopy with small sample volumes contained in a thin film. Employing rotational and translational motion, the sample transport speeds are high enough to offer a new sample volume for each interaction in time-resolved spectroscopy experiments working with a 1kHz repetition rate. This is especially suited for ultrafast femtosecond spectroscopy such as transient absorption spectroscopy or fluorescence upconversion. To reduce photodegradation and effects from local thermal heating, a large sample area is scanned in contrast to conventional devices with either only rotation or translation movements. For characterization of the setup, transient absorption experiments are carried out using the rotation-translation device and a conventional flow-cell setup, which exhibit similar signal-to-noise ratio in the two cases. The effects of photodegradation and diffusion are also investigated, demonstrating the suitability of the device for time-resolve...

[1]  E. Riedle,et al.  Switching dynamics of the photochromic 1,1-dicyano-2-(4-cyanophenyl)-1,8a-dihydroazulene probed by sub-30 fs spectroscopy , 2004 .

[2]  H. Abramczyk.  Femtosecond primary events in bacteriorhodopsin and its retinal modified analogs: revision of commonly accepted interpretation of electronic spectra of transient intermediates in the bacteriorhodopsin photocycle. , 2004, The Journal of chemical physics.

[3]  R. Mathies,et al.  Flowing liquid sample jet for resonance Raman and ultrafast optical spectroscopy , 2003 .

[4]  D. Wolpert,et al.  Protein flexibility and conformational state: a comparison of collective vibrational modes of wild-type and D96N bacteriorhodopsin. , 2003, Biophysical journal.

[5]  Marcus Motzkus,et al.  Quantum control of energy flow in light harvesting , 2002, Nature.

[6]  W. Zinth,et al.  Noncollinear optical parametric amplifiers with output parameters improved by the application of a white light continuum generated in CaF2 , 2001 .

[7]  D. Ionascu,et al.  Wavelength selective modulation in femtosecond pump-probe spectroscopy and its application to heme proteins , 2001 .

[8]  Christoph Abels,et al.  Photo-isomerisation, triplet formation, and photo-degradation dynamics of indocyanine green solutions , 1999 .

[9]  M Landthaler,et al.  Photostability and thermal stability of indocyanine green. , 1998, Journal of photochemistry and photobiology. B, Biology.

[10]  Y. H. Meyer,et al.  Transient absorption of symmetrical carbocyanines , 1998 .

[11]  Vladislav V. Yakovlev,et al.  Feedback quantum control of molecular electronic population transfer , 1997 .

[12]  R. Hochstrasser,et al.  The dynamics of photodissociation reactions in solution , 1996 .

[13]  T. Elsaesser,et al.  Vibronic excitations of large molecules in solution studied by two-color pump-probe experiments on the 20 fs time scale , 1996 .

[14]  T. Elsaesser,et al.  Vibrational and vibronic dynamics of large molecules in solution studied on a 20 fs timescale , 1995 .

[15]  V. Sundström,et al.  Femtosecond spectral evolution monitoring the bond-twisting event in barrierless isomerization in solution , 1994 .

[16]  M. Lim,et al.  Nonexponential protein relaxation: dynamics of conformational change in myoglobin. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[17]  H. Itoh,et al.  Denaturation of bacteriorhodopsin by organic solvents. , 1988, Biophysical chemistry.

[18]  J. Fouassier,et al.  Etude physicochimique d’une série de cyanines. Partie IV : rigidification, photoisomerisation et effet laser , 1982 .

[19]  K. Rothschild,et al.  [76] Kinetic resonance raman spectroscopy of purple membrane using rotating sample , 1982 .