Localization of Carbonic Anhydrase XII to the Basolateral Membrane of H+-secreting Cells of Mouse and Rat Kidney

Membrane-associated carbonic anhydrase (CA) has a crucial role in renal HCO3 − absorption. CA activity has been localized to both luminal and basolateral membranes of the tubule epithelial cells. CA XII is a transmembrane isoenzyme that has been demonstrated in the basolateral plasma membrane of human renal, intestinal, and reproductive epithelia. The present study was designed to demonstrate the distribution of CA XII expression in the rodent kidney. A new polyclonal antibody to recombinant mouse CA XII was used in both Western blotting and immunohistochemistry. Western blotting analysis revealed a 40–45-kD polypeptide in CA XII-expressing CHO cells and isolated membranes of mouse and rat kidney. Immunofluorescence staining localized CA XII in the basolateral plasma membranes of S1 and S2 proximal tubule segments. Abundant basolateral staining of CA XII was seen in a subpopulation of cells in both cortical and medullary collecting ducts. Double immunofluorescence staining identified these cells as H+-secreting type A intercalated cells. The localization of CA XII in the peritubular space of proximal tubules suggests that it may play a role in renal HCO3 − absorption, whereas the function of CA XII in the type A intercalated cells needs further investigation.

[1]  P. Wistrand,et al.  Carbonic anhydrase activity of isolated brush border and basal-lateral membranes of renal tubular cells , 1977, Pflügers Archiv.

[2]  M. O’Reilly,et al.  Carbonic anhydrase XII mRNA encodes a hydratase that is differentially expressed along the rabbit nephron. , 2003, American journal of physiology. Renal physiology.

[3]  W. Sly,et al.  Carbonic anhydrase XIV: luminal expression suggests key role in renal acidification. , 2002, Kidney international.

[4]  D. Ellison,et al.  Localization of thiazide-sensitive Na(+)-Cl(-) cotransport and associated gene products in mouse DCT. , 2001, American journal of physiology. Renal physiology.

[5]  V. Valderrábano,et al.  Distribution of transcellular calcium and sodium transport pathways along mouse distal nephron. , 2001, American journal of physiology. Renal physiology.

[6]  M. Soleimani,et al.  Na+:HCO3− Cotransporters (NBC): Cloning and Characterization , 2001, The Journal of Membrane Biology.

[7]  W. Sly,et al.  Expression of the transmembrane carbonic anhydrases, CA IX and CA XII, in the human male excurrent ducts. , 2001, Molecular human reproduction.

[8]  W. Sly,et al.  Expression of membrane-associated carbonic anhydrase XIV on neurons and axons in mouse and human brain. , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[9]  A. Fujimura,et al.  Role of basolateral carbonic anhydrase in proximal tubular fluid and bicarbonate absorption. , 2001, American journal of physiology. Renal physiology.

[10]  B. Fraysse,et al.  Expression of the Na(+)/Ca(2+) exchanger in skeletal muscle. , 2001, American journal of physiology. Cell physiology.

[11]  W. Sly,et al.  Expression of the Membrane-associated Carbonic Anhydrase Isozyme XII in the Human Kidney and Renal Tumors , 2000, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[12]  A. Weinstein.  A mathematical model of the outer medullary collecting duct of the rat. , 2000, American journal of physiology. Renal physiology.

[13]  G. Schwartz,et al.  Carbonic anhydrase IV is expressed in H(+)-secreting cells of rabbit kidney. , 2000, American journal of physiology. Renal physiology.

[14]  W. Sly,et al.  Expression of a novel transmembrane carbonic anhydrase isozyme XII in normal human gut and colorectal tumors. , 2000, The American journal of pathology.

[15]  W. Sly,et al.  Identification of carbonic anhydrase XII as the membrane isozyme expressed in the normal human endometrial epithelium. , 2000, Molecular human reproduction.

[16]  N. Tamura,et al.  Isolation and Characterization of CA XIV, a Novel Membrane-bound Carbonic Anhydrase from Mouse Kidney* , 1999, The Journal of Biological Chemistry.

[17]  M. Wei,et al.  Down-regulation of transmembrane carbonic anhydrases in renal cell carcinoma cell lines by wild-type von Hippel-Lindau transgenes. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[18]  G N Shah,et al.  Human carbonic anhydrase XII: cDNA cloning, expression, and chromosomal localization of a carbonic anhydrase gene that is overexpressed in some renal cell cancers. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[19]  W. Sly,et al.  Membrane‐bound carbonic anhydrase IV is expressed in the luminal plasma membrane of the human gallbladder epithelium , 1996, Hepatology.

[20]  G. Seki,et al.  On the mechanism of bicarbonate exit from renal proximal tubular cells. , 1996, Kidney international.

[21]  W. Sly,et al.  Depletion of intercalated cells from collecting ducts of carbonic anhydrase II-deficient (CAR2 null) mice. , 1995, The American journal of physiology.

[22]  W. Sly,et al.  Carbonic anhydrase IV: role of removal of C-terminal domain in glycosylphosphatidylinositol anchoring and realization of enzyme activity. , 1995, Archives of biochemistry and biophysics.

[23]  J. Libman,et al.  [Renal acidification mechanism disorders in patients with osteoporosis]. , 1995, Medicina.

[24]  H. W. Harris,et al.  Cellular and subcellular immunolocalization of vasopressin-regulated water channel in rat kidney. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[25]  V. Schuster.  Function and regulation of collecting duct intercalated cells. , 1993, Annual review of physiology.

[26]  Richard,et al.  Membrane-associated carbonic anhydrase activity in the kidney of CA II-deficient mice. , 1992, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[27]  T. Maren,et al.  Localization and activity of renal carbonic anhydrase (CA) in CA-II deficient mice. , 1991, Biochimica et biophysica acta.

[28]  W. Sly,et al.  Localization of membrane-associated carbonic anhydrase type IV in kidney epithelial cells. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[29]  R. Alpern,et al.  Apical and basolateral membrane H+ extrusion mechanisms in inner stripe of rabbit outer medullary collecting duct. , 1990, The American journal of physiology.

[30]  W. Sly,et al.  Carbonic anhydrase IV from human lung. Purification, characterization, and comparison with membrane carbonic anhydrase from human kidney. , 1990, The Journal of biological chemistry.

[31]  P. Aronson,et al.  Ionic mechanism of Na+-HCO3- cotransport in rabbit renal basolateral membrane vesicles. , 1989, The Journal of biological chemistry.

[32]  H. Jacobson,et al.  Regulation of rabbit medullary collecting duct cell pH by basolateral Na+/H+ and Cl-/base exchange. , 1989, The Journal of clinical investigation.

[33]  H. Lodish,et al.  Subtypes of intercalated cells in rat kidney collecting duct defined by antibodies against erythroid band 3 and renal vacuolar H+-ATPase. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[34]  M. Jennings,et al.  Monoclonal antibodies against human erythrocyte band 3 protein. Localization of proteolytic cleavage sites and stilbenedisulfonate-binding lysine residues. , 1986, The Journal of biological chemistry.

[35]  K. Schlüter,et al.  Colocalization of band 3 with ankyrin and spectrin at the basal membrane of intercalated cells in the rat kidney. , 1985, Science.

[36]  G. Lönnerholm,et al.  Carbonic anhydrase in the human kidney: a histochemical and immunocytochemical study. , 1984, Kidney international.

[37]  P. A. Friedman,et al.  Carbonic anhydrase histochemistry in rabbit and mouse kidneys , 1982, The Anatomical record.

[38]  Y. Ridderstråle,et al.  Intracellular distribution of carbonic anhydrase in the rat kidney. , 1980, Kidney international.

[39]  R. Vernier,et al.  Tamm-Horsfall glycoprotein: ultrastructural immunoperoxidase localization in rat kidney. , 1979, Laboratory investigation; a journal of technical methods and pathology.

[40]  D. McKinley,et al.  Particulate carbonic anhydrase in homogenates of human kidney. , 1976, Biochimica et biophysica acta.

[41]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.