论文信息 - Bioaffinity separation of trypsin using trypsin inhibitor immobilized in reverse micelles composed of a nonionic surfactant.

Bioaffinity separation of trypsin using trypsin inhibitor immobilized in reverse micelles composed of a nonionic surfactant.

Trypsin inhibitor was converted to hydrophobic states by covalently combining cholesteryl groups using an acylation reaction, and was immobilized in reverse micelles composed of a nonionic surfactant. Using this reverse micellar phase containing trypsin inhibitor as an affinity ligand, trypsin was selectively separated with high recoveries from a mixture of several kinds of contaminating proteins by forward and backward extraction. No loss of activity of the recovered trypsin was observed through these operations. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 53: 406-408, 1997.

[1] J. Dordick,et al. Purification of Glycoproteins by Selective Transport Using Concanavalin‐Mediated Reverse Micellar Extraction , 1991, Biotechnology progress.

[2] William W. Cohen,et al. The preparation and properties of two new chromogenic substrates of trypsin. , 1961, Archives of biochemistry and biophysics.

[3] T. A. Hatton,et al. Affinity–based reversed micellar protein extraction: I. Principles and protein–ligand systems , 1993, Biotechnology and bioengineering.

[4] T. A. Hatton,et al. Affinity‐based reversed micellar protein extraction: II. Effect of cosurfactant tail length , 1993, Biotechnology and bioengineering.

[5] J. Dordick,et al. Affinity‐Based Reverse Micellar Extraction and Separation (ARMES): A Facile Technique for the Purification of Peroxidase from Soybean Hulls , 1993, Biotechnology progress.

[6] R. Coughlin,et al. N‐Laurylbiotinamide as Affinity Surfactant , 1990, Biotechnology progress (Print).

[7] Jyh-Ping Chen,et al. Extraction of concanavalin A with affinity reversed micellar systems. , 1994 .

[8] T. A. Hatton,et al. Bioaffinity Separations Using Reversed Micellar Extraction , 1989 .