Chapter 1 two level control of the sequence fed batch—continuous hybridoma bioreactor

In the present study a recirculation system for monoclonal antibodies production, operated consecutively fed batch and continuously, was modelled and subjected to optimal control. The optimization procedure uses a two level approach: one regarding the overall process, and two inner ones, concerning the fed batch and continuous steps. The best switch time between the two operating modes was calculated, together with the best control variable profile for each section.