Chiral separations on multichannel microfluidic chips

Chiral separations of FITC‐labeled basic drugs on multichannel microfluidic chips with LIF detector were investigated. A preliminary screening procedure for seven neutral CDs was performed under optimized conditions for chiral separations of three FITC‐labeled drugs (baclofen, norfenefrine, and tocainide) on a mono‐channel microfluidic chip. According to the results of screening, FITC‐baclofen and FITC‐norfenefrine as well as two chiral selectors including γ‐CD and dimethyl‐β‐CD (DM‐β‐CD) were selected as models to perform chiral separations on a two‐channel chip. FITC‐baclofen enantiomers were separated completely by γ‐CD in one channel, while resolution of FITC‐norfenefrine enantiomers was achieved by DM‐β‐CD in the other channel in the same run. Furthermore, the feasibility of using one chiral selector to separate multiple chiral samples was studied on a four‐channel chip. These results show that multichannel chip has a potential for chiral high‐throughput screening.

[1]  Sam F. Y. Li,et al.  High‐speed chiral separations on microchip electrophoresis devices , 2000, Electrophoresis.

[2]  L. Kremser,et al.  Enantiomeric resolution of galanthamine and related drugs used in anti-Alzheimer therapy by means of capillary zone electrophoresis employing derivatized cyclodextrin selectors. , 1999, Journal of chromatography. B, Biomedical sciences and applications.

[3]  Koji Otsuka,et al.  Rapid Enantioseparation of 1-Aminoindan by Microchip Electrophoresis with Linear-Imaging UV Detection , 2005, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[4]  Cheng-Huang Lin,et al.  Optimization of a simple method for the chiral separation of methamphetamine and related compounds in clandestine tablets and urine samples by beta-cyclodextrine modified capillary electrophoresis: a complementary method to GC-MS. , 2003, Forensic science international.

[5]  P. Wieczorek,et al.  Separation of aromatic aminophosphonic acid enantiomers by capillary electrophoresis with the application of cyclodextrins. , 2002, Journal of chromatography. A.

[6]  A. Manz,et al.  Micro total analysis systems. Recent developments. , 2004, Analytical chemistry.

[7]  Xiaofeng Zhu,et al.  Separation of drugs by capillary electrophoresis, Part 10. Permethyl‐alpha‐cyclodextrin as chiral solvating agent , 1999, Electrophoresis.

[8]  Frank Kohler,et al.  High‐speed chiral separations on a microchip with UV detection , 2003, Electrophoresis.

[9]  Bingcheng Lin,et al.  Simultaneous and ultrarapid determination of reactive oxygen species and reduced glutathione in apoptotic leukemia cells by microchip electrophoresis , 2005, Electrophoresis.

[10]  D. Massart,et al.  Rapid development of the enantiomeric separation of beta-blockers by capillary electrophoresis using an experimental design approach. , 1999, Journal of chromatography. A.

[11]  T. Lóránd,et al.  Chiral separation of bioactive cyclic Mannich ketones by HPLC and CE using cellulose derivatives and cyclodextrins as chiral selectors. , 2002, Journal of biochemical and biophysical methods.

[12]  S. Quake,et al.  Microfluidics in structural biology: smaller, faster ... better , 2003 .

[13]  A. Woolley,et al.  High-speed DNA genotyping using microfabricated capillary array electrophoresis chips. , 1997, Analytical chemistry.

[14]  G. Sekar,et al.  Catalyst-controlled stereoselective combinatorial synthesis. , 2003, Angewandte Chemie.

[15]  J. Goossens,et al.  Chiral capillary electrophoretic determination of the enantiomeric purity of tetrahydronaphthalenic derivatives, melatoninergic ligands, using highly sulfated β‐cyclodextrins , 2002, Electrophoresis.

[16]  Igor L. Medintz,et al.  Microfabricated 384-lane capillary array electrophoresis bioanalyzer for ultrahigh-throughput genetic analysis. , 2002, Analytical chemistry.

[17]  T. Kanamori,et al.  The use of a highly sulfated cyclodextrin for the simultaneous chiral separation of amphetamine‐type stimulants by capillary electrophoresis , 2002, Electrophoresis.

[18]  G. Blaschke,et al.  Enantioseparation of chiral vasodilator drug isoxsuprine in high-performance liquid chromatography and capillary electrophoresis. , 2002, Journal of pharmaceutical and biomedical analysis.

[19]  D. Sybilska,et al.  Comparative study on the enantiomer separation of 1,1'-binaphthyl-2,2'diyl hydrogenphosphate and 1,1'-bi-2-naphthol by liquid chromatography and capillary electrophoresis using single and combined chiral selector systems. , 2002, Journal of chromatography. A.

[20]  D. Belder,et al.  Subsecond chiral separations on a microchip , 2004, Electrophoresis.

[21]  G. Oehme,et al.  Enantiomeric resolution of derivatives of α-aminophosphonic and α-aminophosphinic acids by high-performance liquid chromatography and capillary electrophoresis , 1999 .

[22]  Suto,et al.  Designing chiral libraries for drug discovery. , 2000, Drug discovery today.

[23]  R. Galensa,et al.  Chiral separation of diastereomeric flavanone‐7‐O‐glycosides in citrus by capillary electrophoresis , 2003, Electrophoresis.

[24]  Sang-Hyo Kim,et al.  Microfluidic device for bio analytical systems , 2004 .

[25]  S. Kodama,et al.  Direct chiral resolution of aliphatic α-hydroxy acids using 2-hydroxypropyl-β-cyclodextrin in capillary electrophoresis , 1999 .

[26]  R. Mathies,et al.  Radial capillary array electrophoresis microplate and scanner for high-performance nucleic acid analysis. , 1999, Analytical chemistry.

[27]  J. Wyvratt,et al.  Comparison of capillary electrophoresis and reversed-phase liquid chromatography for determination of the enantiomeric purity of an M3 antagonist. , 2002, Journal of chromatography. A.

[28]  A. Woolley,et al.  Ultra-high-speed DNA fragment separations using microfabricated capillary array electrophoresis chips. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[29]  M. Reetz.  Combinatorial and Evolution-Based Methods in the Creation of Enantioselective Catalysts. , 2001, Angewandte Chemie.

[30]  M. Schwarz,et al.  Rapid chiral on-chip separation with simplified amperometric detection. , 2001, Journal of chromatography. A.